Histopathological study of avian tuberculosis in naturally infected domestic pigeons with Mycobacterium avium subsp. avium

Iranian Journal of Veterinary Science and Technology

مقاله 4، دوره 5، شماره 1، بهمن 2013، صفحه 45-56   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v5i1.13796

نویسندگان

Mansour Mayahi¹؛ Saleh Esmaeilzadeh²؛ Nader Mosavari³؛ Kaveh parvandar asadollahi^{* 1}

¹Shahid Chamran University of Ahvaz

²University of Ahvaz

³Razi Vaccine and Serum Research Institute

چکیده

The aim of this study was to investigate the histopathology of avian tuberculosis in naturally infected domestic pigeons (Columba livia var. domestica) with Mycobacterium aviumsubsp.avium. Avian tuberculosis is one of the most important diseases that affect all species of birds, and is most often caused by mycobacterium avium and mycobacterium genavense. Eighty out of more than 600 pigeons were selected based on their clinical signs and poor healthconditions and under standard conditions were euthanized, necropsied, and followed by bacterial culture on specific media for Mycobacterium avium subsp.avium. Fifty Mycobacterium avium subsp. Avium was isolated from pigeons. All acid fast bacilli isolates were tested by the PCR assays targeting the 16S rRNA, IS1245 and IS901 genes. After definitive identification of Mycobacterium aviumsubsp. avium by culturing and PCR assay, 45 fixed samples including liver, gizzard, proventriculus, intestines, kidneys and lungs from positive pigeons were subjected for histopathology studies. Tissues sections were prepared as usual and stained by hematoxylin and eosin, Ziehl-Neelsen and Congo red. Based on gross findings, liver and intestines were the most affected organs. Histologically, caseative uncalcified granulomatous inflammation was noted in the affected organs. Also histopathology examinations showed that most of the granulomatous lesions in the lungs were in microscopic size and it seems that lungs were affected more than it was expected. In Ziehl-Neelsen’s staining, a large number of acid-fast bacilli were observed within multinucleated giant cells and in necrotic areas. Also in Congo red staining, deposition of amyloid in liver and kidneys sections were observed. In conclusion, histopathology findings were typical of avian tuberculosis, including acid fast bacilli and uncalcified caseous necrosis centers which were surrounded by multinucleated giant cells, macrophages and lymphocytes.

کلیدواژه‌ها

Pigeon؛ Mycobacterium aviumsubsp.avium؛ Amyloid؛ granulomatous lesions؛ Acid-fast bacilli

مراجع

Byrd, T.F. (1998)Multinucleated giant cell formation induced by IFN-γ/IL-3 is associated with restriction of virulent Mycobacterium tuberculosiscell-to-cell invasion in human monocytes monolayers. Cellular Immunology 188,89-96.

Cotran, R.S., Kumar, V., and Collins, T.(1999) Robbins Pathologic Basis of Diseases. Philadelphia, PA: WB Saunders Co.251-259.

Cowper Coles, P., Cicuta, M. E., Zumarraga,M., Etchetchoury, I., Lertora, J.,Ramirez, G.V. (2007) Avian mycobacteriosis in chicken from a rural area of Argentina. RevistaVeterinaria 18, 72-77.

Dannenberg, A.M. (2006) Pathogenesis of Human Tuberculosis. Insights from the Rabbit Model. Washington, DC: American Society for Microbiology Press.

Dvorska, L., Bull, T. J., Bartos, M., Matlova, L., Svastova, P.,Weston, R. T., Kintr, J., Parmova, I., Van Soolingen, D.,

Pavlik, I. (2003) A standardized restriction fragment length polymorphism (RFLP) method for typing Mycobacterium avium isolates links IS901 with virulence for birds. Journal of Microbiological Methods 55, 11–27.

Dvorska, L., Matlova, L., Ayele, W.Y., Fischer, O.A., Amemori, T., Weston, R.T., Alvarez, J., Beran, V.,Moravkova, M., Pavlik, I. (2007)Avian tuberculosis in naturally infected captive water birds of the Ardeideae and Threskiornithidae families studied 54 Mayahi M., et al.by serotyping, IS901 RFLP typing, and virulence for poultry. Veterinary Microbiology 119, 366-374.

Fulton, R., Sanchez, S. (2008)Tuberculosis, pp. 940-941. In Y.M Saif, H.J Barnes, J.R Glisson, A.M Fadly, L.R McDougald, D.E Swayne. (Eds). Disease of poultry. 12th edition. Blackwel publishing.

