Effects of Organic amendments and Biofertilizer Application on some Morphological Traits and Yield of hyssop (Hyssopus officinalis L.)

علوم باغبانی

Article 7, Volume 30, Issue 3, September 2016, Pages 491-500   

Document Type: Research Article

DOI: 10.22067/jhorts4.v30i3.46003

Authors

Mohammad Taghi Darzi^* ; Bijan Sadeghi Nekoo

Islamic Azad University, Roudehen Branch

Abstract

Introduction: Hyssop (Hyssopus officinalis) is a perennial herb which is cultivated in temperate regions of Asia, Europe and America. The essential oil of hyssop is widely used as traditional drug in some of the parts of Asia and Europe to treat respiratory diseases. Hyssop is also used in food, pharmaceutical, flavor and cosmetic industries throughout the world. It is mainly used for antispasmodic, stomachic, antifungal, relax spasm and cough treatment. Applying organic fertilizers and biofertilizers such as compost, vermicompost and nitrogen fixing bacteria contain Azotobacter and Azospirillum has led to a decrease in chemical fertilizers consumption and has provided high quality agricultural products. Several studies have shown that organic and bio-fertilizers application such as vermicompost and nitrogen fixing bacteria can increase essential oil content of medicinal plants of dill, basil and fennel.The main object of this work was to record the best suitable treatment of organic and biological fertilizer to obtain highest yield of Hyssop.
Materials and Methods: A field experiment, arranged in a randomized complete blocks design with eight treatments and three replications at research field of the Agriculture Company of Ran, Firouzkuh, Iran during the growing season of 2012. The treatments were (1) 20 t/ha compost, (2) 12 t/ha vermicompost, (3) biofertilizer [inoculated seeds with Azotobacter and Azospirillum], (4) 10 t/ha compost + 6 t/ha vermicompost, (5) 20 t/ha compost + biofertilizer, (6) 12 t/ha vermicompost + biofertilizer, (7) 10 t/ha compost + 6 t/ha vermicompost + biofertilizer and (8) control [without fertilizer application]. Inoculation was carried out by immersing the hyssop seeds in the cells suspension of 108 CFU/ml for 15 min. The required quantities of compost and vermicompost were applied and incorporated to the top 5 cm layer of soil in the experimental beds before planting of hyssop seeds. Each experimental plot was 3 m long by 2.28 m wide with the spacing of 15 cm between the plants and 38 cm between the rows. There was a space of one meter between the plots and 2 meters between replications. Hyssop seeds were directly sown by hand. There was no incidence of pest or disease on hyssop during the experiment. Weeding was done manually and the plots were irrigated weekly (as trickle irrigation system). All necessary cultural practices and plant protection measures were followed uniformly for all the plots during the entire period of experiment. In this study, some morphological traits and yield hyssop including plant height, branch no./plant, fresh weight of plant, dry weight of plant, herb fresh yield, herb dried yield, essential oil content and essential oil yield were evaluated. In order to determine herb fresh yield and herb dry yield, 1 m-2 plants were used at the harvest time (full blooming). For determine the essential oil content (%), About 100 g dried herb of hyssop (dried in shadow) as sample from the each plot were selected and then were subjected to hydro-distillation (Clevenger type apparatus) for 3 hours. Essential oil yield also was calculated by using essential oil content and herb dried yield. Analysis of variance by using SAS software and mean comparisons by Least Significant Difference Test (LSD) (at the 5% probability level) was done.
Results and Discussion: The present results have shown that the highest plant height and branch no/plant in treatment of integrated application of 20 t/ha compost,biofertilizer,the maximum fresh weight of plant and herb fresh yield in treatment of application of 20 t/ha compost were obtained. Also, the highest dry weight of plant in treatment of integrated application of 10 t/ha compost, 6 t/ha vermicompost and biofertilizer and the highest herb dried yield, essential oil content and essential oil yield in treatment of integrated application of 12 t/ha vermicompost and biofertilizer were obtained. Vermicompost application through high ability for absorption of mineral nutrients like N, P and K by plants and then increase of biomass amount has a positive effect on secondary metabolites concentration such as essential oil and subsequently the enhanced essential oil content. Increased essential oil yield in treatment of 12 tonnes vermicompost per hectare and biofertilizer can be owing to the improvement of yield attributes such as herb dry yield and essential oil content.
Conclusion:Integrated application of organic manures and biofertilizer positively influenced morphological traits and yield of hyssop, as the highest herb dry yield, essential oil content and essential oil yield were obtained in treatment of integrated application of 12 t/ha vermicompost and biofertilizer and treatment of integrated application of 10 t/ha compost, 6 t/ha vermicompost and biofertilizer. On the base of research results, organic amendments and biofertilizer application can be caused in improvement of morphological characters and yield of hyssop.

