Induction of AHR Gene Expression in Colorectal Cancer Cell Lines by Cucurbitacin D, E, and I

Journal of Cell and Molecular Research

مقاله 3، دوره 10، شماره 2 - شماره پیاپی 20، خرداد 2019، صفحه 67-75   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v10i2.74806

نویسندگان

Younes Aftabi¹؛ Habib Zarredar²؛ Mohammadreza Sheikhi³؛ Zahra Khoshkam³؛ Abasalt Hosseinzadeh Colagar^{* 4}

¹Tabriz University of Medical Sciences; University of Mazandaran, Babolsar, Mazandaran

²Tabriz University of Medical Sciences, Tabriz, Iran

³University of Tehran, Tehran, Iran

⁴University of Mazandaran, Babolsar, Mazandaran, Iran

چکیده

     Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor and its induction may result in suppressing of cell proliferation in colorectal cancer (CRC). Cucurbitacin D (CucD), E (CucE) and I (CucI) are plant derived metabolites that inhibit cancer cells. This study aimed to evaluate the possible potency of the cucurbitacins for activation of AHR expression in CRC cell lines SW-480 and HT-29. The MTT assay was used to find the LC50 value of the metabolites in the cell lines. Afterward, the cells incubated with the LC50 concentrations and AHR-mRNA expression assessed using RT-PCR. The LC50 values of CucD, CucE, and CucI were 4.5, 6.8, and 3.8 μM in HT-29 cell line and 35, 19, 17.5 μM in SW-480 cells respectively. The SW-480 cells were more resistant against Cucs in comparison with HT-29 cells and all three Cucs cause to more AHR-mRNA expression in HT-29 cells. CucE had the lowest effect on AHR-mRNA expression in the cell lines and CucI was a common metabolite for both HT-29 and SW-480 cells, which showed the lowest LC50 value (the highest toxicity) and the highest effect on AHR-mRNA expression. CucI may have a potential to be used as an activator of AHR expression in CRC studies.

کلیدواژه‌ها

Aryl hydrocarbon receptor؛ Colon cancer؛ Cucurbitacin؛ HT-29؛ SW-480

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