Effect of Deficit Irrigation Regimes and Foliar Application of Jasmonic Acid and Ascorbic Acid on Some Secondary Metabolites, Oil Yield and Grain Yield of Safflower Genotypes

پژوهشهای زراعی ایران

Article 7, Volume 17, Issue 4 - Serial Number 56, January 2020, Pages 591-603   

Document Type: Research Article

DOI: 10.22067/gsc.v17i4.77709

Authors

F Mohtashamii; M. R Tadayon^* ; P Roshandel

Shahrekord University

Abstract

Introduction
Safflower is an annual plant native to the Mediterranean countries and cultivated in Europe and U.S. Safflower petals are very important as a source of medicinal preparations, natural food color and dyes for coloring fabrics. Water deficit stress severely limits crop growth especially in arid and semiarid regions of the world as it affects all stages of plant growth and development. Exposure to environmental stresses such as drought stress, heat stress, cold stress, salt stress and plant diseases often leads to the production of reactive oxygen species and other toxic compounds that diminish a plant’s performance. Reactive oxygen species (ROS) are highly toxic to plant cells and in an absence of any protective mechanism they can react with proteins, lipids and DNA and this can inactivate an antioxidant defense system. Plants have an elaborate system of enzymatic and non-enzymatic scavenging pathways or detoxification systems that working together function as an extremely efficient system to counter the deleterious effects of ROS. Higher plants have active oxygen-scavenging systems consisting of several antioxidant enzymes, and some low molecules of non-enzyme antioxidants, such as phenolic compounds, flavonoids, anthocyanins and ascorbic acid. Water deficit is considered to be a major abiotic factor affecting many aspects of plant physiology and biochemistry causing a significant reduction in agricultural production and changes its behavior regarding the biosynthesis of bioactive compounds such as phenolic compounds, flavonoids and anthocyanins in safflower. Ascorbic Acid is a major metabolite in plants. It is an antioxidant, in association with other components of the antioxidant system, protects plants against oxidative damage resulting from aerobic metabolism. Jasmonic acid (JA) is a plant-signaling molecule that shows a wide range of plant responses, with effects at the morphological to molecular levels. Many morphological, physiological, and biochemical processes occurring within the plants can be regulated by jasmonic acid. Previous studies have documented that foliar application of JA and ascorbic acid could modulate plant physiological responses towards abiotic stress tolerance. Considering the importance of safflower plant in terms of quantity and quality of oil, and its various applications in the oil, dyeing and pharmaceutical industries in the semi-arid regions of the world, it seems that increasing yield per unit area in low-irrigated conditions is a good way to increase the production of this plant and improve the economic conditions of farmers. Compared to breeding methods that are often long-term and costly, some agricultural management practices, such as the use of chemicals like ascorbic acid, jasmine acid and other compounds, are easier, cheaper and faster. Therefore, the aim of this study was to determine the effects of jasmonic acid and ascorbic acid on grain yield and Changes in antioxidant compounds of safflower genotypes under drought stress conditions.
Materials and Methods
This experiment was carried out as a split-split plot in a completely randomized block design with three replications at Shahrekord University Agricultural Research Station during spring planting season 2017. The main plots consisted of three levels of irrigation of 100%, 75% and 50% of the plant's water requirement of safflower and sub-plots including safflower genotypes including Sinai, Isfahan local and Faramanand sub-sub sub-plots including foliar application with three levels including ( control, foliar application of jasmonic acid with 0.5mM concentration and foliar application of ascorbic acid with 20 mM concentration. In this study, the amount of total flavonoid, total phenol, antioxidant activity, ammonialase enzyme activity, grain yield and oil yield were measured.
Results and Discussion
The results showed that simple effects of low irrigation treatment, genotype and foliar application were significantly in all traits studied in this study. The highest grain yield (1609 kg.ha-1) and oil yield (430 kg.ha-1) obtained in 100% of the plant's water requirement and the least of grain yield (1382 kg.ha-1) and oil yield (335 kg.ha-1) obtained in 50% of the plant's water requirement. The interaction of genotype × deficit irrigation treatments on total phenol content, total flavonoid content, phenylalanine ammonialase enzyme activity and oil yield as well as interaction of low irrigation treatment × foliar application on the amount of antioxidant activity, total phenol, total flavonoid content were significant. The results show that the highest total phenol content (35.4 mg GA. g-1 DW), total flavonoid content (12.1 mg quercetin .g-1 DW), phenylalanine ammonialase enzyme activity (28.6 µmol.g -1FW. min) obtained in interaction 50% of the plant's water requirement and sina genotype and the least amount oftotal phenol content (17.6 mg GA. g-1 DW), total flavonoid content (5.2mg quercetin . g-1DW), phenylalanine ammonialase enzyme activity (8.4 µmol.g -1FW. min ) obtained in interaction 100% of the plant's water requirement and Isfahan genotype. Foliar application with jasmonic acid and ascorbic acid increased antioxidant activity, total phenol, total flavonoid, phenylalanine ammonialase enzyme activity, oil yield and grain yield. So that, the highest amount of antioxidant activity (85.9 % inhibition of linoleic acid), total phenol (31.7 mg GA. g-1 DW) and total flavonoid (11.7 mg quercetin . g-1 DW) related to 50% of the plant's water requirement and foliar application with ascorbic acid and the least amount of antioxidant activity (54.7 antioxidant activity), total phenol (17.6 mg GA. g-1DW) and total flavonoid (5.2 mg quercetin . g-1 DW) related to 100% of the plant's water requirement and without foliar application.
Conclusions
Results indicated that deficit irrigation stress could increase antioxidant activity, Phenylalanine ammonia lyase (PAL) activity, Total phenol, total flavonoid and anthocyanin content in safflower genotype but, grain yield decreased. The increase in tolerance to drought in sina genotype is associated with antioxidant enzyme and non-enzyme activity. Also foliar application of jasmonic acid and ascorbic acid increase antioxidant activity, Phenylalanine ammonia lyase (PAL) activity, Total phenol, Total flavonoid, anthocyanin content and grain yield. According to these results, it may be suggested that increased activity of antioxidant enzymes and non-enzymes can reduce the harmful effects of reactive oxygen species and improve plant drought tolerance, therefore antioxidant enzymes and non-enzymes can be taken as indices of drought tolerance in plants.

