Effects of Gonadotropin Releasing Hormone (GnRH) on bovine spermatogonial stem cell proliferationatogonial stem cells proliferation

Iranian Journal of Veterinary Science and Technology

مقاله 2، دوره 6، شماره 2، فروردین 2014، صفحه 11-20   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v6i2.21007

نویسندگان

Parviz Tajik¹؛ Hesam Kohsari^{* 1}؛ Babak Qasemi-Panahi²؛ Iraj Sohrabi-Haghdoost³؛ Abbas Barin⁴؛ Hamid Ghasemzadeh-Nava⁴

¹Islamic Azad University

²University of Tabriz

³Science and Research‌ Branch, Islamic Azad University

⁴University of Tehran

چکیده

Identification of exogenous factors affecting spermatogonial stem cells (SSCs) proliferation in vitro, provides worthy ways to study the basic biology of the cells. The aim of this study was to investigate the effects of a GnRH analogue (alareline acetate) on SSCs colonization in short-term co-culture with sertoli cells. Five, three-month old Holstein male calves were used to isolate spermatogonial and sertoli cells. Testicular germ cell collection was made by enzymatic digestion methods. The cells were co-cultured in a 15 day period and in vitro effects of various doses (0.5, 1, 2 and 4 µg/ml) of GnRHa on SSCs colonization were assessed. Effects of GnRHa on SSCs proliferation were dose dependent. In conclusion, it was demonstrated that 1 µg/ml GnRHa was the optimum dose for SSCs colonization in comparison with control group. The highest treatment dose (4 µg/ml GnRHa), negatively affected SSCs colonization in comparison with control group.

کلیدواژه‌ها

GnRH؛ Calf؛ Spermatogonial Stem Cell؛ Sertoli cell؛ Co-culture

مراجع

Anjamrooz, S.H., Movahedin, M., Tiraihi, T. and Mowla, S.J. (2006). In vitro effects of epidermal growth factor, follicle stimulating hormone and testosterone on mouse spermatogonial cell colony formation. Journal of Reproduction, Fertility and Developmen 18, 709-720.

 

Anway, M.D., Folmer, J., Wright, W.W. and Zirk, B.R. (2003). Isolation of sertoli cells from adult rat testes: an approach to ex vivo studies of sertoli cells Function. Journal of Biology of reproduction 68, 996-1002.

 

Billing, H., Furuta, I. and Hsueh, A.J. (1994). Gonadotropin-releasing hormone directly induces apoptotic cell death in the rat ovary: biochemical in situ detection of deoxyribonucleic acid fragmentation in granulose cells. Journal of Endocrinology 134, 245-252.

 

Bourne, G.A., Regiani, S., Payne, A.H. and Marshall, J.C. (1980). Testicular GnRH receptors: characterization and localization on interstitial tissue. Journal of Clinical Endocrinology and Metabolism 51, 407-409.

 

Bull, P., Morales, P., Huyser, C., Socias, T. and Castellon, E.A. (2000). Expression of GnRH receptor in mouse and rat testicular germ cells. Journal of Molecular Human Reproduction 6, 582-586.

 

de Rooij, D.G. (2006). Rapid expansion of the spermatogonial stem cell toolbox. Journal of Cell biology 103, 21, 7939-7940.

 

Dobrinski, I., Ogawa, T., Avarbock, M.R and Brinster, R.L. (2001). Effect of the GnRH-agonist, leuprolide on colonization of recipient testes by donor spermatogonial stem cells after transplantation in mice. Journal of Tissue and Cell 33 (2), 200-207.

 

Dym, M. and Cavicchia, J.C. (1997). Further observations on the blood- testis barrier in monkeys. Journal of Biology of reproduction 17, 390-403.

 

Griswold, M.D. (1995). Interactions between germ cells and sertoli cells in the testis. Journal of Biology of Reproduction 52, 211-216.

 

Hermann, B.P., Sukhwani, M., Lin, C., Sheng, Y., Tomko, J., Rodriguez, M., Shuttleworth, J.J., Mc Farland, D., Hobbs, R.M; Pandolfi, P.P., Schatten, G.P and Orwig, K.E. (2007). Characterization, cryopreservation, and ablation of spermatogonial stem cells in adult rhesus Macaques. Journal of Stem Cells 25, 2330-2338.

 

Hsien-Ming, W., Hsin-Shih, W., Hong-Yuan, H., Yung-Kuei, S., Mac Calman, C. and Leung, P.C.K. (2009). GnRH signaling in intrauterine tissues. Journal of Reproduction 137, 769-777.

 

Izadyar, F., Spierenberg, G.T., Creemers, L.B., Den Ouden, K. and de Rooij, D.G. (2002). Isolation and purification of typeA spermatogonia from the bovine testis. Journal of Reproduction 124, 85-94.

 

Izadyar, F., Den Ouden, K., Creemers, L.B., Posthuma, G., Parvinen, M. and de Rooij, D.G. (2003). Proliferation and differentiation of bovine type A Spermatogonia during long-term culture. Journal of Biology of reproduction 68, 272-281.

