Effect of Pseudomonas and Bacillus bacteria on Yield and Nutrient Uptake in Comparison with Chemical and Organic Fertilizers in Wheat

آب و خاک

Article 7, Volume 28, Issue 5, November 2015, Pages 976-986   

Document Type: Research Article

DOI: 10.22067/jsw.v0i0.24371

Authors

A. Fallah Nosrat Abad^{* 1}; Sh. shariati²

¹Soil and Water Research Institute

²Young Researchers and Elite Club, Rasht Branch, Islamic Azad University

Abstract

The high cost of fertilizers in farming systems, soil pollution and degradation of soil are factors that caused to full use of available renewable nutrient sources of plant (organic and biological) with optimal application of fertilizers in order to maintain fertility, structure, biological activity, exchange capacity and water-holding capacity of the water in soil. Therefore, in recent years, according to investigators biofertilizers and organic farming as an alternative to chemical fertilizers has been drawn. Through this study, we examined the effects of triple superphosphate, organic matters and phosphate solubilizing microorganisms on quantitative and qualitative yield of wheat and nutrient uptake. The experiment was carried out in the factorial based on randomized complete block design. The factors were: 1-phosphate solubilizing bacteria in three levels including control, Pseudomonas Putida and Bacillus Coagulans bacteria, 2- triple superphosphate in five levels of 0, 25%, 50%, 75% and 100% and 3-organic matter in 2 levels of 0 and 15 ton/ha in the soil with high phosphorous accessibility (13 mg/kg soil) but lower than sufficient limit for plant 15 mg/kg soil). The results showed that the highest amount of yield has been recorded in Pseudomonas Putida bacteria treatment with organic matter and 25% phosphate fertilizer. As a result, at the conditions of this experiment phosphate solubilizing bacteria and organic matter significantly had higher yield than control and their combination with phosphate fertilizer had significant effect on reducing phosphate fertilizer use.

Keywords

Bacillus; Biofertilizer; Organic matter; Pesuodomonas; wheat

References

رحمانی ا.، و فلاح نصرت آبادی ع. 1380. تولید و ترویج کودهای بیولوژیک محرک رشد گیاه. مجله علوم آب و خاک. جلد 12. صص 105-97.

2- راثی پور ل.، و اصغرزاده ن ع. 1386. اثر متقابل باکتری های حل کننده فسفات و (Bradyrhizobium japonicum) بر شاخص های رشد، غده بندی عناصر غذایی در سویا. مجله علوم و فنون کشاورزی. جلد 11. شماره 40. صص 63-53.

3- فلاح نصرت آبادی ع. 1382. بررسی پراکنش میکروارگانیسم های حل کننده فسفات در خاک های استان گیلان و اثر بخشی آنها در عملکرد گندم و برنج. پایان نامه دکتری، دانشگاه تربیت مدرس. ص 124.

4- لطف الهی م.، ملکوتی م.ج.، خاوازی ک.، و بشارتی ح. 1383. ارزیابی روشهای مصرف مستقیم خاک فسفات در افزایش عملکرد ذرت علوفه‌ای در کرج. در ملکوتی. م.ج و بلالی. م. ر. مصرف بهینه کود راهی برای پایداری در تولیدات کشاورزی نشر آموزش کشاورزی کرج ایران.

5- محمد زاده ع.، و میوه چی لنگرودی ح. 1377. روش مصرف توأم کود حیوانی و فسفره در خاک برای کاهش مصرف کودهای فسفره در خاک های استان بوشهر. نشریه علمی پژوهشی مؤسسه تحقیقات خاک و آب. جلد 12. شماره 1. صص27-20.

6- نورقلی پور ف.، ملکوتی م.ج.، و خاوازی ک. 1380. نقش باکتریهای تیوباسیلوس و حل کننده های فسفات برای افزایش قابلیت جذب فسفر. ضرورت تولید صنعتی کودهای بیولوژیک در کشور (مجموعه مقالات). نشر آموزش کشاورزی به سفارش مؤسسه خاک و آب.

7- Alipour Z.T. and Sobhanipour A. 2012. The Effect of Thiobacillus and Pseudomonas fluorescent Inoculation on maize growth and Fe Uptake, Annals of Biological Research, 3 (3):1661-1666.

8- Arunachalam V. and Bandyopadhyay A. 1984. A method to make decision jointly on a number of dependent characters, Indian Journal of Genetics, 44: 419-424.

9- Banik S. and Dey B.K. 1982. Available phosphate content of an alluvial soil as influenced by inoculation of some isolated phosphate-solubilizing micro-organisms, Plant and soil, 69: 353-364.

