Acipenser Persicus Growth Hormone gene Sequencing and its structures

Journal of Cell and Molecular Research

مقاله 2، دوره 7، شماره 1 - شماره پیاپی 13، مهر 2015، صفحه 47-52   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v7i1.41102

نویسندگان

Ehsan Nasr^{* 1}؛ Mohammad Pourkazemi²؛ Hrachia Hovhannisyan³

¹Islamic Azad University of Meshkin Shahr

²Iranian Fisheries Research Organization, Iran

³Scientific and Production Center “Armbiotechnology NAS RA

چکیده

Administering growth hormone increases growth rate of cultured fish. The aim of this study was isolation and synthesize of Persian sturgeon GH cDNA. The total RNA was extracted from pituitary gland of Persian sturgeon and cDNA was synthesized. The full-length cDNA sequence of Persian sturgeon contains a 645 nucleotide open reading frame, which encoding a 214 amino acid residues. The position of the signal peptide cleavage site was predicted to be at position 72. After cleaving of a signal peptide of 24 amino acid resi dues, a mature peptide of 190 aa formed. The blast observed that the PS pre GH have highest nucleotide sequence similarity with Acipenseridae family and mammals GH. Secondary structure and tertiary structure of Persian sturgeon growth hormone gene were prediction by online software. The secondary structure of the GH was revealed the predomination of a- helix (> 55%), the domains of high conservation across the vertebrate GH protein. The predicted 3D structure of Persian sturgeon growth hormone attribute to the typical 4-a-helix bundle protein conformation, the characteristic 3-D confirmation of growth hormones.

کلیدواژه‌ها

Persian sturgeon؛ growth hormone؛ cDNA