Keywords

Azospirillum; Azotobacter; Compost; Essential oil; Vermicompost

References

1. Abdou M.A.H., El-Sayed A.A., Badran F.S. and El-Deen R.M.S. 2004. Effect of planting density and chemical and biofertilization on vegetative growth, yield and chemical composition of fennel (Foeniculum vulgare Miller): I-Effect of planting density and some chemical (Nofatrein) and biochemical (Biogen) fertilizers, Annals of Agricultural Science, Moshtohor, 42: 1907-1922.

2. Anwar M., Patra D.D., Chand S., Alpesh K., Naqvi A.A. and Khanuja S.P.S. 2005. Effect of organic manures and inorganic fertilizer on growth, herb and oil yield, nutrient accumulation, and oil quality of French basil, Communications in Soil Science and Plant Analys, 36: 1737-1746.

3. Arancon N., Edwards C.A., Bierman P., Welch C. and Metzger J.D. 2004. Influences of vermicomposts on field strawberries: 1. Effects on growth and yields, Bioresource Technology, 93: 145-153.

4. Ateia E.M., Osman Y.A.H. and Meawad A.E.A.H. 2009. Effect of organic fertilization on yield and active constituents of Thymus vulgaris L. under North Sinai conditions, Research Journal of Agriculture and Biological Sciences, 5: 555-565.

5. Badran F.S. and Safwat M.S. 2004. Response of fennel plants to organic manure and bio-fertilizers in replacement of chemical fertilization, Egyptian Journal of Agricultural Research, 82: 247-256.

6. Darzi M.T. and Haj Seyed Hadi M.R. 2012a. Effects of the application of organic manure and biofertilizer on the fruit yield and yield components in dill (Anethum graveolens L.), Journal of Medicinal Plants Research, 6: 3266-3271.

7. Darzi M.T., Haj Seyed Hadi M.R. and Rejali F. 2012b. Effects of the application of vermicompost and nitrogen fixing bacteria on quantity and quality of the essential oil in dill (Anethum graveolens L.), Iranian Journal of Medicinal and Aromatic Plants, 6: 3793-3799.

8. Darzi M.T., Haj Seyed Hadi M.R. and Rejali F. 2012c. Effects of Cattle Manure and Biofertilizer Application on Biological Yield, Seed Yield and Essential oil in Coriander (Coriandrum sativum L.), Journal of Medicinal Plants, 11: 77-90.

9. El Ghadban E.A.E., Shalan M.N. and Abdel L.T.A.T. 2006. Influence of biofertilizers on growth, volatile oil yieldand constituents of fennel (Foeniculum vulgare Mill.), Egyptian Journal of Agricultural Research, 84: 977-992.

10. Fallahi J., Koocheki A. and Rezvani Moghaddam P. 2009. Investigating the effects of organic fertilizer on quantity index and the amount essential oil and chamazulene in chamomile (Matricaria recutita L.), Agricultural Research: Water, Soil and Plant in Agriculture, 8: 157-168.

11. Kapoor R., Giri B. and Mukerji K.G. 2004. Improved growth and essential oil yield and quality in Foeniculum vulgare Mill on mycorrhizal inoculation supplemented with P-fertilizer, Bioresource Technology, 93: 307-311.

