Application of Microsatellite Markers for Identification and Registration of Walnut Cultivars

علوم باغبانی

Article 16, Volume 28, Issue 4, November 2014, Pages 584-593   

Document Type: Research Article

DOI: 10.22067/jhorts4.v0i0.25874

Authors

Mohsen Mardi¹; Mehrshad Zeinalabedini^{* 1}; Rohollah Haghjoyan²; Seyyed Hassan Jamali³; Seyyed Mojtaba Khayam Nekouei⁴; Abdolreza Kavand³; Karim Ahmadi⁴; Leila Sadeghi³; Ali Akbar Loni⁵; Tayebe Karami⁵; Soghra Khoshkam⁵

¹Agricultural Biotechnology Research Institute of Iran (ABRII)

²Plant and Seed Improvement Institute, Karaj

³Seed and Plant Certification and Registration Institute, Karaj

⁴Agricultural Biotechnology Research Institute of Iran

⁵Adjutancy of Plant Production of the Ministry of Jihad-e-Agriculture

Abstract

Due to the complex assessment of young walnut cultivars (Juglansregia L.) based on morphological traits, advance molecular tools have provided a new prospect for cultivar identification and DNA fingerprinting. In this study, specific molecular keys were identified for 5 Iranian walnut cultivars (Juglansregia L.) using 30 SSR markers. The results showed that 5 SSR markers produced polymorphic bands for studied Iranian walnut cultivars. SSR markers WP-376andABRII-WM-6produced specific molecular keys in walnut cultivars K72, Z30, Z53 and Z60. Due to different genetic background, it was impossible to recommend the B21 and Z67 genotypes as mother’s trees. The specific molecular keys were verified on 39 walnut mother's trees and the results were confirmed at two independent laboratories. The reported specific molecular keys can be used for identification of 5 Iranian walnut cultivars in juvenile period.

Keywords

Walnut; Microsatellite; Fingerprinting; Model based method; Clustering

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