امکان القای مقاومت در گوجه فرنگی علیه Rhizoctonia solani و برخی مکانیسم های آن

پژوهش های حفاظت گیاهان ایران

مقاله 3، دوره 30، شماره 3 - شماره پیاپی 33، مهر 1395، صفحه 441-448   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v30i3.44805

نویسندگان

فریبا نیک رفتار؛ پریسا طاهری^* ؛ سعید طریقی

دانشگاه فردوسی مشهد

چکیده

به منظور بررسی امکان القای مقاومت در گیاه گوجه فرنگی رقم حساس موبیل علیه قارچ Rhizoctonia solani، از ویتامین ها و هموسرین لاکتون ها استفاده شد. تیمار تیامین 20 میلی مولار بهترین تأثیر را در القای مقاومت علیه این بیمارگر داشت. جهت بررسی نقش پراکسیداز در مقاومت القایی ناشی از تیامین، از سدیم آزید به عنوان بازدارنده پراکسیداز استفاده شد و در زمان های 0، 24، 48 و 72 ساعت بعد از مایه زنی قارچ، میزان ترکیبات فنلی در نمونه های تیمار شده و شاهد اندازه گیری شد. بین محتوای فنل کل در نمونه های مختلف و زمان های متفاوت مورد بررسی، تفاوت قابل توجهی مشاهده شد. در نمونه های تیمار شده با سدیم آزید میزان ترکیبات فنلی نسبت به دیگر نمونه ها کمتر بود. جلوگیری از فعالیت این آنزیم منجر به کاهش تولید ترکیبات فنلی و کاهش میزان مقاومت به بیمارگر در برگ های تیمار شده با تیامین و سدیم آزید گردید. بر اساس نتایج این تحقیق، پراکسیداز و ترکیبات فنلی نقش مهمی در مقاومت القایی ناشی از تیامین در گوجه فرنگی علیه R. solani ایفا می کنند.

کلیدواژه‌ها

القای مقاومت؛ پراکسیداز؛ گوجه فرنگی؛ محتوای فنل کل؛ ویتامین B1

مراجع

1- Abdel- Monaim M.F. 2011. Role of riboflavin and thiamine in induced resistance against charcoal rot disease of soybean. African Journal of Biotechnology , 10: 10842-10855.

2- Ahn I. P., Kim S., and Lee Y.H. 2005. Vitamin B1 functions as an activator of plant disease resistance. Journal of Plant Physiology, 138:1505-1515.

3- Ahn I.P., Kim S., Lee, W.H., and Suh S.C. 2007. Vitamin B1-induced priming is dependent on hydrogen peroxide and the NPR1 gene in Arabidopsis. Journal of Plant Physiology, 143: 838–848.

4- Bahuguna R.N., Joshi R., Shukla A., Pandey M., and Kumar J. 2012. Thiamine primed defense provides reliable alternative to systemic fungicide carbendazim against sheath blight disease in rice (Oryza sativa L.). Plant Physiology and Biochemistry, 57: 159-167.

5- Blokhina O.B., Chirkova T.V., and Fagerstedt K.V. 2001. Anoxic stress leads to hydrogen peroxide formation in plant cells. Journal of Experimental Botany, 52, 1179–90

6- Boubakri H., Wahab M.A., Chong J., Bertsch C., Mliki A., and Gacougnolle I.S. 2012. Thiamine induced resistance to Plasmopara viticola in grapevine and elicited host defense responses, including HR like-cell death. Plant Physiology and Biochemistry, 57: 120-13.

7- Denslow Sh.A., Rueschhoff E.E., and Daub M.E. 2007. Regulation of the Arabidopsis thaliana vitamin B6 biosynthesis genes by abiotic stress. Plant Physiology and Biochemistry, 45: 152-161.

8- Djebali N., Mhadhbi H., Lafitte C., Dumas B., Esquerre-Tugaye M. T., Aouani M. E., and Jacquet C. 2011. Hydrogen peroxide scavenging mechanisms are componentsof Medicago truncatula partial resistanceto Aphanomyces euteiches. European Journal of Plant Pathology, 131: 559-571.

9- Gonzalez G., Portal Onco M.A., and Rubio SusanV. 2006. Biology and systematic of form genus Rhizoctonia. Spanish Journal of Agricultural Research, 4: 55-79.

10- Goyer A. 2010. Thiamine in plants: aspects of its metabolism and functions, Phytochemistry, 17: 1615–1624.

11- Harrison S., Curtis M., McIntyre C., Maclean D., and Manners J. 1995. Differential expression of peroxidase isogenes during the early stages of infection of the tropical forage legume Stylosanthes humilis by Colletotrichum gloeosporioides. Molecular Plant-Microbe Interaction, 8: 398-406.

12- Hung K.T., and Kao C.H. 2004. Hydrogen peroxide is necessary for abscisic acid-induced senescence of rice leaves. Journal of Plant Physiology, 161: 1347-1357.

13- Hilaire E., Young S.A., Willard L.H., McGee J.D., Sweat T., Chittoor J.M., Guikema J.A., and Leach J.E. 2001. Vascular defense responses in rice: Peroxidase accumulation in xylem parenchyma cells and xylem wall thickening. Molecular Plant-Microbe Interaction. 14: 1411-1419.