Guerrero, C., Bernasconi, C., Burki, D., Bodmer, T., Telenti, A.(1995) A novel insertion element from Mycobacterium avium, IS1245, is a specific target for analysis of strain relatedness. Journal of Clinical Microbiology 33, 303-304.

Ikonopoulos, J., Fragkiadaki, E., Liandris, E., Sotirakoglou, K.,Xylouri, E., Gazouli, M. (2009) Estimation of the spread of pathogenic mycobacteria in organic broiler farms by the polymerase chain reaction. Veterinary Microbiology.278-282.

Kantor, I.N.D., Kim, S.J., Frieden, T., Laszlo, A., Luelmo, F., Yvesnorval, P., Rieder, H., Valenzuela, P., Weyer, K. (1998) Laboratory services in tuberculosis control, Microscopy part ΙΙ.World Heaith Organization.

Kunze, Z.M., Wall, S., Appelberg, R., Silva, M.T., Portaels, F., McFadden, J.J. (1991)IS901, a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity in Mycobacterium avium.Molecular Microbiology 5, 226-227.

Luna, Lee G. (1968) Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rded. McGraw- Hill Book Company. pp. 34-36, 153-155, 220.

Meyerholz, D.K., Vanloubbeeck, Y.E., Hoistetter, S.J., Jordan, D.M.,Fales- Williams, A.J.(2005) Surveillance of amyloidosis and other diseases at necropsy in captive trumpeter swans (Cygnus buccinator). Journal of Veterinary Diagnostics and Investigation 17,295-298.

Montali, R.J., Bush, M., Thoen, C.O., Smith,E. (1976): Tuberculosis in captive exotic birds. Journal of the American Veterinarian Medical Association 169,920-927.

North, R.J. & Jung Y.J. (2004) Immunity to tuberculosis. Annual Review in Immunology 22, 599-623.

Salfinger, M., and Kafader, F.M. (1987):Comparison of two pretreatment methods for the detection of Mycobacteria of BACTEC and Lowenstein-Jensen slants. Journal of Microbiological Methods 6, 315–321.

Shitaye. J. E., Halouzka, R., Svobodova, J.,Grymova, V., Grym, M., Skoric, M., Fictum, P., Beran, V., Slany, M.,Pavlik, I. (2010) First isolation of Mycobacterium genavense in a blue headed parrot (Pionusmenstruus)imported from Surinam (South America) to the Czech Republic: a case report 55, 339-347.

Skoric, M., Fictum, P., Frgelecova, L., Kriz, P., Slana, I., Kaevska, M and Pavlik, I. (2010) Avian tuberculosis in a captured Ruppell’s griffon vulture (Gypsruppellii).VeterinarniMedicina 55,348–352.

Smith, M.B., Boyars, M.C., Veasey, S.,Woods, G.L. (2000)Generalized tuberculosis in the acquired immune deficiency syndrome. A clinicopathologic analysis based on autopsy findings. Archives of Pathology and Laboratory Medicine 124, 1267-1274.

Tell, L.A., Foley, J., Needham, M.L., Walker, R.L. (2003) Diagnosis of avian mycobacteriosis; comparison of culture, acid-fast stains, and polymerase chain reaction for the identification of Mycobacterium avium in experimentally inoculated Japanese quail (Coturnixcoturnix japonica). Avian Diseases 47, 444-452.

Tell, L.A., Woods, L., Cromie, and R.L.(2001) Scientifiqueet Technique Office International des Epizooties 20, 180-203.

Thorel, M.F., Huchzermeyer, H., Weiss, R.,Fontaine, J.J. (1997) Mycobacterium avium infections in animals. Veterinary Research 28, 439-447.

Van Dar Heyden, N. (1997) Clinical manifestations of ycobacteriosis in pet birds. Seminars in Avian and Exotic

Pet Medicines 6, 18-24.

Van Soolingen, D., Hoogenboezem, T., De Haas, P.E.W., Hermans, P.W.M., Koedam, A., Teppema, K.S., Brennan,

P.J., Besra, G.S., Portaels, F., Top, J., Schouls, L.M. & Van Embden, J.D.A. (1997) A novel pathogenic taxon of the

Mycobacterium tuberculosis complex, canettii: characterization of an exceptional isolate from Africa.International Journal of Systematic and Evolutionary Bacteriology 47, 1236-1245.