آنالیز بیان تعدادی از ژن‌های دفاعی در ژنوتیپ حساس و متحمل ذرت در پاسخ به آلودگی ویروس موزاییک کوتولگی ذرت

پژوهش های حفاظت گیاهان ایران

مقاله 1، دوره 32، شماره 4 - شماره پیاپی 42، دی 1397، صفحه 449-461   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v32i4.66848

نویسندگان

فروه السادات مصطفوی¹؛ سید کاظم صباغ^{* 2}؛ احد یامچی³؛ سعید نصرالله نژاد⁴؛ ناصر پنجه که⁵

¹دانشگاه زابل

²یزد

³گرگان

⁴دانشیار دانشکده کشاورزی و منابع طبیعی گرگان

⁵زابل

چکیده

ویروس موزاییک کوتولگی ذرت (Maize dwarf mosaic virus, MDMV) یکی از بیماری­های رایج و محدودکننده رشد در ذرت بشمار می‌رود. در این پژوهش به منظور بررسی نقش مقاومت ژنتیکی در القاء مقاومت دو ژنوتیپ حساس (SC705) و هیبرید متحمل (شماره 8) به MDMV، بیان تعدادی از ژن­های مرتبط با مقاومت شامل Germin like protein (GLP)، Peroxiredoxin (Prx), GF14-6  و S-adenosylmethionine synthase (SAMS) با استفاده از روش سنجش کمی در زمان واقعی مورد بررسی قرار گرفت. مایه­زنی گیاهان با ویروس به روش مکانیکی و در سه تکرار انجام شد. نمونه­برداری در پنج بازه زمانی انجام شد. آنالیز بیان ژن­ها نشان داد که بیان همه ژن­های مورد بررسی در گیاه ذرت متحمل نسبت به حساس افزایش قابل ملاحظه­ای داشته است. اما بیشترین بیان در مورد دو ژن GLP و Peroxiredoxin در بازه زمانی یک ساعت بعد از مایه­زنی مشاهده گردید و برای ژن­های SAMS و GF14-6 بیشترین میزان بیان ژن در بازه زمانی 24 ساعت بعد از مایه‌زنی ثبت شد. کمترین میزان بیان برای ژن­های بررسی شده 72 ساعت بعد از مایه­زنی ویروس مشاهده گردید. با توجه به نتایج به دست آمده و الگوی متفاوت بیان ژن­های مورد بررسی در گیاهان حساس و متحمل می­توان از این ژن­ها به عنوان بیومارکرهای مولکولی در جهت انتخاب ارقام مقاوم به بیماری استفاده نمود.

کلیدواژه‌ها

بیان ژن در زمان واقعی؛ مقاومت ژنتیکی؛ مهندسی ژنتیک؛ ویروس موزاییک کوتولگی ذرت

مراجع

1. Agrios G. 2005. Plant Pathology. 5th Edition. Academic press, USA.

2. Adhikari T.B., Balaji B., Breeden J., and Goodwin S.B. 2007. Resistance of wheat to Mycosphaerella graminicola involves early and late peaks of gene expression. Physiological and Molecular Plant Pathology, 71: 55-68.

3. Beaulieu A., Williams N., and Patience J. 2009. Response to dietary digestible energy concentration in growing pigs fed cereal grain-based diets. Journal of Animal Science, 87: 965-976.

4. Campo S., Peris-Peris C., Montesinos L., Penas G., Messeguer J., and San Segundo B. S. 2012. Expression of the maize ZmGF14-6 gene in rice confers tolerance to drought stress while enhancing susceptibility to pathogen infection. Journal of Experimental Botany, 63(2): 983-99.

5. Chen F., Li Q., Sun L., and He Z. 2006. The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress. DNA Research, 13: 53–63.

6. Cheng J., Chen J., and Chen J. 2002. The complete sequence of a Sugarcane mosaic virus isolate causing Maize dwarf mosaic disease in China. Journal of Science in China, 45: 322-330.