10- Carter M. R. and Gregorich E. G. 2008. Soil Sampling and Methods of Analysis. 2nd ed. Canadian Society of Soil Science, 1224.

11- Disimin C.D., Sayer J.A. and Gadd G.M. 1998. Solubilization of zinc phosphate by a strain of Pseudomonase fluorescens isolated from a forest soil, Biology and Fertility of Soils, 28: 87-94.

12- Hameedaa B., Harinib G.O., Rupelab P., Wanib S.P. and Reddya G. 2008. Growth promotion of maize by phosphate solubilizing bacteria isolated from composts and macrofauna, Microbiological Research, 163:234-242.

13- Hofte M.K., Seong Y., Jurkevitch E. and Verstraete W. 1991. Pyoverdin production by the plant growth beneficial Pseuodomonas strain 7SNK2: Ecological significance in soil, Plant and Soil, 130: 249-257.

14- Illmer P. and Schinner F. 1992. Solubilization og inorganic phosphate by microorganisms isolated from forest soils, Soil biology and Biochemistry, 24: 389-395.

15- Khan M.S., Zaidi A. and Wani P.A. 2007. Role of phosphate-solubilizing microorganisms in sustainable agriculture-a review. Agronomy for sustainable development, Agronomy for Sustainable Developments, 27:29–43.

16- Lindsay W.L. and Norvell W.A. 1978. Development of a DTPA soil test for zinc, iron, manganese and copper, Soil Science Society of America Journal, 42: 421-428.

17- Mehboob I., Naveed M. and Zahir Z.A. 2009. Rhizobial association with non-legumes: Mechanismsand application, Critical Reviews in Plant Science, 2009: 28:432-456.

18- Narsian V. and Patel H.H. 2006. Biodiversity of phosphate solubilizing microorganisms in various rhizosphere soils of Bhavnagar district, Asian Journal of Microbiolog, Biotechnology and Enviromental Sciences, 8(2): 201-204.

19- Pradhan N. and Sukla L.B. 2005. Solubilization of inorganic Phosphates by Fungi isolated from agriculture soil, Journal of Biotechnology, 5: 850-854.

20- Puente M. and Bashan Y. 2004. Microbial population and activity in the rhizoplan of rock-weathering desert plants, Growth promotion of cactus sedling. Plant Biology, 6: 643-650.

21- Ryan J., Estefan G. and Rashid R. 2001. Soil and Plant Analysis Laboratory Manual. Second Edition. Available from ICARDA, Aleppo, Syria, 172.

22- Ring R. and Warman P.R. 2000. Phosphorus mineralization from three similar municipal solid waste compost-treated soils by two extraction methods. pp. 449-456.In: Warman P. R., Taylor B. (Eds.), Proceedings of the International Composting Symposium. CBA Press Inc. (Pubs.), Halifax/Darthmouth, Nova Scotia, Canada.

23- Saini V.K., Bhandari S.C. and Tarafdar J.C. 2004. Comparison of crop yield, soil microbial C, N and P, N-fixation, nodulation and mycorrhizal infection in inoculated and non-inoculated sorghum and chickpea crops, Field Crops Research, 89 (l): 39-47.

24- Schachtman D.P., Reid J. and Ayling S.M. 1998. Phosphorus uptake by plants: From Soil to Cell, Plant Physiology, 116:447-453.

25- Sharif M., Chaudhrg F. and Lorho A.G. 1974. Suppression of super phosphate-phosphorus fixation by farmyard manure. Part 2. Soil Science and Plant Nutrition, 20(4):395-401.

26- Sparks D.L. 1996. Method of soil Analysis. Part3. Chemical Methods. American Society of Agronomy. 1390.

27- Subba Rao N.S. 1988. Biofertilizers in Agriculture, Oxford and IBH Publishing Co., New Delhi, 208.

28- Tian G. and Kolawole G.O. 2004. Comparison of various plant residues as phosphate rock amendment on Savanna Soils of West Africa, Journal of plant nutrition, 27: (4) 571-583.

29- Vassilev N., Vassileva M. and Nikolaeva I. 2006. Simultaneous P-solubilizing and biocontrol activity of microorganisms: Potential and future trends, Applied Microbiol Biotechnology, 71: 137-144.

30- Yahya A.J. and Al-Alzawi S.K. 1989. Occurrence solubilizing bacteria in some Iraqi soils. Plant and Soil, 117: 135-141.

31- Yazdani M., Bahmanyar M.A., Pirdashti H. and Esmaili M.A. 2009. Effect of phosphate solublization bacteria microorganisms (PSM) and plant growth promoting rhizobacteria (PGPR) on yield and yield components of corn (Zea mays L.).World academy of science, Engineering and technology, 49:90-92.