Systems Biology Analysis of the Key Genes of Surfactin Production in Bacillus subtilis MJ01 (Isolated from Soil Contaminated Oil in South of Iran), Spizizenii, and 168 Isolates

Journal of Cell and Molecular Research

مقاله 4، دوره 9، شماره 2 - شماره پیاپی 18، تیر 2017، صفحه 84-97   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v9i2.66166

نویسندگان

Tahereh Deihimi^{* 1}؛ Esmaeil Ebrahimie²؛ Ali Niazi¹؛ Mansour Ebrahimi³؛ Shahab Ayatollahi⁴؛ Ahmad Tahmasebi⁵؛ Touraj Rahimi⁵؛ Moein Jahanbani Veshareh⁶

¹Shiraz university

²Shiraz uiversity and University of Adelaide

³University of Qom

⁴Sharif University of Technology

⁵Shiraz University

⁶Denmark technichal university

چکیده

Applying microorganism in oil recovery has attracted attentions recently. Surfactin produced by Bacillus subtilis is widely used industrially in a range of industrial applications in pharmecutical and environmental sectors. Little information about molecular mechanism of suffactin compound is available. In this study, we performed promoter and network analysis of surfactin production genes in Bacillus subtilis subsp. MJ01 (isolated from oil contaminated soil in South of Iran), spizizenii and 168. Our analysis revealed that comQ and comX are the genes with sequence alterations among these three strains of Bacillus subtilis and are involved in surfactin production. Promoter analysis indicated that lrp, argR, rpoD, purr and ihf are overrepresented and have the highest number of transcription factor binding sites (TFBs) on the key surfactin production genes in all 3 strains. Also the pattern of TFBs among these three strains was completely different. Interestingly, there is distinct difference between 168, spizizenii and MJ01 in their frequency of TFs that activate genes involve in surfactin production. Attribute weighting algorithms and decision tree analysis revealed ihf, rpoD and flHCD as the most important TF among surfactin production. Network analysis identified two significant network modules. The first one consists of key genes involved in surfactin production and the second module includes key TFs, involved in regulation of surfactin production. Our findings enhance understanding the molecular mechanism of surfactin production through systems biology analysis.

کلیدواژه‌ها

Surfactin production gene؛ Transcription factor (TF)؛ Promoter analysis؛ Network analysis

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