بررسی مدت زمان استفاده از اسفنج‌های پروژسترون‌دار همراه با تزریق هورمون‌های استرادیول و eCG بر عملکرد تولیدمثلی در گوسفند

پژوهشهای علوم دامی ایران

مقاله 10، دوره 15، شماره 1 - شماره پیاپی 53، فروردین 1402، صفحه 123-135   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.22067/ijasr.2022.75088.1066

نویسندگان

محمد رضا بحرینی بهزادی^* ؛ محمد کشاورزپور؛ فرهاد صمدیان

گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج، یاسوج، ایران.

چکیده

هدف از انجام این پژوهش، بررسی عملکرد تولیدمثلی میش‌های بومی شهرستان خانمیرزا در دو فصل تولیدمثلی و غیر تولیدمثلی، با برنامه‏های کوتاه‎مدت و بلندمدت همزمان‎سازی فحلی به همراه تزریق استرادیول و eCG بود. آزمایش در دو دوره و با دو گروه مجزای 400 رأسی از میش‏ها (دو تا پنج سال و میانگین وزنی 5/2±45 کیلوگرم) در قالب چهار گروه آزمایشی 100 رأسی انجام شد. در گروه اول یا شاهد هیچ‌گونه برنامه همزمان‏سازی انجام نشد. گروه‏های آزمایشی دوم و سوم، تیمارهای کوتاه‏مدت استفاده از اسفنج پروژسترونی به‌مدت 12 روز بودند. در تیمارهای دوم و سوم در روز اسفنج‏برداری به‌ترتیب یک میلی‏گرم استرادیول بنزوات و 400 واحد بین‌المللی eCG به‌طور عضلانی تزریق شد. در تیمار چهارم یا تیمار بلندمدت-eCG، میش‎ها به‌مدت 14 روز تیمار پروژسترونی را دریافت کرده و در روز اسفنج‏برداری 400 واحد بین‌المللی eCG به‌طور عضلانی به آن‌ها تزریق شد. نتایج نشان داد که کاهش طول مدت تیمار با اسفنج پروژسترونی، در هر دو دوره آزمایش، منجر به کاهش معنی‏دار نرخ پاسخ فحلی گردید، ولی بر سایر فراسنجه‏های تولیدمثلی تأثیر معنی‏داری نداشت. صرف نظر از فصل، تزریق استرادیول به‌جای eCG در روز اسفنج‏برداری، ضمن افزایش نرخ پاسخ فحلی میش‏ها، منجر به افزایش شدید نرخ بازگشت به فحلی و کاهش معنی‏دار درصد میش‎های آبستن، میش‎های زایمان کرده و نرخ بره‎زایی میش‎ها گردید. بنابراین، در طول هر دو فصل تولیدمثلی و غیر تولیدمثلی، تیمار کوتاه‏مدت پروژسترونی (12 روز) و همچنین تزریق استرادیول بنزوات به‌جای eCG برای همزمان‏سازی فحلی میش‎ها قابل توصیه نمی‏باشد.

