بررسی تأثیر سطوح مختلف گوگرد بر رشد، صفات کیفی و عملکرد گل گیاه دارویی آرنیکای چامیسو (Arnica chamissonis Less. ssp. foliosa)

علوم باغبانی

مقاله 5، دوره 33، شماره 2، شهریور 1398، صفحه 219-232   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jhorts4.v0i0.71858

نویسندگان

مژده اسدی¹؛ جواد هادیان^{* 1}؛ صمد نژاد ابراهیمی¹؛ قاسم کریم زاده²

¹دانشگاه شهید بهشتی تهران

²دانشگاه تربیت مدرس

چکیده

آرنیکای چامیسو با نام علمی Arnica chamissonis Less. ssp. foliosa گیاهی چندساله، ریزوم‌دار، با مصارف دارویی از خانواده آفتابگردان و بومی آمریکای شمالی و کانادا است. با توجه به نیاز این گیاه به مناطق خنک و خاک‌های با pH اسیدی و با هدف بررسی سازگاری این گیاه در ایران، تأثیر سطوح مختلف کود گوگرد بر کاهشpH  خاک و عملکرد آرنیکای چامیسو مورد بررسی قرار گرفت. آزمایش در قالب طرح بلوک کامل تصادفی با 4 تیمار شامل سطوح مختلف کود گوگرد بنتونیتی (صفر، 250، 500 و 750 کیلوگرم در هکتار) به همراه باکتری thiooxidans  Thiobacillusدر سه تکرار انجام شد. صفاتی از جمله ارتفاع، سطح مقطع بوته، تعداد گل در بوته، قطر گل‌آذین، عملکرد وزن ‌تر و خشک گل در بوته و در مترمربع، هم‌چنین محتوای فنل و فلاونوئید کل، روتین، لوتئولین و آپی­جنین عصاره گل­ها اندازه‌گیری شدند. نتایج این بررسی نشان داد که آرنیکای چامیسو با منطقه مورد پژوهش سازگاری داشته و می‌توان آن را در اقلیم‌های مشابه، در مقیاس وسیع کشت کرد. در گیاهان تغذیه شده با تیمار 250 کیلوگرم گوگرد در هکتار بیش‌ترین ارتفاع بوته ( 6/25 سانتی­متر)، سطح مقطع بوته ( 48/25 سانتی­متر)، قطر ساقه (49/3 میلی­متر)، قطر گل (61/29 میلی­متر)، تعداد کل گل در بوته (66/58) و عملکرد گل (67/20 گرم در بوته) نسبت به شاهد مشاهده شد. مصرف سطوح بالای گوگرد سبب کاهش صفات رشدی و عملکردی و افزایش فنل کل، فلاونوئید کل، روتین، لوتئولین و آپی­جنین عصاره گل­ها شد که این امر با افزایش قابل ملاحظه هدایت الکتریکی خاک همراه بود.

کلیدواژه‌ها

اصلاح خاک؛ تغذیه گیاه؛ سازگاری؛ کود گوگرد

مراجع

1- Akula, R. and Ravishankar, G. A. 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling and Behavior, 6: 1720-1731.

2- Bahreininejad, B., Razmjou, J., and Mirza, M. 2013. Influence of water stress on morpho-physiological and phytochemical traits in Thymus daenensis. International Journal of Plant Production, 7(1): 151-166.

3- Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Science Research, 14: 93-107.

4- Caliskan, O., Radusiene, J., Temizel, K.E., Staunis, Z., Cirak, C., Kurt, D., and Odabas, M.S. 2017. The effects of salt and drought stress on phenolic accumulation in greenhouse-grown Hypericum pruinatum. Italian Journal of Agronomy, 12(3): 271-275.

5- Dash, S. and Gupta, N. 2011. Microbial bioinoculants and their role in plant growth and development. International Journal of Biotechnology and Molecular Biology Research, 2: 232-251.

6- Dawood, F. A., Al-Omari, S. M., & Murtadha, N. S. 1985. High levels of sulfur affecting availability of some micronutrients in calcareous soils (in Iraq). J. of Agriculture and Water Resources Research (Iraq).‏

7- Delabays, N. and Mange, N. 1991. La culture d' Arnica montana L: aspects agronomiques et phytosanitaires. Revue suisse de viticulture, arboriculture, horticulture.

8- Doroudian, H.R., BesharatiKelayeh, H., FallahNosrat Abad, A.R., Heidary Sharif Abadi, H., Darvish, F., and Allahverdi, A. 2010. Study of absorbable phosphorus changes in lime soils and its impact on corn yield. Agricultural Modern Knowledge (Modern Knowledge of Sustainable Agriculture) 6(18): 27-35. (In Persian with English Summary).

