Effect of different levels of drought stress and growth regulators on yield and yield components of common bean (Phaseolus vulgaris L.)

پژوهش‌های حبوبات ایران

Article 5, Volume 10, Issue 1, June 2019, Pages 63-74   

Document Type: Original Articles

DOI: 10.22067/ijpr.v10i1.62772

Authors

Siavosh Danir¹; Soran Sharafi¹; Esmaeel Gholinezhad^{* 2}

¹Department of Agronomy and Plant Breeding, Agriculture Faculty, Islamic Azad University of Mahabad, Mahabad, Iran

²Department of Agriculture Sciences, Payame Noor University, Tehran, Iran

Abstract

Introduction
Legumes have great nutritional value and are an important source of vegetable protein. Population growth in recent decades has led to increased consumption of protein, especially red meat. The increase in cereal production as supplemental protein sources is considered the country's economic development program. Legumes grain have 18 to 32 percent protein and plays an important role in people's diet. Drought stress is among the main limiting factors for legumes production, and is able to reduce significantly the production of grains, in rainfed areas. Stress inhibits the full expression of the genetic potential of crops and thereby reducing the production. Salicylic acid (SA), a plant phenolic compound is known as a plant hormone and its role in relation to defense mechanisms against biotic and abiotic stresses, is well known. Priming seed is a technique which the seeds before dealing with the ecological condition, will gain ability for better germination in terms of physiological and biochemical functioning. The aim of this study was to investigate the effect of spraying and priming of beans with salicylic acid on common bean yield and its components. It was also tried to determine whether priming or spraying with salicylic acid or both of them can induce tolerance drought stress to or not.
 
Materials & Methods
This experiment was performed at Research Station of Agricultural School, Urmia, Iran, during 2015-2016 growing season. The experiment was conducted as split-plot arrangement based on randomized complete block design with three replications. Treatments consisted of three levels of drought stress including severe drought stress (irrigation after 120 mm evaporation by class A pan), moderate drought stress (irrigation after 90 mm evaporation by class A pan), and control (irrigation after 60 mm evaporation by class A pan) as the main factor and treatments in five levels (control, priming with distilled water, priming with salicylic acid, spraying with salicylic acid, spraying and priming with salicylic acid) as subplots. Each experiment consisted of three replication with 5 plots. The first treatment control: conventional planting seeds. Second treatment was primed seeds of beans with distilled water. The seeds were primed for 12 hours in distilled water at room temperature. The third treatment: was seed priming with salicylic acid, so that the seeds for 12 hours in a solution of 0.5 micromoles salicylic acid was used at room temperature. The fourth plot: spraying the plants with a solution of 1 micromoles of salicylic acid on the field when the plants produced about 50 percent pod. Fifth treatment (v) was a combined treatment, priming the seeds before planting (seed for 12 hours in a solution of 0.5 micromoles of salicylic acid at 25⁰C temperature) and spraying the plants of the plot with a solution of 1 micromoles of salicylic acid on the field at 50 percent poding. After physiological maturity of beans, the crop was harvested and biomass, grain yield, 100 grain weight, the number of pods per plant, the number of grains per pods and plant height were measured. The data were analyzed with Tukey’s test at 95 percent probability (P≤0.05) using SAS and MSTATC statistical software.
 
 
Results & Discussion
Analysis of variance showed that grain yield, biological yield, index harvest, the number of pods per plant, the number of grains per pod, pod and seed weight, 100 grain weight and plant height were significantly affected by drought stress and growth regulators. Mean comparison showed that the maximum and minimum of traits were obtained under control (without stress) and severe drought stress, respectively. The highest and lowest values of studied traits were obtained by spraying and priming with salicylic acid and control (without priming). The highest (3362 kg ha^-1) and lowest (1864 kg ha^-1) grain yield were obtained under control (without stress) and severe drought stress, respectively. Drought stress in comparison with optimum irrigation (control) reduced grain yield by 45 percent. Treatment spraying and priming with salicylic acid in comparison with control (without priming) increased grain yield about 50 percent.
 