7. Chevalier D., Morris E.R., and Walker J.C. 2009. 14-3-3 and FHA domains mediate phosphoprotein interactions. Annual Review of Plant Biology, 60: 67-91.

8. Converse R.H., and Martin R.R. 1990. ELISA methods for plant viruses. APS Press. pp. 179-196.

9. Denison F. C., Paul A. L., Zupanska A. K., and Ferl R. J. 2011. 14-3-3 proteins in plant physiology. Seminars in Cell and Developmental Biology, 22:720–727.

10. Dietz K. J. 2008. Redox signal integration: from stimulus to networks and genes. Plant Physiology, 133: 459-468.

11. Fomenko D.E., Koc A., Agisheva N., Jacobsen M., Kaya A., Malinouski M., Rutherford J.C., Siu K.C., Jin D.Y., Winqe D.R., and Gladyshev V.N. 2011. Thiol peroxidases mediate specific genome-wide regulation of gene expression in response to hydrogen peroxide. Proceedings of the National Academy of Sciences, 108: 2729-2734.

12. Garcia-Arenal F., Fraile A., and Malpica J.M. 2001. Variability and genetic structure of plant virus populations. Annual Review of Phytopathology, 39: 157-186.

13. Gomez-Gomez L., and Carrasco P. 1998. Differential Expression of the S-Adenosyl-L-Methionine Synthase Genes during Pea Development. Plant Physiology, 117: 397–405.

14. Gucciardo S., Wisniewski J., Brewin N., and Bornemann S. 2006. A germin-like protein with superoxide dismutase activity in pea nodules with high protein sequence identity to a putative rhicadhesin receptor. Journal of Experimental Botany, 1-11.

15. Godfray H.C.J., Beddington J.R., Crute I.R., Haddad L., Lawrence D., Muir J.F., Pretty J., Robinson S., Thomas S.M., and Toulmin C. 2010. Food security: the challenge of feeding 9 billion people. Science, 327: 812-818.

16. Cueto-Ginzo I., Serrano L., Sin E, Rodriguez R., Morales J., Lade S., Medina V., and Achon M. 2016. Exogenous salicylic acid treatment delays initial infection and counteracts alterations induced by Maize dwarf mosaic virus in the maize proteome. Physiological and Molecular Plant Pathology, 96: 47-59.

17. Hao Z., Wang L., and Tao R. 2010. Defense genes and antioxidant enzymes in stage-dependent resistance to rice neck blast. Journal of Plant Pathology, 92(3): 747-752

18. Hao Z., Wang L., Huang F., and Tao R. 2012. Expression of defense genes and antioxidant defense responses in rice resistance to neck blast at the preliminary heading stage and full heading stage. Plant Physiology and Biochemistry. 57: 222-230.

19. Haslekas C., Viken M.K., Grini P. E., Nygaard V., Nordgard S.H., Meza T.J., and Aalen R.B. 2003. Seed 1-Cysteine Peroxiredoxin Antioxidants Are Not Involved in Dormancy, but Contribute to Inhibition of Germination during Stress. Plant Physiology, 133: 1148–1157.

20. Huang Z., Yeakley J.M., Garcia E.W., Holdridge J.D., Fan J.B., and Whitham S.A. 2005. Salicylic acid-dependent expression of host genes in compatible Arabidopsis -virus interactions. Plant Physiology, 137: 1147–1159.

21. Konagaya K.I., Matsushita Y., Kasahara M., and Nyunoya H. 2004. Members of 14-3-3 protein isoforms interacting with the resistance gene product N and the elicitor of Tobacco mosaic virus. Journal of General Plant Pathology, 70: 221–231.

22. Lu G., DeLisle A. J., Vetten N.C., and Ferl R.J. 1992. Brain proteins in plants: An Arabidopsis homolog to neurotransmitter pathway activators is part of a DNA binding complex. Proceedings of the National Academy of Sciences, 89: 11490–11494.