Keywords

Anthocyanin; Flavonoid; Phenol; Phenylalanine ammonialase

References

1. Amini, H., Arzani, A., and Bahrami, F. 2013. Seed yield and some physiological traits of safflower as affected by water deficit stress. International Journal of Plant Production 7 (3): 598-614.

2. Arab, S., Baradaran Firouzabadi, M., Asghari, H., Gholami, A., and Rahimi, M. 2016. The effect of ascorbic acid and sodium nitroprusside foliar application on seed yield, oil and some agronomical traits of safflower (Carthamus tinctorius L.) under water deficit stress. Environmental Stresses Crop Sciences 9 (1): 15-27. (in Persian with English abstract).

3. Asqarpanh, J., and Kazemivash, N. 2013. pharmacology and medicinal properties of (Carthamus tinctorius L.). Chines Journal of integrative Medicine 19 (2): 53-59.

4. Behdani, M., and Jami Al-Mahdi, M. 2010. Response of Spring Safflower Cultivars to Irrigation Intervals in Birjand Condition. Iranian Journal Field Crops Research 8 (2): 315-335. (in Persian with English abstract).

5. Bagal, U. R., Leebens mack, J. H., Walter Lorenz, W., and Dean, J. F. D. 2012. The phenylalanine ammonia lyase (PAL) gene family shows a gymnosperm specific line age. BioMed Central Genomics 13 )3:( 1471-2164.

6. Bari, R., and Jones, J. D. 2009. Role of plant hormones in plant defense responses. Plant Molecular Biology 69: 473-488.

7. Campbell, M. M., and Ellis, B. E. 1992. Fungal elicitor-mediated responses in pine cell cultures: III. Purification and characterization of phenylalanine ammonia-lyase. Plant Physiology 98: 62-70.

8. Chen, Y., Xie, M. Y., and Gong, X. F. 2007. Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum. Journal of Food Engineering 81: 162-170.

9. Creelman, R. A., and Mullet, J. E. 1995. Jasmonic acid distribution and action in plants: regulation during development and response to biotic and abiotic stress. Proceedings of the National Academy of Sciences USA 92: 4114-4119.

10. Daneshmand, F. 2014. The effect of ascorbic acid on reduction of oxidative stress caused by salinity in potato. Journal of Plant Researches 27 (3): 417-426.

11. Dixon, R. A., Ferreira, D., and Genistein, S. 2002. The phenylpropanoid pathway and plant defense - a genomics perspective. Molecular Plant Pathology 3: 371-390.

12. Dolatabadian, A., Modares Sanavy, A. M., and Asilan, K. 2009. Effect of ascorbic acid foliar application on yield, yield component and several morphological traits of grain corn under water deficit stress conditions. Journal of Notulae Scientia Biologicae 2 (3): 45-50.

13. Ebrahimzadeh, M., Hosseinimehr, S., Hamidinia, A., and Jafari, M. 2008. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharm ecology online 1: 7-14.

14. Edwards, K., Cramer, C. L., Bolwell, G. P., Dixon, R. A., Schuch, W., and Lamb, C. J. 1985. Rapid transient induction of phenylalanine ammonia-lyase mRNA in elicitor-treated bean cells. Proceedings of the National Academy of Sciences of the United States of America 82: 6731-6735.

15. Fanaie, H. M., Keikha, H., and Piri, E. 2015. Effect of seed priming on grain and oil yield of Safflower under irrigation deficit conditions. Iranian Journal of Seed Science and Research 2 (2): 49-59. (in Persian with English abstract).

16. Farshi, A. A. 2003. Irrigation Water Management at the Field. Iranian National Committee on Irrigation and Drainage. Ahvaz. (Persian Book).