Conclusion
According to this research, under Urmia climate conditions, for producing high grain yield of common bean, using spraying and priming with salicylic acid can be recommended.

Keywords

Bean; Priming; Salicylic acid; Spraying; Water deficit

References

1. Abdel-Wahed, M.S.A., Amin, A.A., and Rashed, M. 2006. Physiological effect of some chemical constituents of yellow maize plants. World Journal Agricultural Sciences 2(2): 149-155.
2. Agha Alikhani, M., Tahmasebi Sarvestani, Z., and Ghafari Khaligh, H. 2002. The effect of water deficit stress on yield and yield components of three genotypes of Bean (Phaseolus vulgaris). (Abstract). In: Abstract Book of the 7rd Iranian Crop Science Congress. August 2002. Karaj Seed and Plant Improvement Institute. p. 541. (In Persian).
3. Amira, M., Hegazi, D., Amal, M., and El-Shraiy, E. 2007. Impact of salicylic acid and paclobutrazol exogenous application on the growth, yield and nodule formation of common Bean. Basic and Applied Sciences 1(4): 834-840.
4. Azhdar Afshari, M., Shekari, F., Afsahi, K., and Azimkhani, R. 2016. The effect of leaf application of Salicylic acid on dry weight, index harvest, yield and yield components of Bean (Phaseolus vulgaris) under water deficit stress. Environmental Stress in Crop Science 9(1): 51-58. (In Persian).
5. Bayat, A.A. 2008. The effect of moisture stress on yield and yield components of new cultivars Bean in Khomein. MSc. Thesis Faculty of Agriculture, Hamadan University. (In Persian).
6. Behboudian, M.H.M, Turner, N.C., and Palta, J.A. 2001. Reactions of chickpea to water stress: yield and seed composition. Journal of Science of Food and Agriculture 81: 1288-
7. Boutraa, T., and Sanders, F.E. 2001. Influence of water stress on grain yield and vegetative growth of two cultivars of Bean (Phaseolus vulgaris). Journal of Agronomy and Crop Science 187: 251-257.
8. Duman, I. 2006. Effect of seed priming with PEG and K₃PO₄on germination and seedling growth in Lettuce. Pakistan Journal of Biology Science 9(5): 923-928.
9. El-Tayeb, M.A. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation 45: 215-225.
10. Emadi, N., Balouchi, H.R., and Jahanbin, Sh. 2012. Effect of drought stress and plant density on yield, yield components and some morphological characters of pinto bean in Yasouj region. Electronic Journal of Crop Production 5(2): 1-17. (In Persian with English Summary).
11. Eraslan, F., Inal, A., Gunes, A., and Alpaslan, M. 2007. Impact of exogenous salicylic acid on growth, antioxidant activity and physiology of carrot plants subjected to combined salinity and boron toxicity. Scientia Horticulturae 113: 120-
12. 2014. Statistics. www.fao.org/stat.
13. Fariduddin, Q., Hayat, S., and Ahmad, A. 2003. Salicylic acid influences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity and see yield in Brassica Photosynthetica 41: 281-284.
14. Gholinezhad, E., Aeenehband, A., Hasanzade Ghorttappe, A., Barnoosi, I., and Rezaei, H. 2009. Evaluation of effective drought stress on yield, yield components and harvest index of sunflower hybrid Iroflor at different levels of nitrogen and plant population in Urmieh climate conditions. Journal of Plant Production 16(3): 1-28. (In Persian with English Summary).
15. Harper, J.P., and Balke, N.E. 1981. Characterization of the inhibition of K+ absorption in wheat roots by salicylic acid. Plant Physiology 68: 1349-1353.
16. Hayat, S., and Ahmad, A. 2007. Salicylic Acid: Plant H Springer. p. 97-99.
17. Khan, W., Prithviraj, B., and Smith, D.L. 2003. Photosynthetic responses of corn and soybean to foliar application of salicylates. Plant Physiology 160: 485-492.