12. Kizil S., Hasimi N., Tolan V., Kilinc E. and Karatas H. 2010. Chemical Composition, Antimicrobial and Antioxidant Activities of Hyssop (Hyssopus officinalis L.) Essential Oil, Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38: 99-103.

13. Koocheki A., Tabrizi L. and Ghorbani R. 2009. Effect of biofertilizers on agronomic and quality criteria of Hyssop (Hyssopus officinalis L.), Iranian Journal of Agronony Research, 6: 127-137.

14. Loomis W.D. and Corteau R. 1972. Essential oil biosynthesis. Recently Advance Phytochem, 6: 147-185.

15. Mahfouz S.A. and Sharaf Eldin M.A. 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill), International Agrophisics, 21: 361-366.

16. Makkizadeh M., Nasrollahzadeh S., Zehtab Salmasi S., Chaichi M. and Khavazi K. 2012. The Effect of Organic, Biologic and Chemical Fertilizers on Quantitative and Qualitative Characteristics of Sweet Basil (Ocimum basilicum L.), Journal of Agriculture Science and Sustainable Production, 22: 1-12.

17. Migahed H.A., Ahmed A.E. and Abdel Ghany B.F. 2004. Effect of different bacterial strains as biofertilizer agents on growth, production and oil of Apium graveolens under calcareous soil, Arab Universities Journal of Agricultural Science, 12: 511-525.

18. Moradi R., Rezvani M.P., Nasiri M.M., Lakzian A. 2010. The effect of application of organic and biological fertilizers on yield, yield components and essential oil of Foeniculum vulgare (Fennel), Iranian Journal of Agronomy Research, 7: 625-635.

19. Moro A., Zalacain A., De Mendoza J.H. and Carmona M. 2011. Effects of Agronomic Practices on Volatile Composition of Hyssopus officinalis L. Essential Oils, Molecules, 16: 4131-4139.

20. Mottaghian A., Pirdashti H., Bahmanyar M.A. and Mottaghian B. 2013. Response of growth characteristics and nutrients uptake of basil (Ocimum basiilicum L.) to concomitant use of municipal waste compost and three species of Trichoderma, Iranian Journal of Medicinal and Aromatic Plants, 29: 358-372.

21. Omidbaigi R. 2000. Production and processing of medicinal plants, Volume 3, Behnashr Co. 397 p.

22. Rahimzadeh S., Sohrabi Y., Heidary G.R. and Pirzad A.R. 2011. Effect of biofertilizers application on some morphological traits and yield of dragonhead (Dracocephalum moldavica L.), Journal of Horticultural Science, 25: 335-343.

23. Saeid Nejad A.H. and Rezvani Moghaddam P. 2010. Evaluation of biofertilizer and chemical fertilizer application on morphological traits, yield, yield components and essential oil percent in cumin (Cuminum cyminum L.), Journal of Horticultural Science, 24: 38-44.

24. Salehi Surmeghi M.H. 2008. Medicinal plants and Phytotherapy, Volume 2, Doniaye Taghzieh. 377 p.

25. Sefidkon F. 2001. Evaluation of qualitative and quantitative essential oil fennel (Foeniculum vulgare Mill) in different stages of growth, Iranian Journal of Medicinal and Aromatic Plants, 7: 85-104.

26. Sharma A.K. 2002. Biofertilizers for sustainable agriculture, Agrobios, India, 407 p.

27. Tahami S.M.K., Rezvani Moghaddam P. and Jahan M. 2010. Comparison the effect of organic and chemical fertilizers on yield and essential oil percentage of Basil (Ocimum basilicum L.), Journal of Agroecology, 2: 70-82.

28. Yousefzadeh S., Modarres Sanavy S.A.M., Sefidkon F., Asgarzadeh A., Ghalavand A., Roshdi M. and Safaralizadeh A. 2013. Effect of biofertilizer, azocompost and nitrogen on morphologic traits and essential oil content of Dracocephalum moldavica L. in two regions of Iran, Iranian Journal of Medicinal and Aromatic Plants, 29: 438-459.