Cytogenetic study and pollen viability of three populations of Diplotaxis harra (Brassicaceae) in Iran

Journal of Cell and Molecular Research

مقاله 4، دوره 6، شماره 2 - شماره پیاپی 12، فروردین 2014، صفحه 93-98   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v6i2.34453

نویسندگان

Massoud Ranjbar^* ؛ Somayeh Karami

Bu-Ali Sina University

چکیده

In this study, we examined the chromosome number, detailed male meiosis, microsporogenesis, pollen fertility and distribution of cytotypes of Diplotaxis harra (Forssk.) Boiss. This is the first cytogenetic report of the taxon. The two of the populations scored now from regions of the central showed aneuploid (x = 14) meiotic chromosome count and one of the populations studied from the existed at the diploid level (x = 13). It seems that the species with x = 14 are derived through aneuploid increase. The most prominent among these meiotic abnormalities was the chromatin stickiness which involved inter PMC and fragmented chromosome at different stages of meiosis. Consequently, these populations exhibited varying percentages of pollen sterility and pollen grains of smaller sizes. The aim of the present research was to study the male meiosis in detail and to find the impact of chromatin stickiness in inducing meiotic aberrations and their consequent effect on pollen fertility and pollen size, also to differentiate the individuals growing wild and also to find out the distribution patterns of different cytotypes in the Iran.

کلیدواژه‌ها

Brassicaceae؛ chromosome number؛ cytotypes؛ Diplotaxis؛ Iran؛ meiotic

مراجع

Al Achkar W., Sabatier L. and Dutrillaux B. (1989) How are sticky chromosome formed? Annales de genetique 30: 10-15.

Al-ShehbazI. (1978) Chromosome number reports in certain Cruciferae from Iraq. Journal of Biological Sciences 6: 26-31.

Al-Shehbaz I. (2012) A generic and tribal synopsis of the Brassicaceae (Cruciferae). Taxon 61: 931-954.

Al-Shehbaz I. and Al-Omar M. (1982) In IOPB chromosome number reports LXXVI. Taxon 31: 587-589.

Appel O. and Al-Shehbaz I. (2003) Cruciferae. In: Kubitzki K. and Bayer C. eds., In: The families and genera of vascular plants. Springer, Berlin.

Badr A. and Ibrahim A. (1987) Effect of herbicide glean on mitosis, chromosomes and nucleic acids in Allium cepa and Viciafaba root meristems. Cytologia 52: 293-302.

Baptists-Giacomelli F., Pagliarini M. and Almeida J. (2000) Elimination of micronuclei from microspores in a Brazilian oat (Avena sativa L.) variety. Genetic Molecular of Biology 23: 681-684.

Caetano-Perira C., Pagliarini M. and Brasil E. (1995) Influence of aluminium in causing chromosome stickiness in maize microsporocytes. Maydica 40: 324-330.

Falistocco E., Tosti N. and Falcinelli M. (1995) Cytomixisin pollen mother cells of diploid Dactylis, one of the origins of 2n gametes. Cytologia 86: 448-453.

Gomez-Campo C. (1999) Taxonomy. In: Gomez-Campo C. ed., Biology of Brassica coenospecies. Elsevier, Amsterdam.

Hedge I. (1968) Brassicaceae. In: Rechinger K. H. ed., Flora Iranica, vol. 57.AkademischeDruck-u. Verlagsanstalt, Graz.

Hedge I., Lamond J. and Townsend C. (1980) Cruciferae. In: Flora of Iraq eds. Townsend C. and Guest E., Flora Iraq. 4(2). Ministry of Agriculture and Agrarian Reform, Baghdad.

Koduru P. and Rao M. (1981) Cytogenetics of synaptic mutant in higher plants. Theoretical and Applied Genetics 59: 197-214.

Lattoo S., Khan S., Bamotra S. and Dhar A. (2006) Cytomixis impairs meiosis and influences reproductive success in Chlorophytumcomosum (Thunb) Jacq., an additional strategy and possible implications. Journal of Bioscience 31: 629-637.

Malallah G. and Attia T. (2003) Cytomixis and its possible evolutionary role in a Kuwaiti population ofDiplotaxisharra (Brassicaceae). Botanical Journal of the Linnean Society 143(2): 169-175.

Mok D. and Peloquin S. (1975) The inheritance of three mechanism of diplandroid (2n pollen) formation in diploid potatoes. Heredity 35: 295-302.

Mok D., Peloquin S. and Mendiburu A. (1976) Genetic evidence for mode of 2n pollen formation and S-locus mapping in potatoes. Potato Research 19: 157-164.

Nirmala A. and Rao P. (1996) Genetics of chromosome numerical mosaism in higher plants. The Nucleus 39: 151-175.

Peres Kiihl P. (2011) Chromosome stickiness during meiotic behavior analysis of Passifloraserrato-digitata L. (Passifloraceae). Ciência Rural 41: 1018-1023.

Polatschek A. (1983) Chromosomenzahlen und hinweise auf systematik und verbreitung van Brassicaceae-Artenaus Europa, Nordafrika, Asien und Australien. Phyton (Horn) 23: 127–139.

Ramana M. (1974) The origin of unreduced microspores due to aberrant cytokinesis in the meiocytes of potato and its genetic significance. Euphytica 23: 20-30.

Rao P. and Rao R. (1977) Gamma-ray induced meiotic chromosome stickiness in tomato. Theoretical and Applied Genetics 50: 247-252.

Shabrangi A., Sheidai M., Majd A., Nabiuni M. and Dorranian D. (2010) Cytogenetic abnormalities caused by extremely low frequency electromagnetic fields in canola. Science Asia 36: 292-296.

Snogerup B. (1985) Chromosome Number Reports LXXXIX. Taxon 34: 727-730.

Steffensen D. (1955) Breakage of chromosomes in Tradescantia with calcium deficiency. Proceeding of Natural Academy Science of USA 41: 155-160.

Steffensen D. (1956) Effects of various cation imbalances on the frequency of x-ray induced chromosomal aberrations in Tradescantia. Genetics 42: 239-252.

Warwick S. and Al-Shehbaz I. (2006) Brassicaceae: chromosome number index and database on CD-Rom. Plant Systematics and Evolution 259: 237-248.

Warwick S. and Sauder C. (2005) Phylogeny of tribe Brassiceae (Brassicaceae) based on chloroplast restriction site polymorphisms and nuclear ribosomal internal transcribed spacer and chloroplast trnL intron sequences. Canadian Journal Botany 83: 467-483.

Wilson G. (1945) The venetian turpentine mounting medium. Stain Technology 20: 133-135.

Zanella C. (1991) Differential effect of soil acidity and lime treatment on the chromosomes of two wheat cultivars. Brazil journal Genetics 14: 1021-1032.