Effect of follicle stimulating hormone and testosterone on viability rate of cryopreserved spermatogonial stem cell after thawing

Iranian Journal of Veterinary Science and Technology

مقاله 3، دوره 5، شماره 1، بهمن 2013، صفحه 26-34   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v5i1.12101

نویسندگان

Reza Narenji Sani^{* 1}؛ Parviz Tajik¹؛ Mohammad Hasan yousefi²؛ Mansoureh Movahedin³؛ Shiva Shafiei¹

¹University of Tehran

²Semnan University

³Tarbiat Modarres University

چکیده

Stem cells are generally defined as clonogenic cells capable of both self-renewal and differentiation. Probably the best method for long-term preservation of spermatogonial stem cells is cryopreservation. In this study, effects of Follicle Stimulating Hormone and Testosterone on viability rate of cryopreserved spermatogonial stem cell after Thawing were investigated. Sertoli and spermatogonial cells were isolated from 3-5 months old calves. Cocultured sertoli and spermatogonial cells were treated with Follicle Stimulating Hormone and Testosterone in treatment groups before cryopreservation. Results indicated that Follicle Stimulating Hormone increased viability rate of cryopreserved spermatogonial cells in
comparison with Testosterone and control group. In conclusion, using Follicle Stimulating Hormone provided proper bovine spermatogonial stem cell culture medium for in vitro
culture and cryopreservation of these cells.

کلیدواژه‌ها

Cryopreservation؛ Spermatogonial Stem Cell؛ Bovine؛ Follicle Stimulating Hormone؛ Testosterone

مراجع

Anway, M.D., Folmer, J., Wright, W.W.and Zirkin, B.R.(2003) Isolation of sertoli Cells from Adult Rat Testes: An Approach to Ex Vivo Studies of Sertoli Cell Function. Biology of Reproduction 68, 996-1002.

Baarends, W.M., Grootegoed, J.A. (1999)Molecular biology of male gametogenesis. Parthenon, NewYork.pp 271-295.

Bellve, A.R., Cavicchia, J.C., Millette, C.F.,32 O'Brien, D.A., Bhatnagar, Y.M.andDym, M.(1977)Spermatogenic cells of the prepuberal mouse: isolation and morphological characterization. The Journal of Cell Biology 74, 68-85.

deRooij, D.G.(1973)Spermatogonial Stem Cell Renewalinthe Mouse: I. Normal Situation. Cell Proliferation 6, 281-287.

Dirami, G., Ravindranath, N., Pursel, V.and Dym, M.(1999) Effects of Stem Cell Factor and Granulocyte Macrophage-Colony Stimulating Factor on Survival of Porcine Type A Spermatogonia Cultured in KSOM.Biology of Reproduction 61, 225-230.

Huang, H.F.S. andNieschlag, E.(1986)Suppression of the Intratesticular Testosterone Is Associated with Quantitative Changes in Spermatogonial Populations in Intact Adult Rats. Endocrinology 118, 619-627.

Huckins, C.(1971) The Spermatogonial Stem CellPopulationin Ault Rats. Cell Proliferation 4, 313-334.

Izadyar, F., Matthijs-Rijsenbilt, J.J., Ouden, K.D., Creemers, L.B., Woelders, H.and de Rooij, D.G.(2002a) Development of a Cryopreservation Protocol for Type A Spermatogonia. Journal of Andrology 23, 537-545.

Izadyar, F., Spierenberg, G.T., Creemers, L.B., den Ouden, K. and de Rooij,D.G.(2002b) Isolation and purification of type A spermatogonia from the bovine testis. Reproduction 124, 85-94.

Jousan, F.D., Oliveira, L.J., Hansen and P.J.(2008) Short-Term culture of in vitro produced bovine preimplantation embryos with insulin-like growth factor-i prevents heat shock-induced apoptosis through activation of the Phosphatidylinositol 3-Kinase/Akt

pathway. Molecular Reproduction and Development 75, 681-688.

Koruji, S.M., Movahedin, M., Mowla, S.J. and Gourabi, H. (2007) Colony formation ability of frozen thawed spermatogonial stem cell from adult mouse. Iranian Journal of Research Medicine 5, 109-115.

Kubota, H., Avarbock, M.R. andBrinster, R.L.(2004) Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells. Proceedings of the National Academy of Sciences of the United States of America 101,16489-16494.

Lejeune, H., Chuzel, F., Thomas, T., Avallet,O., Habert, R., Durand, P. and Saez, J.(1996) Paracrine regulation of Leydig cells. Endocrinology 57, 55-63.

McLean, D.J., Russell, L.D. and Griswold,M.D.(2002) Biological Activity and Enrichment of Spermatogonial Stem Cells in Vitamin A-Deficient and Hyperthermia-Exposed Testes from Mice Based on Colonization Following Germ Cell Transplantation. Biology of Reproduction 66, 1374-1379.

Michael D, G.(1998) The central role of Sertoli cells in spermatogenesis. Seminars in Cell & Developmental Biology 9, 411-416.

Morena, A.R., Boitani, C., Pesce, M., De Felici, M. andStefanini, M.(1996) Isolation of highly purified type A spermatogonia from prepubertal rat testis. Journal of Andrology 17, 708-717.

NarenjiSani, R., Tajik, P., yousefi, M.H.,Movahedin, M., Qasemi-Panahi, B. and Shafiei, Sh. (2012a) Follicle-Stimulating Hormone increase spermatogonial stem cell colonization during In vitro co-culture. Accepted in Veterinary Research Forum.

NarenjiSani, R., Tajik, P., yousefi, M.H. and Movahedin, M. (2012b) Effect of testosterone on spermatogonial cell

colony formation during In vitro coculture.Accepted in Iranian Journal of Veterinary Medicine.

Oakberg, E.F.(1971) Spermatogonial stem-cell renewal in the mouse.The Anatomical Record 169, 515-531.

Skinner, M.K. (2005) Sertoli cell secreted regulatory factors. In Sertoli Cell Biology. Elsevier Science, San Diego.pp 107-120.

Tajik, P., Barin, A., Movahedin, M., Zarnani, A., Hadavi, R., Moghaddam, G., Shoja, J., Jeddi-Tehrani, M., Ashrafi-Helan, J., Heidari-Vala, H., Torkabadi, E. and Qasemi-Panahi, B.(2010)Nestin, a neuroectodermal stem cell marker, is expressed by bovine sertoli cells.Comparative Clinical Pathology, 1-5.

van Pelt, A.M., Morena, A.R., van Dissel-Emiliani, F.M., Boitani, C., Gaemers,I.C., de Rooij, D.G. and Stefanini,M.(1996) Isolation of the synchronized A spermatogonia from adult vitamin deficient rat testes. Biology of Reproduction 55, 439-444.

Walker, W.H. and Cheng, J.(2005) FSH and testosterone signaling in Sertoli cells.Reproduction 130, 15-28.

Wrobel, K. -H., Bickel, D., Kujat, R. and Schimmel, M.(1995) Evolution and ultrastructure of the bovine spermatogonia precursor cell line. Cell and Tissue Research 281, 249-259.