Hematology of the Levant Green Frog, Pelophylax bedriagae (Amphibia: Ranidae) in southern Iran

Iranian Journal of Animal Biosystematics

مقاله 4، دوره 16، شماره 2 - شماره پیاپی 29، مهر 2020، صفحه 155-170   

نوع مقاله: Research articles

شناسه دیجیتال (DOI): 10.22067/ijab.v16i2.85390

نویسندگان

Behzad Fathinia^{* 1}؛ Tahereh Minaei¹؛ Hossein Javanbakht²؛ Arya Shafaeipour¹

¹Department of Biology, Faculty of Science, Yasouj University, Yasouj, Iran

²Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran

چکیده

In this study, 68 specimens of the Levant Green Frog, Pelophylax bedriagae, were collected from agricultural fields around Yasouj and Noor-Abad cities, from August 2018 to May 2019 in southern Iran to investigate the blood cell morphology. Blood specimens were taken from the heart, or abdominal and facial veins. Smears were dried in room temperature, fixed by methanol and stained using Giemsa method. Blood cells (erythrocytes and leucocytes) were counted and measured using light microscopy under an optical micrometer. Based on the findings of this study, erythrocytes from Noor-Abad population has significantly greater values from those in Yasouj population in all of the six metric characters (P < 0.05). RBC’s in male frogs are also greater than those in females in all six studied characters (P < 0.05). All studied metric characters in lymphocytes are also different between tow localities and sexes (P < 0.05).

کلیدواژه‌ها

RBC؛ Leucocyte؛ Yasouj؛ Noor-Abad؛ Hematology

مراجع

Allender, M.C., Fry, M.M., 2008. Amphibian hematology. Veterinary Clinics of North America: Exotic Animal Practice 11(3), 463‒480.

Altland, P.D., Brace, K.C., 1962. Red cell life span in the turtle and toad. American Journal of Physiology-Legacy Content 203(6), 1188‒1190.

AmphibiaWeb. 2019. https://amphibiaweb.org>, University of California, Berkeley, CA, USA, 6 Sep 2019.

Arıkan, H., Çiçek, K., 2010. Morphology of peripheral blood cells from various species of Turkish Herpetofauna. Acta Herpetologica 5(2), 179‒198.

Arikan, H., Çiçek, K., 2014. Haematology of amphibians and reptiles: A review. North-Western Journal of Zoology 10(1), 190‒209.

Arıkan, H., 1989. Anadolu’daki Rana ridibunda (Anura: Ranidae) populasyonlarının kan hücrelerinin sayısı bakımından incelenmesi. Turkish Journal of Zoology 13, 54‒59.

Arikan, H., Atatür, M.K., Tosunoğlu, M., 2003. A study on the blood cells of the Caucasus Frog, Pelodytes caucasicus. Zoology in the Middle East 30(1), 43‒47.

Arıkan, H., Çevik, İ.E., Kaya, U., Mermer, A., 2001. Anadolu Dağ Kurbağalarında Eritrosit Ölçümleri.

Arserim, S.K., Mermer, A., 2008. Hematology of the Uludağ Frog, Rana macrocnemis Boulenger, 1885 in Uludağ National Park (Bursa, Turkey). Su Ürünleri Dergisi 25(1), 39‒46.

Atatür, M.K., Arikan, H., Çevi̇k, I.E., 1999. Erythrocyte sizes of some anurans from Turkey. Turkish Journal of Zoology 23(2), 111‒114.

Bamezar, F., Fathinha, B., Shafaeipour, A., 2019. Trophology of Levant Green Frog, Pelophylax bedriagae (Amphibia: Anura: Ranidae) in Choram Township, Kohgilouyeh & Boyer-Ahmad Province, Iran. North-western Journal of Zoology 15(2), 168‒174.

Baraquet, M., Grenat, P.R., Salas, N.E., Martino, A.L., 2013. Intraspecific variation in erythrocyte sizes among populations of Hypsiboas cordobae (Anura, Hylidae). Acta Herpetologica 8(2), 93‒97.

