High prevalence of Prototheca spp. and isolation of fungal species in milk samples from cows suffering from mastitis in Mashhad city, northeast Iran

Iranian Journal of Veterinary Science and Technology

مقاله 3، دوره 11، شماره 2 - شماره پیاپی 21، شهریور 2019، صفحه 21-26   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v11i2.81838

نویسندگان

Maryam Lavaee¹؛ Samaneh Eidi^{* 2}؛ Babak Khoramian³

¹Graduated from the Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

²Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

³Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده

The aim of this study was to investigate the fungi and algae isolated from milk samples in dairy cows with clinical and subclinical mastitis from dairy farms around Mashhad, Iran. A total of 503 milk samples were obtained from 10 industrial dairy farms. All samples were simultaneously cultured on the surface of Blood agar, Macconkey agar, and Sabouraud dextrose agar supplemented with chloramphenicol. Fungi and algae were identified using phenotypic characteristics. In the examined samples, the bacterial contamination (338 out of 503 samples; 67.20%) was the most dominant followed by algae (93 out of 503 samples; 18.5%), filamentous fungi (32 out of 503 samples; 6.4%) and yeast fungi (26 out of 503 samples; 5.2%).Penicillium spp. (2.8%), Aspergillus spp. (2.6%), Cladosporium spp. (1.2%), Geotrichum spp.(0.4%), Ulucaladium spp., Scopolariopsis spp. and Alternaria spp. (0.2% each), Cryptococcus neofermenes (3%), Candida spp. (1%), Trichosporon spp. (0.8%) and Rhodoturula spp. (0.4%) were the filamentous and yeast fungi isolated from milk samples. Of the 93 algae isolated from 503 milk samples (18.5%), 83 (16.5%) samples were positive for Prototheca zopfii and 10 (2%) samples for Prototheca wickerhamii. According to the results of this study, yeast fungi, filamentous fungi and algae, especially Prototheca spp. are important contaminant factors in milk and contribute to clinical and subclinical bovine mastitis. Therefore, proper sanitation practices and management of dairy herds and judicious use of antibiotics is essential to control the fungal and algal contamination both in the environment and the breast.

کلیدواژه‌ها

mastitis؛ cattle؛ Fungi؛ algae؛ Prototheca spp

مراجع

1. Eldesouky I, Mohamed N, Khalaf D, Salama A, Elsify A, Ombarak R, et al. Candida mastitis in dairy cattle with molecular detection of Candida albicans. Kafkas Univ Vet Fak Derg. 2016; 22(3):461-464.

2. Krukowski H. Mycotic mastitis in cows. 2001; 57(1):18–20.

3. Costa EO, Ribeiro AR, Watanabe ET, Melville PA. Infectious bovine mastitis caused by environmental organisms. J Vet Med B. 1998; 45(2):65–71.

4. Jones GM. Understanding the Basics of Mastitis. http://pubs.ext.vt.edu/404/404-233/404-233.html.

5. Spanamberg A, Wunder EA Jr, Brayer Pereira DI, Argenta J, Cavallini Sanches EM, Valente P, et al. Diversity of yeast from bovine mastitis in Southern Brazil. Rev Iberoam Micol. 2008; 25 (3): 154-156.

6. Bakr EM, El-Tawab AE, Elshemey TM, Abd-Elrhman AH. Diagnostic and therapeutic studies on mycotic mastitis in cattle. Alex J Vet. 2015; 46: 138-145.

7. Cosmina C, Rapuntean Gh, Rapuntean S, Fit N, Nadas G, Vilela C. Florescent in situ hybridization (FISH) method optimization for rapid detection of Prototheca in clinical samples. Bull UASVM Vet Med 2008; 65:248–252.

8. Ahrholdt J, Murugaiyan J, Straubinger RK, Jagielski T, Roesler U. Epidemiologi- analysis of worldwide bovine, canine and human clinical Prototheca isolates by PCR genotyping and MALDI-TOF mass spectrometry proteomic phenotyping. Med Mycol. 2012; 50: 234–43.

9. Talebkhan Garoussi M, Khosravi AR, Hovarashti P. The survey of mycotic flora of uterine cows with reproductive disorders and healthy. J Vet Res. 2008; 63 (1): 7-10.