14- Jung W.J., Mabood F., Kim T.H., and Smith D.L. 2007. Induction of pathogenesis-related proteins during biocontrol of Rhizoctonia solani with Pseudomonas aureofaciens in soybean (Glycine max L. Merr.) plants. BioControl, 52: 895–904.

15- Kuramae E.E., Buzeto A.L., Ciampi M.B., and Souza N.L. 2003. Identification of Rhizoctonia solani AG 1-IB in lettuce, AG 4 HG-I in tomato and melon, and AG 4 HG-III in broccoli and spinach, in Brazil. European Journal of Plant Pathology, 109: 391–395.

16- Luzzatto T., Golan A., Yishay M., Bilkis I., Ben-Ari J., and Yedidia I. 2007. Priming of antimicrobial phenolics during induced resistance response towards Pectobacterium carotovorumin the ornamenta lmonocot calla lily. Journal Agricultural and Food Chemistry, 55: 10315–10322.

17- Matern U., and Kneusal R.E. 1998. Phenolic compounds in plant disease resistance. Phytoparasitica, 16: 153–170.

18- Nikraftar F., Taheri P., Flahati-Rastgar M., and Tarighi S. 2013. Tomato partial resistance to Rhizoctonia solani involves antioxidative defense mechanisms. Physiological and Molecular Plant Pathology 81:74–83.

19- Oñate-Sanchez L., and Vicente-Carbajosa J. 2008. DNA-free RNA isolation protocols for Arabidopsis thaliana, including seeds and siliques. BMC Research Notes, 1: 1-7.

20- Pushpalatha H.G., Mythrashree S.R., Shetty R., Geetha N.P., Sharathchandra R.G., Amruthesh K.N., and Shetty H.S. 2007. Ability of vitamins to induce downy mildew disease resistance and growth promotion in pearl millet. Crop Protection, 26: 1674–1681.

21- Pushpalatha H.G., Sudisha J., Geetha N.P., Amruthesh K.N., and Shekar Shetty H. 2011. Thiamine seed treatment enhances LOX expression, promotes growth and induces downy mildew disease resistance in pearl millet. Biologia Plantarum, 55: 522-527.

22- Rai G.K., Kumar R., Singh J., Rai P.K., and Rai S.K. 2011. Peroxidase, polyphenol oxidase activity, protein profile and phenolic content in tomato cultivars tolerant and susceptible to Fusarium oxsyporum f.sp. lycopersici. Journal of Botany, 43: 2987-2990.

23- Rajasekaran K., Cary J.W., Jacks T.J., Stromberg K.D., and Cleveland T.E. 2000. Inhibition of fungal growth in planta and in vitro by transgenic tobacco expressing a bacterial nonheme chloroperoxidase gene. Plant Cell Reports, 19:333–338

24- Sadrovi M., and Setayeshmehr F. 2008. Fungal diseases of tomato in North Khorasan province and the reaction of four commercial cultivars to their Pathogens. Journal of plant diseases,44: 255- 261. (in Persian)

25- Schikora A., Schenk S.T., Stein E., Molitor A., Zuccaro A., and Kogel K.H. 2011. N-Acyl-homoserine lactone confers resistance toward biotrophic and hemibiotrophic pathogens via altered activation of AtMPK6. Plant Physiology, 157: 1407–1418.

26- Schuhegger R., Ihring A., Gantner S., Bahnweg G., Knappe C.,Vogg G., Hutzler P., Schmid M., Breusegem F.N., Eberl L., Hartmann A., and Langebartels C. 2006. Induction of systemic resistance in tomato by N-acyl-L-homoserine lactone-producing rhizosphere bacteria. Plant, Cell and Environment, 29: 909–918.

27- Schwartz H.F., and Gent D.H. 2007. Homepage of high plains integrated pest management. Damping off and seedling blight. wiki.bugwood.org, Visited: 2007/01/04

28- Seevers P.M., Daly J.M., and Catedral F.F. 1971. The Role of Peroxidase Isozymes in Resistance to Wheat Stem Rust Disease. Journal of Plant physiology, 48: 353-360.

29- Shahbazi H., Aminian H., Sahebani N., and Halterman D. A. 2010. Biochemical Evaluation of Resistance Responses of Potato to Different Isolates of Alternaria solani. Phytopathalogy, 100: 454-459.

30- Sneh B., Burpp L., and Ogoshi A. 1991. Identification of Rhizoctonia species. APS Press, 133 Pp.

31- Taheri P., Gnanamanickam S., and Hofte M. 2007. Characterization, genetic structure, and pathogenicity of Rhizoctonia spp. associated with rice sheath diseases. Phytopathology, 97: 373–83.

32- Taheri P., and Tarighi S. 2009. A study on the effect of riboflavin as a defense activator in rice against Rhizoctonia diseases. Journal of Plant Protection, 23: 68- 80.(in Persian)

33- Taheri P., and Tarighi S. 2010. Riboflavin induces resistance in rice against Rhizoctonia solani via jasmonate-mediated priming of phenylpropanoid pathway. Journal of Plant Physiology, 167: 201–208.

34- Taheri P., and Tarighi S. 2011. A survey on basal resistance and riboflavin-induced defense responses of sugar beet against Rhizoctonia solani. Journal of Plant Physiology, 168: 1114–1122.

35- Taheri P., and Tarighi S. 2012. The Role of Pathogenesis-Related Proteins in Tomato-Rhizoctonia solani Interaction. Journal of Botany, 2012. http://dx.doi.org/10.1155/2012/37037.