Reaction of Sweet Basil (Ocimum bacilicum L.) to Different Fertilizing System in an Agroecosystem

علوم باغبانی

Article 7, Volume 35, Issue 4 - Serial Number 52, January 2022, Pages 535-547   

Document Type: Research Article

DOI: 10.22067/jhs.2021.61907.0

Authors

S. Charbandi¹; F. Zaefarian^{* 1}; V. Akbarpour²; M. Kaveh¹

¹Department of Agronomy, Faculty of Crop Sceiences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

²Department of Horticulture, Faculty of Crop Sceiences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

Abstract

Introduction: The tendency to produce aromatic and medicinal plants and the demand for natural products is increasing especially in the world. Ecological agriculture (Agroecology) of medicinal plants guarantees their quality and reduces the possibility of negative effects on their quality and yield. Evaluation of different plant nutrition systems is one of the most important principles in the production planning of medicinal plants in order to achieve high yield and quality of effective ingredients. Proper fertilizer management is one of the most important factors in agricultural production. Identification of fertilizers which are compatible with nature and are suitable for plant growth could have favorable effects on quantitative and qualitative indices of the plant. Organic fertilizers are genuine and risk-free products that could be suitable for agricultural sustainability. Soil organic matter improves fertility and water retention, provides proper ventilation, long-term storage of plant nutrients and microorganisms, so replacing chemical fertilizers with fertilizers such as vermicompost and biofertilizer can reduce the above-mentioned effects. Biofertilizers are one of the operations that is nowadays in accordance with the principles of sustainable agriculture for soil fertility. Biofertilizers include the compost of one or more types of useful soil organisms or as metabolic by-products of these organisms that are used to provide the plant with the nutrients it needs in an agroecosystem.
Materials and Methods: In order to investigate the effect of biochar, organic and biological fertilizers on the quantity and quality of Ocimum bacilicum L., a factorial experiment was done in a randomized complete block design with three replications. Experimental treatments included two levels of biochar (0 and 20 ton ha^-1) and five levels of fertilizers (vermicompost (10 ton ha^-1), Mycorrhiza, Azotobacter, Pseudomonas and no fertilizer application). Prior to planting sweet basil seed, biofertilizers and vermicompost were mixed with soil to the depth of 15 cm. Mycorrhizal fungi were mixed with seed. Azotobacter and Pseudomonas bacteria were also incubated with seeds (seed inoculated). It should be added that in this study no chemicals were used to control weeds and pests.
Results and Discussion: According to the results, the highest plant height and number of main branches were obtained in biochar application with vermicompost (49.37 cm and 7, respectively), which was significantly better than other treatments. The interaction effect of biochar and fertilizer had a significant effect on fresh and dry weight of leaf and stem. Mean comparison of treatments showed that the highest leaf and stem fresh weight were obtained in biochar + Pseudomonas application (792 and 876 g m^-2, respectively), which had no significant difference with biochar + vermicompost; and also, the maximum leaf dry weight and stem dry weight were obtained in biochar with Pseudomonas application (166 and 175 g m^-2, respectively). The interaction between biochar and fertilizer on phenol, flavonoids and antioxidant activity of basil showed a significant differences between treatments.
Conclusion: In general, the results showed that the use of biochar and biological and organic fertilizers could be effective in both the quality and quantity of sweet bail, where, the use of the bacterium Azobacter and Pseudomonas with biochar had the greatest effect on the quality and quantity of sweet bail. Finally, it could be concluded that the combined use of biochar with biological fertilizers had significant impacts on the quantitative and qualitative traits of sweet bail in ecological or low input agricultural.