23. Ludmerszki E., Almasi A., Racz I., Szigeti Z., Solti A., Olah C., and Rudnoy S. 2015. S-methylmethionine contributes to enhanced defense against Maize dwarf mosaic virus infection in maize. Brazilian Journal of Botany, 38(4):771–782.

24. Manosalva P.M., Davidson R.M., Xiaoyuan Zhu B.L., Hulbert S.H., Leung H., and Leach J. N. 2009. A Germin-Like Protein Gene Family Functions as a Complex Quantitative Trait Locus Conferring Broad-Spectrum Disease Resistance in Rice. Plant Physiology, 149: 286- 296.

25. Oh C.S., Pedley K.F., and Martin G.B. 2010. Tomato 14-3-3 protein 7 positively regulates immunity-associated programmed cell death by enhancing protein abundance and signaling ability of MAPKKK α. The Plant Cell, 22:260-272.

26. Park Ch.J., An J., Shin Y., Kim K., Lee L., and Paek K. 2004. Molecular characterization of pepper germin-like protein as the novel PR-16 family of pathogenesis-related proteins isolated during the resistance response to viral and bacterial infection. Planta, 219: 797–806.

27. Patnaik D., and Khurana P. 2001. Germin and Germin like protein: an overview. Indian Journal of Experimental Biology, 39:191-200.

28. Pfaffl M.W. 2001. A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Research, 29:2002–2007.

29. Ravanel S., Gakiere B., Job D., and Douce R. 1998. The specific features of methionine biosynthesis and metabolism in plants. Proceedings of the National Academy of Sciences, 95: 7805–7812

30. Rose R., Erdmann S., Bovens S., Wolf A., Rose M., Hennig S., Waldmann H., and Ottmann C. 2010. and Identification and structure of small molecule stabilizers of 14-3-3 protein interaction. Angewandte Chemie International Edition, 49: 4129-4132.

31. Shukla D.D., Jilka J., Tosic M., Ford R.E., Toler R.W., and Langham M.989. Taxonomy of potyviruses infecting maize, sorghum and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology, 79: 223-229.

32. Takusagawa F., Kamitori Sh., Misaki Sh., and Markham G.D. 1996. Crystal Structure of S Adenosylmethionine Synthetase. The Journal of Biological Chemistry, 271(1): 136–140.

33. Tobias I., Bakardjieva N., and Palkovics L. 2007. Comparison of Hungarian and Bulgarian isolates of Maize dwarf mosaic virus. Journal of Cereal Research Communications, 35(4): 1643-1651.

34. Tripathi B.N., Bhatt I., and Dietz K.J. 2009. Peroxiredoxins: A less studied component of hydrogen peroxide detoxification in photosynthetic organisms. Protoplasma, 235: 3-15.

35. Trzmiel K., and Jezewska M. 2008. Identification of Maize dwarf mosaic virus in Maize in Poland. Plant Disease, 92(6): 981-992. Abstract.

36. Uzarowska A., Dionisio G., Sarholz B., Piepho H.P., Xu M., Ingvardsen C.R., Wenzel G., and Lubberstedt T. 2009. Validation of candidate genes putatively associated with resistance to SCMV and MDMV in maize (Zea mays L.) by expression profiling. BMC Plant Biology, 9: 1-15.

37. Wei T., Huang T.S., McNeil J.F., Laliberte J.F., Hong J., Nelson R.S., and Wang A. 2010. of the Sequential recruitment endoplasmic reticulum and chloroplasts for plant potyvirus replication. Journal of Virology, 84: 799–809.

38. Williams M.M., and Pataky J.K. 2012. Interactions between maize dwarf mosaic and weed interference on sweet corn. Field Crop Research, 128: 48–54.

39. Wu L., Wang S., Chen X., Wang X., and Wu L. 2013. Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus. PLoS ONE, 8(7): 1-17.

40. Zimmermann G., Baumlein H., Mock H., Himmelbach A., and Schweizer P. 2006. The multigene family encoding germin-like proteins of barley: regulation and function in basal host resistance. Plant Physiology, 142: 181–19.