Barros, M.M., Pezzato, L.E., Kleemann, G.K., Hisano, H., Rosa, G.J.d.M., 2002. Níveis de vitamina C e ferro para tilápia do Nilo (Oreochromis niloticus). Revista Brasileira de Zootecnia 31(6), 2149‒2156.

Cathers, T., Lewbart, G.A., Correa, M., Stevens, J.B., 1997. Serum chemistry and hematology values for anesthetized American bullfrogs (Rana catesbeiana). Journal of Zoo and Wildlife Medicine 171‒174.

Christopher, M.M., Berry, K.H., Wallis, I., Nagy, K., Henen, B., Peterson, C., 1999. Reference intervals and physiologic alterations in hematologic and biochemical values of free-ranging desert tortoises in the Mojave Desert. Journal of wildlife diseases 35(2), 212‒238.

Claver, J.A., Quaglia, A.I., 2009. Comparative morphology, development, and function of blood cells in nonmammalian vertebrates. Journal of exotic pet medicine 18(2), 87‒97.

Das, M., Mahapatra, P.K., 2014. Hematology of wild-caught Dubois’s tree frog Polypedates teraiensis, Dubois, 1986 (Anura:Rhacophoridae). The Scientific World Journal https://doi.org/10.1155/2014/491415.

Davis, A., Maney, D., Maerz, J., 2008. The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology 22(5), 760‒772.

Dönmez, F., Tosunoğlu, M., Gül, Ç., 2009. Hematological values in hermaphrodite, Bufo bufo (Linnaeus, 1758). North-Western Journal of Zoology 5(1), 97‒103.

Erişmiş, U., Arikan, H., Alpagut-Keskin, N., Çevik, İ.E., 2010. A study on the blood cells of the fire-bellied toad, Bombina bombina L.(Anura: Bombinatoridae). Animal biology 60(1), 61‒68.

Evans, G., 1939. Factors influencing the oxygen consumption of several species of plethodontid salamanders in aerial and aquatic media. Ecology 74‒95.

Hartman, F., Lessler, M., 1964. Erythrocyte measurements in fishes amphibia, and reptiles. The Biological Bulletin 126(1), 83‒88.

Hutchison, V.H., Haines, H.B., Engbretson, G., 1976. Aquatic life at high altitude: respiratory adaptations in the Lake Titicaca frog, Telmatobius culeus. Respiration physiology 27(1), 115‒129.

Iwama, G., Nakanishi, T., 1996. The immune system. California: Academic Press. 380p.

Javanbakht, H., Vaissi, S., Parto, P., 2013. The morphological characterization of the blood cells in the three species of turtle and tortoise in Iran. Research in Zoology 3(1), 38‒44.

Kiesecker, J.M., 2002. Synergism between trematode infection and pesticide exposure: a link to amphibian limb deformities in nature? Proceedings of the National Academy of Sciences 99(15), 9900‒9904.

LeaMaster, B.R., Brock, J.A., Fujioka, R.S., Nakamura, R.M., 1990. Hematologic and blood chemistry values for Sarotherodon melanotheron and a red hybrid tilapia in freshwater and seawater. Comparative Biochemistry and Physiology Part A: Physiology 97(4), 525‒529.

Manning M.J., Horton J.D., 1982. RES Structure and Function of the Amphibia. In: Cohen N., Sigel M.M. (eds) Phylogeny and Ontogeny. Springer, Boston, MA.

Martins, M., Mouriño, J., Amaral, G., Vieira, F., Dotta, G., Jatobá, A., Pedrotti, F., Jerônimo, G., Buglione-Neto, C., 2008. Alterações hematológicas em tilápia do Nilo infectada experimentalmente com Enterococcus sp. Brazilian Journal of Biology 68(3), 657‒661.

Martins, M., Tavares-Dias, M., Fujimoto, R., Onaka, E., Nomura, D., 2004. Haematological alterations of Leporinus macrocephalus (Osteichtyes: Anostomidae) naturally infected by Goezia leporini (Nematoda: Anisakidae) in fish pond. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 56(5), 640‒646.