Keywords

Azotobacter; Biofertilizer; Mycorrhiza; Organic Fertilizer; Pseudomonas

References

1. Al-Farsi M., Alsalvar C., Morris A., Baron M., and Shadih F. 2005. Comparison of antioxidant activity, anthocyanins, carotenoids and phenolics of three native fresh and sun-dried date (Phoenix dactylifera) varieties grown in Oman. Journal of Agricultural and Food Chemistry 53: 7592-7599.
2. Arjmand Alavi M., Hatamzadeh A., and Ehteshami S.M.R. 2014. Effect of bulb inoculation with four species mycorrhizal fungi on quantitative and qualitative yield of two lily species. Seed Science and Research 1(2): 57-65. (In Persian)
3. Bamminger C., Marschner B., and Juschke E. 2014. An incubation study on the stability and biological effects of pyrogenic and hydrothermal biochar in two soils. European Journal of Soil Science 65(1): 72-82.
4. Behnam H., Farrokhian Firouzi A., and MoezziA. 2016. Effect of biochar and compost sugarcane bagasse on some soil mechanical properties. Water and Soil Conservation 23(4): 235-250. (In Persian)
5. Chang C., Yang M., Wen H., and Chern J. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 10: 178-182.
6. Ebrahimzadeh Abdashti R. 2013. Effects of organic and chemical fertilizers on the quantitative and qualitative yield of sour tea (Hibiscus sabdaiffa). M.Sc Thesis, University of Zabol. 110 pages.
7. Emami Bistagani Z., Siadat AA, Bakhshandeh A.M. And Ghasemi Pirblouti A.A. 2016. The effect of chemical fertilizers, organic chitosan on physiological characteristics and phenolic compounds of thyme in the Shahrekord region. Agricultural Research, 7 (1): 26-11.
8. Faraji Mehmani A., Esmaielpour B., Sefidkon F., Abbaszadeh B., Khavazi K., and Ghanbari A.R. Effects of biofertilizers on growth criteria, quantitative and qualitative yield of summer savory (Satureja hortensis L.). Agroecology 6(4): 870-879. (In Persian)
9. Ghasemzadeh A., and Jaafar H.Z.E. 2011. Effect of CO₂ enrichment on synthesis of some primary and secondary metabolites in ginger (Zingiber officinale Roscoe). International Journal of Molecular Sciences 12(2): 1101-1114.
10. Gholami Sharafkhane E., Jahan M., Banayan Avval M., Koocheki A., and Rezvani Moghaddam P. 2015. The effect of organic, biological and chemical fertilizers on yield, essential oil percentage and some agroecological characteristics of summer savory (Satureja hortensis ) under Mashhad conditions. Journal of Agroecology 7: 179-189. (In Persian)
11. Ghorbanali M., Saadatmand L., and Nikan M. 2011. The effects of natural habitat on polyphenol flavonoids, anthocyanin and its related antioxidant activity in Silverberry. The First National Conference on Modern Topics in Agriculture, Saveh, Islamic Azad University,8 November, (In Persian)
12. Han H.S., and Lee K.D. 2006. Effect of inoculate on with phosphate and potassium co-in solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant, Soil and Environment 52: 130-136.
13. Hatami S.F., Esmaielpour B., Hadian J., Khavazi K., Soltani Toolarood A.A., and Abbaszadeh Dahaji P. 2014. Effects of biofertilizers on morphological traits of tarragon. Soil Biology 2(1): 55-63. (In Persian)
14. Hejazizadeh A., Gholam Alizadeh Ahangar A., and Ghorbani M. 2016. Effect of biochar on lead and cadmium uptake from applied paper factory sewage sludge by sunflower (Heliantus annus). Water and Soil Science 26(2): 259-271. (In Persian)
15. Islam F., Yasmeen T., Ali Q., Ali S., Arif M.S., Hussain S., and Rizvi H. 2014. Influence of Pseudomonas aeruginosa as PGPR on oxidative stress tolerance in wheat under Zn stress. Ecotoxicology and Environmetal Safety 104: 285-293.
