بررسی ثبات واحدهای SSR پلاسمید pEA29 جدایه‌های عامل بیماری سوختگی آتشی

پژوهش های حفاظت گیاهان ایران

مقاله 10، دوره 29، شماره 3 - شماره پیاپی 32، مهر 1394، صفحه 459-465   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v29i3.44770

نویسندگان

ساره بقائی راوری^* ؛ الهام جمشیدی

دانشگاه فردوسی مشهد

چکیده

این تحقیق با هدف بررسی ردیف‌های تکراری کوتاه (SSR) در ناحیه PstI پلاسمید pEA29 جدایه‌های amylovora Erwinia جمع‌آوری شده از نواحی مختلف کشور و ارزیابی پایداری آن‌ها در شرایط آزمایشگاه انجام گرفته است. آزمون‌های فنوتیپی و بیماریزایی تنوعی را در جدایه‌های مورد بررسی نشان ندادند. وجود پلاسمید pEA29 با استفاده از PCR آشیانه‌ای تایید و تکثیر و توالی یابی بخشی از ناحیه PstI حاوی واحدهای SSR برای تمام جدایه‌ها انجام گرفت. تعداد واحدهای تکراری برای جدایه‌های سیب و رز به ترتیب 4 و 8 عدد و برای جدایه‌های به، رز و زالزالک بین 4، 5 و 7 متغیر بودند. ثبات واحدهای SSR تحت سه تیمار تکثیر طولانی مدت، نگهداری جدایه‌ها در شرایط خنک و جداسازی مجدد از بافت‌های آلوده ارزیابی گردید و به جز جدایه IrGh59 تحت تیمار نگهداری طولانی مدت در سرما، در بقیه جدایه‌ها تغییری مشاهده نشد. بر اساس نتایج این تحقیق، تعداد واحدهای SSR به دلیل دارا بودن ثبات کافی در شرایط آزمایشگاهی، برای گروه‌بندی جدایه‌های عامل سوختگی آتشی قابل استفاده می‌باشند.

کلیدواژه‌ها

ایران؛ پلاسمید pEA29؛ ثبات واحدهای تکراری؛ گروه‌بندی

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