9- El-Din, A.E., Aziz, E.E., Hendawy, S.F., and Omer, E.A. 2009. Response of Thymus vulgaris L. to salt stress and alar (B9) in newly reclaimed soil. Journal of Applied Sciences Research, 5: 2165-2170.

10- Esra, K.O.Ç., İŞLEK, C., and Üstün, A.S. 2010. Effect cold on protein, proline, phenolic compounds and chlorophyll content of two pepper (Capsicum annuum L.) varieties. Gazi University Journal of Science, 23(1): 1-6.

11- Finch C., Grant, G., Patersons, J., and Extension, B. C. 2004. Sulfur and soil pH, www.plantansewers.com, garden column.

12- Garcia-gomez, A., Bernal, M., and Roig, A. 2002. Growth of ornamental plants in two composts prepared from agroindustrial wastes. Bioresource Technology, 83: 81-87.

13- Gaspar, A., Cracinescu, O., Trif, M., Moisei, M., and Moldovan, L. 2014. Antioxidantand anti-inflammatory properties of active compounds from Arnica montana L. Romanian Biotechnological Letters 19 (3), 9353-9365.

14- Gawlik-Dziki, U.,´Swieca, M., Sugier, D., and Cichocka, J. 2009. Seeds of Arnica montana and Arnica chamissonis as a potential source of natural antioxidants. HerbaPolonica 55, 60-71.

15- Goodarzi, K. and Hosseini Farahi, M. 2014. Evaluating the ability of sulfur and animal manure to relieve Fe, Mn, Zn, Cu and B deficiency in 'Seah' table grapes in Cisakht region of Iran. I International Symposium on Organic Matter Management and Compost Use in Horticulture, 1018: 287-291.

16- Grant, C., Clayton, G., and Johnston, A. 2003. Sulphur fertilizer and tillage effects on canola seed quality in the black soil zone of western Canada. Canadian Journal of Plant Science, 83: 745-758.

17- Hitsuda, K., Yamada, M., and Klepker, D. 2005. Sulfur requirement of eight crops at early stages of growth. Agronomy Journal, 97: 155-159.

18- Hulten, E. 1968. Flora of Alaska and neighboring territories: a manual of the vascular plants. Stanford University Press, USA, pp. 920-921.

19- Janzen, H. and Bettany, J. 1987. The effect of temperature and water potential on sulfur oxidation in soils. Soil Science, 144: 81-89.

20- Kalbas, M., Filsoof, F., and Rezai‐nejad, Y. 1988. Effect of sulfur treatments on yield and uptake of Fe, Zn, and Mn by corn, sorghum, and soybeans. Journal of Plant Nutrition, 11: 1353-1360.

21- Kamtekar, S., Keer, V., and Patil, V. 2014. Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. Journal of Applied Pharmaceutical Science, 4(9): 61-65.

22- Khavazi, K., Nourgholipour, F., and Malakouti, M.J. 2001. Effect of thiobacillus and phosphate solubilizing bacteria on increasing P availability from rock phosphate for corn. In International Meeting on Direct Application of Rock Phosphate and Related Appropriate Technology Latest Development and Practical Experience. Kuala Lumpur, Malaysia. 4(5):330-334

23- Kowalski, R., Sugier, D., Sugier, P., and Kołodziej, B. 2015. Evaluation of the chemical composition of essential oils with respect to the maturity of flower heads of Arnica montana L. and Arnica chamissonis Less. cultivated for industry. Industrial Crops and Products, 76: 857-865.

24- Lawrence, J. and Germida, J. 1988. Relationship between microbial biomass and elemental sulfur oxidation in agricultural soils. Soil Science Society of American Journal, 52: 672-677.

25- Li, X. S., Sato, T., Ooiwa, Y., Kusumi, A., Gu, J. D., and Katayama, Y. 2010. Oxidation of elemental sulfur by Fusarium solani strain THIF01 harboring endobacterium Bradyrhizobium sp. Microbial Ecology, 60: 96-104.

26- Loeppert, R. H. and Suarez, D. L. 1996. Carbonate and gypsum. Publications from USDA-ARS / UNL Faculty, 5: 437-474.

27- Maguire, B. 1943. A monograph of the genus Arnica. Brittonia, 4: 386-510.

28- Malakouti, M.J. 2004. Soil Fertility of Arid and Semi-Arid Regions, Tarbiat Modares University Press, 482 pp.

29- Malakouti, M.J., Baybordi, A., Lotfolahi, M., Shahabi, A., Siavoshi, K., Vakil, R., Ghaderi, J., Shahabifar, J., Majidi, A., and Jafarnejadi, A. 2010. Comparison of complete and sulfur coated urea fertilizers with pre-plant urea in increasing grain yield and nitrogen use efficiency in wheat. Journal of Agricultural Science and Technology, 10: 173-183.