 

Khaira, H., Mc Lean, D., Ohl, D.A. and Smith, G.D. (2005). Spermatogonial stem cell isolation, storage and transplantation. Journal of Andrology 26 (4), 442-450.

 

Koko, H., Kudo, A., Park, M.K., Mori, T. and Kawashima, S. (1995). In situ detection of gonadotropin releasing hormone (GnRH) receptor mRNA expression in the rat ovarian follicles. Journal of Experimental Zoology 272, 62-68.

 

Koruji, M., Movahedin, M., Movla, S.J., Gourabi., H. and Arfaee, A.J. (2009). Efficiency of adult mouse spermatogonial stem cell colony formation under several culture conditions. Journal of In vitro Cellular and Developmental Biology- Animal 45, 281-289.

 

Kubota, H., Avarbock, M.R. and Brinster, R.L. (2004). Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells. Journal of Proceedings of the National Academy of Sciences of the United States of America 101, 16489-16494.

 

Kubota, H. and Brinster, R.L. (2006). Technology insight: in vitro culture of spermatogonial stem cells and their potential therapeutic uses. Journal of Nature Clinical Pratice 2, 2, 99-108.

 

Mather, J.P., Attie, K.M., Woodruff, T.K., Rice, G.C. and Phillips, D.M. (1990). Activin stimulates spermatogonial proliferation in germ -sertoli cell co-cultures from immature rat testis. Jounal of Endocrinology 127, 3206-3214.

 

Meachem, S., von Schonfeldt, V. and Schlatt, S. (2001). Spermatogonia: stem cells with a great perspective. Journal of Reproduction 12, 825-834.

 

Millar, R.P. (2005). GnRHs and GnRH receptors. Journal of Animal Reproduction Science 88, 5-28.

 

Narenji Sani, R., Tajik, P., Yousefi, M.H., Movahedin, M., Qasemi-Panahi, B., Shafiei, S. and Ahmadi Hamedani, M. (2012a). Follicle stimulating hormone increases spermatogonial stem cell colonization in vitro co-culture. Journal of Veterinary Research Forum 4(1), 37-41.

 

Narenji Sani, R., Tajik, P., Yousefi, M.H. and Movahedin, M. (2012b). Effect of testosterone on spermatogonial cell colony formation during in vitro co-culture. Iranian Journal of Veterinary Medicine 6(3), 159-164.

 

Ogawa, T., Dobrinski, I., Avarbock, M.R. and Brinster, R.L. (1998). Leuprolide, a gonadotropine-releasing hormone agonist, enhances colonization after spermatogonial transplantation into mouse testis. Journal of Tissue and Cell 30, 583-588.

 

Ogawa, T., Dobrinski, I. and Brinster, R.L. (1999). Recipient preparation is critical for spermatogonial transplantation in the rat. Journal of Tissue and Cell 31, 461-472.

 

Pellegrini, M., Grimaldi, P., Rossi, P., Geremia, R. and Dolci, S. (2003). Developmental expression of BMP4/ALK3/ SMAD5 signaling pathway in the mouse testis: a potential role of BMP4 in spermatogonia differentiation. Journal of Cell Science 116, 3363-3372.

 

Ramakrishnappa, N., Rajamahendran, R., Lin, Y. and Leung, P.C.K. (2005). GnRH in non-hypothalamic reproductive tissues. Journal of Animal Reproduction Science 88, 95-113.

 

Ryu, B., Kubota, H., Avarbock, M.R. and Brinster, R.L. (2005). Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat. Journal of Cell Biology 102, 40, 14302-14307.

 

Saragueta, P.E., Lanuza, G.M. and Raranao, J.L. (1997). Inhibitory effect of gonadotropin-releasing hormone (GnRH) on rat granulose cells deoxyribonucleic acid synthesis. Journal of Molecular Reproduction and Development 47, 170-174.

 

Shafiei, Sh., Tajik, P., Ghasemzadeh Nava, H., Movahedin, M., Talebkhan Garoussi, M., Qasemi Panahi, B. and Rahimi Feyli, P. (2013). Isolation of bovine spermatogonial cells and co-culture with prepubertal sertoli cells in the presence of colony stimulating factor-1. Iranian Journal of Veterinary Medicine 7(2), 83-90.

 

Tajik, P., Barin, A., Movahedin, M., Zarnani, A.H., Hadavi, R., Moghaddam, G., Shoja, J., Jeddi-Tehrani, M., Ashrafi-Helan, J., Heidari-Vala, H., Torkabadi, E. and Qasemi-Panahi, B. (2010). Nestin, a neuroectodermal stem cell marker, is expressed by bovine sertoli cells. Journal of Comparative Clinical Pathology 21,395-399.

 

Van Dissel-Emiliani, F.M., Grootenhuis, A.J., de Jong., F.H. and de Rooij., D.B.(1989). Inhibin reduces spermatogonial numbers in testes of adult mice and Chinese hamsters. Journal of Endocrinology 125, 1899-1903.