10. Radostits OM, Gay CC, Blood DC, Hinchcliff KW. Veterinary Medicine: A Text book of Disease of cattle, sheep, pigs, Goats and Horses 9th ed. W B Saunders London 2000; 603-660.

11. Diversity of yeasts and coliforms associated with bovine subclinical mastitis in periurban dairy farms in Kaduna Metropolis, Kaduna state, Nigeria [Ph.D. thesis]. Ahmadu Bello University, Zaria, Nigeria; 2017.

12. Elad D, ShpigelNY, Winkler M, Klinger I, Fuchs V, Saran A, et al. Feed contamination with Candida krusei as a probably source of mycotic mastitis in dairy cows. J Am Vet Med Assoc 1995; 207, 620-622.

13. Divers TJ, Peek SF. Rebhun’s Diseases of Dairy Cattle. 3rd ed. Elsevier Saunders; 2018.

14. Kalinska A, Golębiewski M, Wojcik A. Mastitis pathogens in dairy cattle – a review. World Scientific News. 2017; 89: 22-31.

15. Casia dos Santos R, Marine JM. Isolation of candida spp. from mastitis bovine milk in Brazil. Mycopathologia. 2005; 159: 251-253.

16. Blowey R, Edmondson P. Mastitis Control in Dairy Herds. 2nd Ed, CAB International, Cambridge; 2010.

17. Krzyżanowski J, Sielicka B. The characteristics of anascogenic yeasts isolated from the clinical cases of mastitis in cows. Ann Univ Mariae Curie-Sklodowska (Vet) 1996; 51, 59-63.

18. Krukowski H, Lisowski A, Rozanski P, Skorka A. Yeasts and algae isolated from cows with mastitis in the south-eastern part of Poland. Pol J Vet Sci. 2006; 9 (3):181-184.

19. Zaragoza CS, Olivares RA, Watty AE, Moctezuma Ade L, Tanaca, LV. Yeasts isolation from bovine mammary glands under different mastitis status in the Mexican High Plateu. Rev IberoamMicol.2011; 28 (2):79-82.

20. Suhyla, T, Seyhan, K. The slime production by yeasts isolated from subclinical mastitic cows. Acta Vet Brno. 2010; 79: 581-586.

21. Talebkhan Garoussi M, Khosravi, AR, Pandamoz S. Milk mycoflora survey of dairy cows with or without mastitis. Iran J Vet Sci Technol.2013; 4(1), 69-74.

22. Rasouli A. survey on prevalence rate of fungal species in cattle mastitis at some dairy farm around Tabriz city. Int J Biol Pharm Allied Sci. 2016; 5(4): 900-906.

23. Batavani RA, Ovnagh AG and Nikkhah, MA. An Investigation on fungal mastitis in Dairy Cattle in Urmia. The Second Iranian Cattle and Buffalo Seminar 2002.

24. Milanov DU, Prunic BO, Velhner MA, Diagnosis of yeast mastitis in dairy cows. Lucrari Stiintifice Medicina Veterinara, 2014, XLVLL (1).

25. Pachauri S, Varshney P, Dash SK and Gupta MK. Involvement of fungal species in bovine mastitis in and around Mathura, India. Vet World. 2013; 6(7): 393-395.

26. Singh S, Sood N, Gupta PP, Jand SK, Banga HS. Experimental candidial mastitis in goats: clinical, haematological, biochemical and sequential pathological studies. Med Mycol. 1998; 140: 89-97.

27. Milanov D, Petrovic T, Polacek V, Suvajdzic L, Bojkovski J. Mastitis associated with Prototheca zopfii - an emerging health and economic problem on dairy farms. J Vet Res. 2016; 60: 373-378.

28. Jagielski T, Roeske K, Bakuła Z, Piech T, Wlazlo L, Bochniarz M, et al. A survey on the incidence of Prototheca mastitis in dairy herds in Lublin province, Poland. J dairy sci. 2019; 102 (1): 619-628.

29 Moller Truyen U, Roesler U, Veterinary microbiology. 2007; 120(3): 370-374.

30. Zaini A, Kanani M, Falahati R, Fateh M, Salimi-Asl N, Saemi Sh, et al. Identification of Prototheca zopfii from Bovine Mastitis. Iran J Public Health. 2012; 41(8): 84-88.

31. Wawron W, Bochniarz M, Piech T, Wysocki J, Kocik M, Bulletin-Veterinary Institute In Pulawy. 2013; 57: 485-488.