17. Ferasat, M., Sajadi, M. A., Mirzakhani, M. 2008. Response of agriculture traits to drought stress condition in four safflower genotypes. New Finding in Agriculture 3: 67-81.

18. Ghassemi-Golezani, K., and Hosseinzadeh-Mahootchi, A. 2015. Improving physiological performance of safflower under salt stress by application of salicylic acid and jasmonic acid. Walia Journal 31: 104-109.

19. He, Q., and Luo, Y. 2007. Enzymatic browning and its control in fresh-cut produce. Stewart Postharvest Review 3: 123-132.

20. Hernandez, I., Alegre, L., and Munne-Bosch, S. 2004. Drought-induced changes in flavonoids and other low molecular weight antioxidants in Cistus chisii grown under Mediterranean field conditions. Tree Physiology 24: 1303-1311.

21. Kartal, N., Sokmen, M., Tepe, B., Daferera, D., Polissiou, M., and Sokmen, A. 2007. Investigation of the antioxidant properties Ferula orientalis L. using a suitable extraction procedure. Food Chemistry 100 (2): 584-589.

22. Khalili, M., Naghavi, M. R., and Pour-Aboughadareh, A. 2016. Evaluation of Grain Yield and Some of Agro-Morphological Characters in Spring Safflowers Genotypes under Irrigated and Rainfed Conditions. Journal of Crop Breeding 7 (16): 139-138. (in Persian with English abstract).

23. Koukal, J., and Conn, E. E. 1961. The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminize of Hordeum vulgar. Journal of Biological Chemistry 236: 2692-2698.

24. Mahabub Alam, M. D., Kamrun, N., Hasanuzzaman, M., and Masayuki, F. 2014. Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species. Journal of Plant Biotechnology Reports 8: 279-293.

25. Mahmood, M., Bidabadi, S. S., Ghobadi, C., and Gray, D. J. 2012. Effect of methyl jasmonate treatments on alleviation of polyethylene glycol-mediated water stress in banana (Musa acuminata) shoot tip cultures. Plant Growth Regulation 68: 161-169.

26. Marinova, D., Ribarov, F., and Atanassova, M. 2005. Total phenolic and total flavonoids in Bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy 40 (3): 255-260.

27. Miller, G., Suzuki, N., and Ciftci‐Yilmaz, S. 2010. Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell and Environment 33: 453-467.

28. Mittler, R. 2004. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7: 405-410.

29. Moradi Tochali, M., Seiphzade, S., Zakerin, H. M., and Valadabadi, A. R. 2017. Investigation the effect of methanol and ascorbic acid foliar application on growth and yield of peanut (Arachis hypogaea L.). Crop Physiology Journal 9 (36): 65-82. (in Persian with English abstract).

30. Ozeki, Y., and Komamine, A. 1985. Changes in activities of enzymes involved in general phenylpropanoid metabolism during the induction and reduction of anthocyanin synthesis in a carrot suspension culture as regulated by 2,4-D. Plant Cell Physiology 26: 903-911.

31. Ramishkrishna, A., Ravishankar, G. A. 2011. Influence of abiotic stress signals on secondary metabolitis in plant. Journal of plant Signaling and Behavior 6: 1720-1731.

32. Ritter, H., and Schulz, G. E. 2004. Structural basis for the entrance into the phenylpropanoid nucleic metabolism catalyzed by phenylalanine ammonia-lyase. Plant Cell 16 (12): 3426-3436.

33. Sajadi, N. M., Ferasat, M., and Mirzakhani, M. 2012. Impact of water deficit stress on biochemical characteristics of safflower cultivars. Physiology and Molecular Biology of Plants 18 (4): 323-329.

34. Salama Zeinab, A., El-Fouly, M. M., and Gaafor, A. A. 2013. Mitigation of the adverse effect of salinity through stimulation some secondary metabolites and antioxidant enzymes of metabolic extract of maize cultivars by exogenous ascorbic acid. Journal of food, Agriculture of Environment 11: 1328-1335.

35. Schaller, F. 2001. Enzymes of the biosynthesis of octadecanoid-derivedsignaling molecules. Journal Experimental Botany 52: 11-23.

36. Sio- Se Mardeh, A., Ahmadi, A., Poustini, K., and Mohammadi, V. 2006. Evaluation of drought resistance indices under various environmental conditions. Field Crop Research 98: 222-229.

37. Vwioko, E. D., Osawaru, M. E., and Erugun, O. L. 2008. Evaluation of okro (Abelmoschus esculentus L. Moech). Exposed to paint waste contaminated soil for growth, ascorbic acid and metal concentration. African Journal of General Agriculture 4 (1): 39-48.

38. Wang, J. W., Zheng, L. P., Wu, J. Y., and Tan, R. Y. 2006. Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and Taxol production induced by low-energy ultrasound in Taxus yunn an ensiscell suspension cultures. Journal of Nitric Oxide 15: 351-358.

39. Winkle-Shirley, B. 2001. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiology 126: 485-493.

40. Zhao, J., Davis, L. C., Verpoorte, R. 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances 23 (4): 283-333.