16. Kiani Z., Esmaeilpour B., Hadian J., Soltani Toolarood A.A., and Fatah Aloumi S. 2015. Effect of organic fertilizers on growth properties nutrient absorption and essential oil yield of medicinal plant of spearmint. Plant Production 21(4): 63-80.
17. Mahmoudzadeh M., Rasouli Sadaghiani M.H., and Asgari Lajayer H. 2016. Effect of plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi on growth characteristics and concentration of macronutrients in peppermint (Mentha pipperita) under greenhouse conditions. Journal of Science and Technology of Greenhouse Culture Soilless Culture Research Center 6(4): 155-168. (In Persian)
18. Makkizadeh Tafti M., Chaichi M., Nasrollahzadeh S., and Khavazi K. 2012. Effect of different types of nitrogen fertilizers on quantitative and qualitative characteristics of Satureja hortensis Iranian Journal of Medicinal and Aromatic Plants 28: 330-341.
19. Maleki V., Salimi K.H., and Ardakani M.R. 2016. Effect of biofertilizers on vegetative growth and essential oil of sweet basil. Agroecology 11(4): 49-56. (In Persian)
20. Masulili A., and Utomo W.H. 2010. Rice husk biochar for rice based cropping system in acid soil 1. The characteristics of rice husk biochar and its influence on the properties of acid sulfate soils and rice growth in west Kalimantan, Indonesia. Journal of Agricultural Science pp: 39-47.
21. Mckey D. 1979. The Distribution of Secondary Compounds within Plants. In: Rosenthal G.A., Janzen D.H., editors, Herbivores: their interaction with secondary plant metabolites. New York: Academic Press.
22. Miliauskas G.P., Venskutonis R., and Vanbeek T.A. 2004. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry 85: 231-237.
23. Montanari M., Innocenti E.D., Maggini R., Pacifici S., Pardossi A. and Guidi L. 2008. Effect of nitrate fertilization and saline stress on the contents of active constituents of Echinacea angustifolia Food Chemistry 107: 1461-1466.
24. Mousavi F., Solymani A., Rezaiinezhad A.H., and Kheyri A.A. 2014. The effect of vermicompost on some quantitative and qualitative properties of peppermint. The First National Conference on New Ideas in Sustainable Agriculture, March 5, Boroujerd, Islamic Azad University. (In Persian)
25. Mukherjee A., and Zimmerman A.R. 2013. Organic carbon and nutrient release from a range of laboratory-produced biochars and biochar-soil mixtures. Geoderma 193(1): 122-130.
26. Mullera V., Lankesa C., Zimmermannb B.F., Nogaa G., and Hunschea M. 2013. Centelloside accumulation in leaves of Centella asiatica is determined by resource partitioning between primary and secondary metabolism while influenced by supply levels of either nitrogen, phosphorus or potassium. Journal of Plant Physiology 170: 1165-1175.
27. Naguib A.M., El-Baz F.K., Salama Z.A., Hanaa A.B., Hanaa F.A., and Gaafar A.A. 2012. Enhancement of phenolics, flavonoids and glucosinolates of broccoli (Brassica olaracea Italica) as antioxidants in response to organic and bio-organic fertilizers. Journal of the Saudi Society of Agricultural Sciences 11(1): 135-142.
28. Nouri Hosseini M., Khorasani R., Astaraei AR., Rezvani Moghaddam P., and Zabihi H.R. 2016. The effect of different fertilizer sources and humic acid on morphological characteristics, yield and antioxidant content of black cumin. Applied Agricultural Research 29(4): 104-87.
29. Nur M.S.M., Islami T., Handayanto E., Nugroho W.H., and Utomo W.H. 2014. The use of biochar fortified compost on calcareous soil of east Nusa Tenggara, Indonesia: 2. Effect on the yield of maize (Zea mays) and phosphate absorption. American-Eurasian Journal of Sustainable Agriculture 8(5): 105-111.
30. Omidbaigi R. 1997. Approaches to Production and Processing of Medicinal Plants. Tarahane'e Nashr publication. Vol 2. Tehran-Iran. 424 pp. (In Persian)