18. Kocheki, A., and Banayan Aval, M. 1995. Pulse Crops. Jahad University of Mashhad. P. 236.
19. Kumar, H. 2000. Development potential of safflower in comparison to sunflower, sesame and safflower Newsletter. Institute of Sustainable A Spain 15: 86-89.
20. Lee, S.S., Kim, J.H., Hong, S.B., Yuu, S.H., and Park, E.H. 1998. Priming effect of rice seeds on seedling establishment under adverse soil conditions. Korean Journal of Crop Science 43: 194-
21. Madah, S.M., Falahian, F., Sabaghpour, H., and Chalbian, F. 2007. Effect of salicylic acid on grain yield and its components and anatomical structure of pea plants (Cicer arietinum). Journal of Basic Sciences of Islamic Azad University 1(62): 61-70. (In Persian).
22. Majnoon Hoseini, N. 2009. Grain Legume Production. Jehad University of Tehran. P. 294.
23. Mehrabian Moghaddam, N., Arvin, M.J., Khajoyinezhad, Gh., and Maghsodi, K. 2012. Effect of salicylic acid on growth, grain and forage yield of maize in drought stress conditions in farm. Seed and Plant Improvement Journal 27(2): 41-55. (In Persian).
24. Miar Sadegi, S., Shekari, F., Fotovat, R., and Zangani, E. 2011. The effect of priming by salicylic acid on vigor and seedling growth of canola (Brassica napus) under water deficit condition. Journal of Plant Biology 6: 55-70. (In Persian with English Abstract).
25. Mohammadi, G.R., and Amiri, F. 2010. The effect of priming on seed performance of canola (Brasica napus ) under drought stress. American-Eurasian Journal of Agriculture and Environment Science 9(2): 202-207.
26. Mohammadi, M., Fahimi, H., and Majd, A. 2010. The comparative effects of Salicylic acid and Gibberellin on seed germination of the Lentil (Lens culinaris). Journal of Biology of Islamic Azad University Branch of Garmsar 4(4): 9-12. (In Persian with English Summary).
27. Mohammadzadeh, A., Majnoon Hoseini, N., Moghaddam, H., and Akbari, M. 2013. Effect of different levels of drought stress and nitrogen on yield and yield components of two genotypes of Bean. Iranian Journal of Field Crop Science 43(1): 29-38. (In Persian with English Summary).
28. Noreen, Z., Ashraf, M., and Akram, N.A. 2010. Salt-induced regulation of some key antioxidant enzymes and physiobiochemical phenomena in five diverse cultivars of turnip (Brassica rapa). Journal of Agronomy and Crop Science 196: 273-285.
29. Pakmehr, A. 2009. Effect of priming by salicylic acid on morphological and physiological traits of cowpea (Vigna unguiculata) under water deficit. MSc. Thesis Faculty of Agriculture, Zanjan University. (In Persian).
30. Pakmehr, A., Shekari, F., and Rastgoo, M. 2014. Effect of seed priming by salicylic acid on some photosynthetic traits of cowpea under water deficit in flowering stage. Iranian Journal of Pulses Research 5(2): 19-30. (In Persian with English Summary).
31. Parsa, M. and Bagheri, A. 2008. Pulses. Jihad-e Daneshgahi Mashhad Publisher.
32. Rajabi, L., Sajedi, N.A., and Roshandel, M. 2013. Response of yield and yield component of dry land chickpea to salicylic acid and superabsorbent polimer. Journal of Crop Production Research 4(4): 343-353. (In Persian with English Summary).
33. Rezaei Chiyaneh, E., and Pirzad, A.R. 2014. The effect of salicylic acid on yield, yield components and essence of (Nigella sativa) in condition of water deficit stress. Iranian Journal of Field Crops Research 12(3): 427-437. (In Persian).