30- Merfort, I. 2003. Arnica: new insights on the molecular mode of action of a traditional medicinal plant. Forschende Komplementarmedizin und klassische Naturheilkunde= Research in complementary and natural classical medicine, 10: 45-48.

31- Modaihsh, A.S., Al-Mustafa W. A., and E., Metwallv A. 1989. Effect of elemental sulfur on chemical changes and nutrient availability in calcareous soils. Plant and Soil, 116: 95-101.

32- Mohammadi Torkashvand, A. 2011. Effect of steel converter slag as iron fertilizer in some calcareous soils. Acta Agriculturae Scandinavica Section B-Soil and Plant Science, 61: 14-22.

33- Radanovic, D., Pljevljakusic, D., Markovic, T., Ristic, M., Dragoja, R., Pljevljakusic, D. and Markovic, T. 2007. Influence of fertilization model and PE mulch on yield and quality of Arnica (A. montana) at dystric cambisol. Zemljište i biljka, 56: 85-95.

34- Salehi, P., Asghari, B., Esmaeili, M. A., Dehghan, H., and Ghazi, I. 2013. α-Glucosidase and α-amylase inhibitory effect and antioxidant activity of ten plant extracts traditionally used in Iran for diabetes. Journal of Medicinal Plants Research, 7: 257-266.

35- Sangtarash, M.H., Qaderi, M.M., Chinnappa, C.C., and Reid, D.M. 2009. Differential sensitivity of canola (Brassica napus) seedlings to ultraviolet-B radiation, water stress and abscisic acid. Environmental and Experimental Botany, 66(2): 212-219.

36- Seyoum, A., Asres, K., and El-Fiky, F.K. 2006. Structure–radical scavenging activity relationships of flavonoids. Phytochemistry, 67(18), : 2058-2070.

37- Singh, R., Bhumbla, D., and Keefer, R. 1995. Recommended soil sulfate-S tests. Recommended soil testing procedures for the Northeastern United States. Northeast Regional Bulletin, 493: 46-51.

38- Small, E. and Catling, P. M. 2000. Les cultures medicinales canadiennes, NRC Research Press.

39- Smallfield, B. and Douglas, M. 2008. Arnica montana a grower’s guide for commercial production in New Zealand. A Report Prepared for New Zealand Arnica Growers’ Group, New Zealand Institute for Crop and Food Research Limited, Christchurch, New Zealand, December, 2.

40- Sorokin, D. Y. 2003. Oxidation of inorganic sulfur compounds by obligately organotrophic bacteria. Microbiology, 72: 641-653.

41- Sparks, D. L., Page, A., Helmke, P., Loeppert, R., Soltanpour, P., Tabatabai, M., Johnston, C., and Sumner, M. 1996. Methods of soil analysis. Part 3-Chemical methods, Soil Science Society of America Inc. 1309 pp.

42- Stursa, J., Kwiatkowski, P., Harcarik, J., Zahradnikova, J., and Krahulec, F. 2009. Cerný a cervený seznam cevnatých rostlin Krkonos/Black and red list of vascular plants of the Krkonose/Karkonosze Mts. (Western Sudetes CZ, PL). Opera Corcontica, 46: 67-104.

43- Sugier, D. 2007. The flowering pattern of Arnica montana L. and A. chamissonis Less. Under field cultivation conditions with successive flower head collection. Acta Agrobotanica, 60 (2): 133-139.

44- Sugier, D. 2013. Yield and chemical composition of mountains arnica (Arnica montana L.) raw material in relation to the method of plantation establishment andthe harvesting time of flower heads. Annales UMCS Agricultura 68 (3), 51-62.

45- Szulc, P., Waligora, H., and Skrzypczak, W. 2008. Better effectiveness of maize fertilization with nitrogen through additional application of magnesium and sulphur. Nauka Przyroda Technologie. Uniwersytet Przyrodniczy w Poznaniu, 2 (3):art. 19.

46- Terry, N. 1976. Effects of sulfur on the photosynthesis of intact leaves and isolated chloroplasts of sugar beets. Plant Physiology, 57: 477-479.

47- Valifard, M., Mohsenzadeh, S., Kholdebarin, B., and Rowshan, V. 2014. Effects of salt stress on volatile compounds, total phenolic content and antioxidant activities of Salvia mirzayanii. South African Journal of Botany, 93:.92-97.

48- Weremczuk-Jeżyna, I., Kisiel, W., and Wysokińska, H. 2006. Thymol derivatives from hairy roots of Arnica montana. Plant Cell Reports, 25: 993-996.

49- Willuhn, G. 1972. Studies on the contents of Arnica species. V. content and variations in content of volatile oils in the various organs of Arnica species. Planta Medica, 21: 221-245.