31. Pirasteh Anosheh H., Emam Y., and Jamali Ramin F. 2010. Comparative effect of biofertilizers with chemical fertilizers on sunflower (Helianthus annuus) growth, yield and oil percentage in different drought stress levels. Agroecology 2(3): 492-501. (In Persian)
32. Ramezanpour M., Popov Y., Khavazi K., and Asadi Rahmani H. 2010. Genetic diversity and efficiency of indole acetic acid production by the isolates of fluorescent pseudomonads from rhizosphere of rice (Oryza sativa). American-Eurasian Journal of Agriculture and Environmental Science 7(1): 103-109.
33. Rashid M., Khalil S., Ayub N., Alam S., and Latif F. 2004. Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Pakistan Journal of Biological Science 7: 187-196.
34. Rezaee Moadab., S.M., Nabavi Kalat R., and Sadrabadi Haghaghi. Effects of biological fertilizer and vermicompost on vegetative yield and essential oil of basil (Ocimum basilicum L.) under Mashhad climatic conditions. Agroecology 5(4): 350-362.
35. Rezaeeyan A. 2014. Effect of biochar and arbuscular mycorrhiza on cadmium absorption, transfer and accumulation in peppermint.Sc Thesis, Shahroud University, Iran. (In Persian)
36. Sajadinik R., and Yadavi A.R. 2013. Effect of nitrogen fertilizer, vermicompost and nitroxin on growth indices, phenological stages and grain yield of sesame. Crop Production 6(2): 73-100. (In Persian)
37. Schmidt M.W.I., and Noack A.G. 2000. Black carbon in soils and sediments: analysis, distribution, implications and current challenges. Global Biogeochemical Cycle 14: 777-793.
38. Shams S., and Bagherzadeh Chharjoei A. 2013. The effect of different amounts of two types of biological fertilizer, vermicompost and cattle manure on morphological characters and active ingredients content of basil. The Third National Conference on Agricultural Science and Food Industry. Fasa University 1-7. (In Persian)
39. Sharma A.K. 2002. Bio-fertilizers for Sustainable Agriculture. 1st edition. Jodhpur: agrobios, Indian, 456p.
40. Singh B.P., Hatton B.J., Singh B., Cowie A., and Kathuria A. 2010. Influence of biochars on nitrous oxide emission and nitrogen leaching from two contrasting soils. Journal of Environmental Quality 39: 1224-1235.
41. Stajkovic O., Delic D., Josic D., Kuzmanovic D., Rasulic N., and Knezevic-Vukcevic J. 2011. Improvement of common bean growth-promoting bacteria. Romanian Biotechnological Letters 16(1): 5919-5926.
42. Tahami–Zarandi M.K. 2010. Assessment of organic, biologic and fertilizer on yield, yield components and essence of basil .MSc. dissertation, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran. (In Persian)
43. Tahat M.M., and Sijan K. 2012. Mycorrhizal fungi and abiotic environmental conditions relationship. Research Journal of Environmental Science 6: 125-133.
44. Vojodi Mehrabani L., Valizadeh Kamran R., and Hassanpour Aghdam M.B. 2017. The effects of relative substitution of organic fertilizers on elementes content, some physiological traits and yield of Lepidium sativum Agriculture Sciencs and Sustainable Production 27(3): 63-72. (In Persian)
45. Watzinger A., Feichtmair S., Kitzler B., Zehetner F., Kloss S., Wimmer B., Boltenstern S.Z., and Soja G. 2014. Soil microbial communities responded to biochar application in temperate soils and slowly metabolized ¹³C-labelled biochar as revealed by ¹³C PLFA analysis: results from a short term incubation and pot experiment. European Journal of Soil Science 65(1): 40-51.
46. Zheng Y., Dixon M., and Saxena P.K. 2006. Growing environment and nutrient availability affect the content of some phenolic compounds in Echinacea purpurea and Echinacea angustifolia. Planta Medica 72: 1407-1414.