مطالعه شبکه ژنی آنژیوژنز در جوجه‌های مرغ آرین حساس به آسیت با استفاده از داده‌های RNA-Seq

پژوهشهای علوم دامی ایران

مقاله 6، دوره 10، شماره 2 - شماره پیاپی 34، تیر 1397، صفحه 249-262   

نوع مقاله: علمی پژوهشی- ژنتیک و اصلاح دام و طیور

شناسه دیجیتال (DOI): 10.22067/ijasr.v10i2.65215

نویسندگان

وحید تقی زاده^{* 1}؛ محمدرضا نصیری²؛ مجتبی طهمورث پور³؛ محمدرضا بختیاری زاده⁴؛ علی جوادمنش³

¹پردیس واحد بین‌الملل دانشگاه فردوسی مشهد

²گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

³گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران.

⁴گروه علوم دام و طیور، پردیس ابوریحان، دانشگاه تهران، تهران، ایران

چکیده

سندروم آسیت یک عارضه متابولیکی است که در سنین بالا در پرندگان گوشتی بروز می‌کند و به سویه تجاری آرین که تنها لاین اصلاح شده مرغ گوشتی ایران می باشد آسیب جدی وارد کرده است. مطالعات گذشته نقش عوامل ژنتیکی در بروز این سندروم را اثبات کرده است. از آنجا که ارگان‌های متعددی در بروز آسیت دخالت دارند و نقش قلب در آنها از همه بارزتر است، از مطالعات ژنومی استفاده شد تا ژن‌های مرتبط با مسیر آنژیوژنز در هایپرتروفی بطن راست قلب شناسایی شوند. در این تحقیق تعداد 464 پرنده از خط پدری B لاین آرین تحت تنش سرمایی پرورش داده شد و در بررسی آنالیز داده هایRNA-seq بین دو گروه سالم و مبتلا به آسیت، پروفایل بیان ژن‌ها در بطن راست قلب مرغان حساس و مقاوم به آسیت با استفاده از تکنولوژی توالی‌یابی نسل آینده مورد مقایسه قرار گرفت. نتایج بررسی ترنسکریپتومی ژنهای مسیر آنژیوژنز نشان داد که تعداد 59 ژن بین دو تیمار سالم و آسیتی تفاوت بیان معنی‌دار داشتند )05/0P

کلیدواژه‌ها

آسیت؛ آنالیز مسیر؛ آنژیوژنز؛ شبکه ژنی؛ هستی شناسی ژن؛ RNA-seq

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