Karyotypic characterization of the pike, Esox lucius from the south Caspian Sea basin

Iranian Journal of Animal Biosystematics

مقاله 4، دوره 11، شماره 1، دی 2015، صفحه 43-49   

نوع مقاله: Research articles

شناسه دیجیتال (DOI): 10.22067/ijab.v11i1.46485

نویسندگان

Majidreza Khoshkholgh¹؛ Alireza Alavi¹؛ Sajad Nazari^{* 2}

¹Department of Fisheries, Faculty of Natural Resources, University of Guilan, P.O. Box 1144 Sowmehsara, Iran

²Genetic and Breeding Research Center for Coldwater Fishes, P.O. Box 75914-358 Yasouj, Iran

چکیده

The karyotype of pike from Anzali lagoon in the south Caspian Sea basin have been investigated for the first time by conventional chromosome staining. The chromosomes spreads were stained with 7% Giemsa solution for 15 min and examined under a light microscope. Appropriate metaphase plates were photographed in order to prepare karyotype. All samples had a diploid number of 50 chromosomes (2n=50), with a karyotype consist of 12 metacentric, 24 submetacentric, 14 subtelocentric and fundamental number (NF) of 86. The largest chromosome in this species was a pair of metacentric chromosomes. Based on type of chromosomes, the karyotype of this species was nearly differed with what that found in previous studies, which could be attributed to the existence of different populations for this species. Despite the conserved diploid number, the data on the cytogenetic structure help characterize the karyotype of this species.

کلیدواژه‌ها

Esox lucius؛ Caspian Sea؛ Cytogenetic؛ Giemsa staining

مراجع

Abbasi, K., Valipour, A., Talebi Haghighi, D., Sarpanah, A. and Nezami, Sh. 1999. Atlas of Iranian Fishes. Guilan Inland Waters. Guilan Fisheries Research Centre, Rasht. 113 pp. (In persian).

 

Arkhipchuk, V.V. 1999. Chromosome database. Database of Dr. Victor Arkhipchuk. www.fishbase.org.

 

Coad, B. W. 2006. Endemicity in the freshwater fishes of Iran. Iran. Journal of Animimal Biosystematics. 1: 1-13.

 

Collares-Pereira, M.J., Prospero, M.I., Bileu, M.I. and Rodrigues, E.M. 1998. Leuciscus (Pisces, Cyprinidae) karyotypes: transect of Portuguese populations. Genetics and Molecular Biology. 21: 63–69.

 

Crossman, E.J. and Rab, P. 1996. Chromosome-banding study of the Alaska blackfish, Dallia pectoralis (Euteleostei: Esocae), with implication for karyotype evolution and relationship of esocoid fishes. Canadian Journal of Zoology. 74:147–156.

 

Fontana, F. 2002. A cytogenetic approach to the study of taxonomy and evolution in sturgeons. Journal of Appllied Ichthyology. 18 (2002), 226–233.

 

Jankun, M ., Woznicki, P., Dajnowicz, G., Demska-Zakes, K., Luczynski, M. J. and Luczynski, M. 1998. Heterochromatin and NOR location in northern pike (Esox lucius). Aquatic science. 60 (1998) 17–21.

 

Jolodar, M. N. and Abdoli, A. 2004. Fish Species Atlas of South Caspian Sea Basin (Iranian Waters). Iranian Fisheries Research Organization, Teheran. 110 pp.

 

Kalbasi, MR., Dorafshan, S., Tavakolian,T., Khazab, M & Abdolhay, H. 2006. Karyological analysis of endangered Caspian salmon, Salmo trutta caspius (Kessler, 1877). Aquaculture research. 37, 1341-1347.

 

Kiabi, B. H., Abdoli, A. and Naderi, M. 1999. Status of the fish fauna in the South Caspian Basin of Iran. Zoology in Middle East. 18: 57-65.

 

King, M. 1993. Species evolution: the role of chromosome change. Cambridge University. Press, Cambridge, pp. 336.

 

Levan, A., Fredga, K. and Sandberg, A.A. 1964. Nomenclature for centromeric position on chromosomes. Hereditas. 52: 201–220.

 

Luczynski, M.J., Glogowski, J., Kucharczyk, D., Luczynski, M. and Demska-Zakes, K. 1997. Gynogenesis in northern pike (Esox lucius L.) induced by heat shock-preliminary data. Poliske Arch Hydrobiologie 44.

 

Mayr, B. 1987. Chromosome banding studies in European esocid fishes: localization of NOR in Umbra krameri and Esox lucius. Copeia 1987:1062–1067.

 

Nazari, S., Pourkazemi, M and Porto, J.I.R. 2009. Comparative cytogenetic analysis of two Iranian cyprinids Alburnoides bipunctatus and Alburnus filippii (Cypriniformes: Cyprinidae). Iran. Journal of Animimal Biosystematics 5(2), 23-32.

 

Nazari, S., Pourkazemi, M and Porto, J.I.R. 2011. Chromosome description and localization of Nucleolus Organizing Regions by Ag-staining Technique in Alburnus filippii (Cyprinidae, Cypriniformes) of the south Caspian Sea Basin, Guilan, Iran. Iranian Journal of fisheries science, 10(2), 352-356.

 

Nejatsanatee, A. R. 1994. Ameerkalaye Lagoon in Lahijan, Abzeeyan, Tehran, 5(1 & 2):2-4, II-III. (In Persian).

 

Nelson, J.S. 1994. Fishes of the World. J. Wiley & Sons Inc. New York, pp. 176–177.

 

Nygren, A. 1968. Cytological studies in perch (Perca fluviatilis L.), pike (Esox lucius), pike perch (Lucioperca lucioperca) and ruff (Acerina cernua). Hereditas 59, 518–524.

 

Pourkazemi, M., Nazari, S., Bakhshalizadeh S. 2010. Karyotype analysis in white bream Blicca bjoerkna from north coast of Iran. Iranian Journal of Fisheries Science 9(3) 463-454.

 

Rab, P. and Crossman, E. J. 1994. Chromosomal NOR phenotypes in North American pikes and pickerels genus Esox, with notes on the Umbridae (Euteleostei: Esocae). Canadian Journal of Zoology. 72:1951–1956.

 

Rab, P. and P. Roth, (1988) Cold-blooded vertebrates. In: P. Balicek, J. Forejt and J. Rubes (Editors) Methods of Chromosome Analysis. Cytogenet. Sect. Cs. Biol. Soc., Brno, pp. 115–124.

 

Riazi, B. 1996. Siah-Keshim. The Protected Area of Anzali Wetland. Department of the Environment, Tehran. 101 pp. ( In Persian).

 

Vasil’ev, V. P. and Vasil’eva, E. D. 2008. Comparative Karyology of Species of the Genera Misgurnus and Cobitis (Cobitidae) from the Amur River Basin in Connection with Their Taxonomic Relations and the Evolution of Karyotypes. Journal of Ichthyology. 48, (1). 1–13.

 

Zou Sh. and Li ,S. F. 2006. The study on chromosomes of white spot pike (Esox lucius). Journal of Shang Fisheries University. 2, 203-211.