34. Rosales-Serena, R., Kohashi-Shibata, J., Trejo- Lopez, A., Ortiza-Cereceres, C., and Kelly, J.D. 2002. Yield and phonological adjustment in four drought-stressed common bean cultivars. Annual Red Bean Improvement Crop 45: 198-199.
35. Sabzi, S., Tahmasebi, Z., and Barari, M. 2017. The effect of irrigation different levels effect on grain yield and some traits of Bean (Phaseolus vulgaris) genotypes. Environmental Stress in Crop Science 10(1): 21-30. (In Persian).
36. Sadat Rasti Sani, M., Lahouti, M., and Ganjeali, A. 2014. Effect of drought stress on some morphophysiological traits and chlorophyll fluorescence of red bean seedlings (Phaseolus vulgaris). Iranian Journal of Pulses Research 5(1): 103-116. (In Persian with English Summary).
37. Senaratna, T., Touchell, D., Bunn, E., and Dixon, K. 2003. Acetyl salicylic acid (Asprin( and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation 30: 157-161.
38. Sepehri, A., Abasi, R., and Karami, A. 2016. Effect of drought stress and salicylic acid on grain yield and its component of genotypes of bean (Phaseolus vulgaris). Journal of Crop Improvement 17(2): 503-513. (In Persian).
39. Shakirova, F.M., and Sahabutdinova, D.R. 2003. Change in the hormonal status of wheat seedling induced by salicylic acid and salinity. Plant Science 164: 317-322.
40. Shekari, F., Pakmehr, A., Rastgho, M., Vazayefi, M., and Ghoreyshinasab, M.J. 2011. Effect of salicylic acid seed priming on physiological traits bean (Vigna unguiculata) under water stress in pod time. Agricultural Sciences Journal of Islamic Azad university of Tabriz 4(13): 13-29. (In Persian).
41. Singh, B., and Usha, K. 2003. Salicylic acid induced physiological changes in wheat seedlings under water stress. Plant Growth Regulation 39: 137-141.
42. Singh, S.H. 2003. Drought resistance in the Race Durango dry bean landraces and c Agronomy Journal 99: 1919-1225.
43. Sio-Se Mardeh, A., Sadeghi, F., Kanouni, H., Bahramnejad, , and Gholami, S. 2014. Effect of drought stress on physiological traits, grain yield and its components in chickpea (Cicer arietinum L.) genotypes. Iranian Journal of Crop Sciences 16(2): 91-108. (In Persian with English Summary).
44. Soheili Movahed, S., Esmaeili, M.A., Jabbari, F., and Foladi, A. 2017. Evaluation yield and its component some of bean genotypes in water limitation condition of season end. Agroecology Journal 9(4): 1-12.
45. Soleimani, M.R., Kafi, M., Ziaee, M., and Shabahang, J. 2009. Effect of limited irrigation with saline water on forage of two local populations of Kochia scoparia Schrad. Water and Soil 22(2): 307-317.
46. Washem, M. 2006. Influence of Exogenously Applied Salicylic Acid on Drought Tolerance of Hexaploid W Botany Department of Bitany University of Agriculture Faisalabad. Ph.D. Dissertation.
47. Zadeh Bagheri, M., Javanmardi, Sh., Alozadeh, O., and Kamelmanesh, M.M. 2015. Effects of drought on grain yield and some physiological characteristics of red bean genotypes. Plant Ecophysiology 6(18): 1-11. (In Persian with English Summary).
48. Zarshin Zenosh, R., Ansari, M.H., and Mostafavi Rad, M. 2015. The effect of chemical and biological priming on yield and yield components of Broad Bean (Vicia faba). Plant Environmental Physiology 10(40): 73-83. (In Persian with English Abstract).
49. Zabet, M., and Hoseinzadeh, A. 2011. Determine the most important traits affecting Vadiata wilczekvigna yield using multivariate statistical methods in condition of drought stress and non-stress. Iranian Journal of Pulses Research 2(1): 87-98. (In Persian with English Summary).
50. Zhu, J.K. 2002. Salt and drought stress signal transduction in plants. Annual Review of Plant Biology 53: 247-316.