Estradiol benzoate priming during induction of estrus with Vitex-castus extract in dogs

Iranian Journal of Veterinary Science and Technology

مقاله 2، دوره 12، شماره 2 - شماره پیاپی 23، اسفند 2020، صفحه 10-18   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v12i2.85480

نویسندگان

Ala Ghadery؛ Esmail Ayen؛ Alireza Kabirian^*

Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

چکیده

This study compared two methods of estrus induction between dogs (using vitagnus and vitagnus-estradiol). A total of 10 adult cyclic female Shih tzu Terrier mix breed dogs at anestrus stage were selected and divided into two groups. The first group (VAC) received vitagnus for five weeks (90 mg daily, PO). The second group (VAC-E2) was treated with vitagnus and estradiol benzoate. Estradiol benzoate was injected at the beginning of each week (0.01 mg/kg, IM). Blood sampling for evaluation of steroid hormones and vaginal smears were taken weekly. The sings and return to the estrus with the number of puppies were recorded. In the VAC group, 60%, and in the VAC-E2 group, 80% of dogs returned to the follicular phase after five weeks. In the VAC-E2 group, signs of estrus appeared 7 to 10 days and a mating process started 4 to 6 days earlier than those in the VAC group. The average number of delivered puppies was 4. These symptoms were confirmed by the cytology of the vagina. There was no significant difference in the estradiol and progesterone levels between groups. The mean concentration of estradiol significantly changed between weeks 1 and 4, 1 and 5, and 2 and 5 (p < 0.05). The progesterone level in the VAC-E2 group on week five was higher than that in other weeks. In conclusion, the administration of estradiol benzoate before  vitagnus improved induction of estrus in dogs.

کلیدواژه‌ها

estrus induction؛ estradiol benzoate؛ Vitex-castus extract؛ dog

مراجع

1. Mogheiseh A, Nazifi S, Ahmadi MR, et al. Comparing the Vitex-castus extract and cabergoline effects on estrous induction in female dogs. Comp Clin Path 2017;26(5):1099-103.

2. Bouchard G, Youngquist R, Clark B, et al. Estrus induction in the bitch using a combination diethylstilbestrol and FSH-P. Theriogenology 1991;36(1):51-65.

3. Gunay A, Gunay U, Soylu M. Cabergoline applications in early and late anestrus periods on German Shepherd dogs. Revue de medecine veterinaire 2004;155:557-60.

4. Jeukenne P, Verstegen J. Termination of dioestrus and induction of oestrus in dioestrous nonpregnant bitches by the prolactin antagonist cabergoline. J Reprod Fertil Suppl 1997;51:59-66.

5. Kutzler MA. Induction and synchronization of estrus in dogs. Theriogenology 2005;64(3):766-75.

6. Rodas-Ruiz J, Tabares-Serna C, Giraldo-Echeverri C. Evaluacion de un protocolo con prostaglandin F2a, benzoato de estradiol y acetato de deslorelina para la induccion de estro en perras. Archivos de medicina veterinaria 2015;47(3):395-9.

7. Park S-J, Kim T-S, Kim J-M, et al. Repeated superovulation via PMSG/Hcg administration induces 2-Cys peroxiredoxins expression and overoxidation in the reproductive tracts of female mice. Mol. Cells 2015;38(12):1071.

8. Concannon P. Induction of fertile oestrus in anestrous dogs by constant infusion of GnRH agonist. J Reprod Fertil Suppl 1989;39:149-60.

9. Concannon P. Biology of gonadotrophin secretion in adult and prepubertal female dogs. J Reprod Fertil Suppl 1993;47:3-27.

10. Barta M, Archbald L, Godke R. Luteal function of induced corpora lutea in the bitch. Theriogenology 1982;18(5):541-9.

11. Hossein-Rashidi B, Nemati M. Effects of Vitex agnus-castus extract on the secretory function of pituitary-gonadal axis and pregnancy rate in patients with premature ovarian aging (POA). J Herb Med 2017;10:24-30.

12. Zahid H, Rizwani GH, Ishaqe S. Phytopharmacological review on Vitex agnus-castus: a potential medicinal plant. Chinese Herbal Medicines 2016;8(1):24-9.

13. Diab AE-AA, Elsayed ZI, Zahra MH, et al. Biological study of the extract of some species of vitex agnus-castus (kafmurium) grown in Egypt. IJPSR; 2015.

14. Niroumand MC, Heydarpour F, Farzaei MH. Pharmacological and therapeutic effects of Vitex agnus-castus L.: A review. Pharmacogn Rev 2018;12(23):103.

15. MAYO JL. Black cohosh and chasteberry: herbs valued by women for centuries. target 1998;19:22-6.

16. Mogheiseh A, Ghiri MJM, Bandarian E. The Clinical Follow-Up of Estradiol Benzoate Priming During Induction of Estrus With Cabergoline in Dogs. Top Companion Anim Med 2017;32(1):16-9.

17. Antonov A. Application of exfoliative vaginal cytology in clinical canine reproduction-a review. Bulg. J. Vet. Med 2017;20(3).