بررسی فوزاریوم های بذرزاد پیاز در استان های خراسان شمالی و رضوی

پژوهش های حفاظت گیاهان ایران

مقاله 3، دوره 24، شماره 2 - شماره پیاپی 8، تیر 1389   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v24i2.4123

نویسندگان

الهه ربیعی مطلق^* ؛ ماهرخ فلاحتی رستگار¹؛ حمید روحانی²؛ بهروز جعفرپور؛ وحید جهانبخش²

¹گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد

²دانشگاه فردوسی مشهد

چکیده

Abstract
Fusarium species are one of the most important pathogens on onion in Iran and out of whit few are transmitted by onion seeds. In order to identify onion seed borne Fusaria in Razavi and Northern Khorasan provinces, in the September 2007 seed samples from different field of four localities were studied using cultural media, greenhouse assay and molecular technique respectively. In the first experiment surface disinfested and nonsterlized onion seeds were cultured on PDA, in the second experiment surface disinfested seeds were planted in outoclaved soil under greenhouse condition.. F. proliferatum and F. oxysporum were detected and isolated from seeds cultured on PDA and seedlings grown in greenhouse. The rate of F. proliferatum transmitted by seed found to be 0-15 and 0- 6.7 % on PDA and greenhouse respectively, while it was between 0-48 and 0-10 %. for F. oxysporum. For molecular studies after extracting F. proliferatum DNA isolated from seed samples and performing PCR by selecting specific primers, molecular weights of bands on agarose gel were compared using the labwork 3,0,2 software. Distinctive difference were observed between molecular weights of bands from diverse regions. A positive significant correlation was also observed between the results of culture media and molecular methods. The result of this study confirmed that F. proliferatum and F. oxysporum are both seed borne on onion.

Key word: Onion, Seed borne, Fusarium, Khorasan, Molecular detection