Serological and Molecular Detection of Tomato spotted wilt virus (TSWV) in Khorasan Razavi Province

پژوهش های حفاظت گیاهان ایران

Article 1, Volume 26, Issue 4 - Serial Number 18, December 2013, Pages 348-354   

Document Type: Research Article

DOI: 10.22067/jpp.v26i4.18418

Authors

S. Shoushtari^* ; B Jafarpour; M. Falahaty Rastegar

Abstract

In order to investigate the presence of Tomato spotted wilt virus ( TSWV) in tomato fields of Khorasan Razavi province ( Mashhad , Neishabour , Torbat-e-Hydarieh , Kashmar , Ghochan , Fariman , Takht-e-Golgeh and Roshtkhar ), totally 636 samples were collected from tomato fields in summer of 2008 and 2009 . The plant that showed symptoms of chlorotic , necrotic and ring spot were collected and transferred to the laboratory in cold condition , then tested by TAS-ELISA . Sap from infected plants inoculated to 3 cultivars of indicator plant e.g Nicotiana rustica, N . clevelandii and N. tabacum var Samsun . To confirme symptomology, inoculated indicator plants tested by TAS-ELISA. For molecular detection, RNA extracted by use of RNX TM(plus) kit . In RT-PCR , specific primer , amplified a fragment of 276 bp . Using RT-PCR and mechanical transmission tests , the presence of TSWV has been proven in tomato field of the province .In inoculating indicator plants only Nicotiana tabacum var Samsun (leaf deforming and mottling ) showed specific virus symptoms .

Keywords

Mechanical transmission; RT-PCR; Tomato spotted wilt virus