Monnickendam, M.A., Balls, M., 1973. The relationship between cell sizes, respiration rates and survival of amphibian tissues in long-term organ cultures. Comparative Biochemistry and Physiology Part A: Physiology 44(3), 871‒880.

Peng, F., Zhang, R., Zhu, X., Wang, H., Zhang, S., 2016. Hematology and serum biochemistry of farmed bullfrog, Lithobates catesbeianus during the active and hibernating periods. Journal of Veterinary Medicine and Animal Health 8(11), 176‒182.

Pesarakloo, A., Rastegar-Poyani, E., Rastegar-Poyani, N., Kami, H., Najibzadeh, M., Khosravani, A., Oraie, H., 2017. The first taxonomic revaluation of the Iranian water frogs of the genus Pelophylax (Anura: Ranidae) using sequences of the mitochondrial genome. Mitochondrial DNA 28(3), 392‒398.

Ruiz, G., Rosenmann, M., Veloso, A., 1983. Respiratory and hematological adaptations to high altitude in Telmatobius frogs from the Chilean Andes. Comparative Biochemistry and Physiology Part A: Physiology 76(1), 109‒113.

Ruiz, G., Rosenmann, M., Veloso, A., 1989. Altitudinal distribution and blood values in the toad, Bufo spinulosus Wiegmann. Comparative biochemistry and physiology A, Comparative physiology 94(4), 643‒646.

Saint Girons, M.C., 1970. Morphology of the circulating blood cells. Biology of the Reptilia 3(2), 73‒92.

Shutler, D., Marcogliese, D.J., 2011. Leukocyte profiles of northern leopard frogs, Lithobates pipiens, exposed to pesticides and hematozoa in agricultural wetlands. Copeia 2011(2), 301‒307.

Smith, H.M., 1925. Cell size and metabolic activity in Amphibia. The Biological Bulletin 48(5), 347‒378.

 Storer, T.I., Usinger, R.L., Stebbins, R.C., Nybakken, J.W., 1957. General zoology, McGraw-Hill.

 Szarski, H., Czopek, G., 1966. Erythrocyte diameter in some amphibians and reptiles. Bulletin De L Academie Polonaise Des Sciences-Serie Des Sciences Biologiques 14(6), 433.

 Tavares-Dias, M., De Moraes, F.R., Onaka, E.M., Rezende, P.C.B., 2007. Changes in blood parameters of hybrid tambacu fish parasitized by Dolops carvalhoi (Crustacea, Branchiura), a fish louse.  Veterinarski arhiv 77(4), 355‒363.

 Thrall, M.A., Weiser, G., Allison, R., Campbell, T., 2012. Veterinary hematology and clinical chemistry. John Wiley & Sons.

 Tierney, K., Farrell, A., Kennedy, C., 2004. The differential leucocyte landscape of four teleosts: juvenile Oncorhynchus kisutch, Clupea pallasi, Culaea inconstans and Pimephales promelas. Journal of Fish Biology 65(4), 906‒919.

 Vasaruchapong, T., Disarapong, P., Chulasugandha, P., Khow, O., Chanhome, L., Chiobamroongkiat, M., Chaiyabutr, N., Sitprija, V., 2014. Comparative studies on hematological and plasma biochemical parameters in different types of venomous snakes in Thailand. Comparative Clinical Pathology 23(4), 955‒959.

 

Vernberg, F.J., 1955. Hematological studies on salamanders in relation to their ecology. Herpetologica 11(2), 129‒133.

 Weber, R.E., 2007. High-altitude adaptations in vertebrate hemoglobins. Respiratory physiology & neurobiology 158(2‒3), 132‒142.

 Wojtaszek, J., Baranowska, M., Glubiak, M., Dzugaj, A., 1997. Circulating blood parameters of the water frog, Rana esculenta L. at pre-wintering stage. Zoologica Poloniae 42, 1‒4.

 Wright, K.N., Whitaker, B.R., 2001.  Amphibian medicine and captive husbandry. Krieger Publication Company, 1 edition, 570p.