کلیدواژه‌ها

اسفنج پروژسترون‌دار؛ عملکرد تولیدمثلی؛ گوسفند؛ همزمان‌سازی فحلی

مراجع

1. Abecia, J. A., Forcada, F., & González-Bulnes, A. (2012). Hormonal control of reproduction in small ruminants. Animal Reproduction Science, 130(3-4), 173-179. doi:10.1016/j.anireprosci.2012.01.011.
2. Ahmed Amer, H., & Maher Hazzaa, A. (2009). The effect of different progesterone protocols on the reproductive efficiency of ewes during the non-breeding season. Veterinarski Arhiv, 79(1), 19-30.
3. Almadaly, E., Ashour, M., El-Kon, I., Heleil, B., & Fattouh, E. S. (2016). Efficacy of various synchronization protocols on the estrus behavior, lambing rate and prolificacy in Rahman Egyptian ewes during the non-breeding season. Asian Journal of Animal and Veterinary Advances, 11(1), 34-43.
4. Ataman, M. B., Akoz, M., & Akman, O. (2006). Induction of synchronized oestrus in Akkaraman cross-bred ewes during breeding and anestrus seasons: the use of short-term and long-term progesterone treatments. Revue de Médecine Vétérinaire, 157(5), 257-260.
5. Barrett, D. M. W., Bartlewski, P. M., Duggavathi, R., Davies, K. L., Huchkowsky, S. L., Epp, T., & Rawlings, N. C. (2008). Synchronization of follicular wave emergence in the seasonally anestrous ewe: The effects of estradiol with or without medroxyprogesterone acetate. Theriogenology, 69(7), 827-836. https://doi.org/10.1016/j.theriogenology.2007.12.010.
6. Beck, N. F. G., Jones, M., Davies, B., Peters, A. R., & Williams, S. P. (1996). Oestrus synchronization in ewes: the effect of combining a prostaglandin analogue with a GnRH agonist (buserelin). Animal Science, 62(1), 85-87.
7. Burke, C. R., Mussard, M. L., Grum, D. E., & Day, M. L. (2001). Effects of maturity of the potential ovulatory follicle on induction of oestrus and ovulation in cattle with oestradiol benzoate. Animal Reproduction Science, 66(3-4), 161-174. https://doi.org/10.1016/S0378-4320(01)00101-4.
8. Cavalieri, J., Rubio, I., Kinder, J. E., Entwistle, K. W., & Fitzpatrick, L. A. (1997). Synchronization of estrus and ovulation and associated endocrine changes in Bos indicus cows. Theriogenology, 47(4), 801-814.
9. Crosby, T. F., Boland, M. P., & Gordon, I. (1991). Effect of progestagen treatments on the incidence of oestrus and pregnancy rates in ewes. Animal Reproduction Science, 24(1-2), 109-118.
10. Didarkhah, M. (2018). A review of the methods Synchronization of estrus and stimulation of ovulation in ruminants. Journal of Biosafety, 10(4), 31-46. (In Persian).
11. Diskin, M. G., Austin, E. J., & Roche, J. F. (2002). Exogenous hormonal manipulation of ovarian activity in cattle. Domestic Animal Endocrinology, 23(1-2), 211-228.
12. Echternkamp, S. E. (1982). Influence of breed and season on ovarian and pituitary response in progestogen-PMSG-treated ewes. Theriogenology, 18(1), 95-106.
13. Eskandari, H., Keshtkaran, A. N., Meamar, M., & Habibizad, J., (2020). The effect of short term and long term feeding of barley seed with or without eCG on reproductive performance of goat during breeding and out breeding seasons. Research on Animal Production, 11(29), 153-161. (In Persian).
14. Evans, G., & Armstrong, D. T. (1984). Reduction of sperm transport in ewes by superovulation treatments. Reproduction, 70(1), 47-53.
15. Fonseca, J. F., Bruschi, J. H., Santos, I. C. C., Viana, J. H. M., & Magalhaes, A. C. M., (2005). Induction of estrus in non-lactating dairy goats with different estrous synchrony protocols. Animal Reproduction Science, 85(1-2), 117-124. https://doi.org/10.1016/j.anireprosci.2004.03.005
16. Garoussi, M. T., Farzaneh, N., Gallehdar, E., & Mohri, M. (2012). Reproductive performance in out-of-breeding season of fatty ewes using implant norgestomet with or without PMSG. Tropical Animal Health and Production, 44(5), 965-968. DOI 10.1007/s11250-011-9993-z.
17. Gonzalez-Bulnes, A., Santiago-Moreno, J., Garcia-Garcia, R. M., Del Campo, A., Gomez-Brunet, A., & Lopez-Sebastian, A. (2001). Origin of the preovulatory follicle in Mouflon sheep (Ovis gmelini musimon) and effect on growth of remaining follicles during the follicular phase of oestrous cycle. Animal Reproduction Science, 65(3-4), 265-272. https://doi.org/10.1016/S0378-4320(01)00076-8.
18. Gonzalez‐Bulnes, A., Garcia‐Garcia, R. M., Souza, C. J. H., Santiago‐Moreno, J., Lopez‐Sebastian, A., Cocero, M. J., & Baird, D. T. (2002). Patterns of follicular growth in superovulated sheep and influence on endocrine and ovarian response. Reproduction in Domestic Animals, 37(6), 357-361. https://doi.org/10.1046/j.1439-0531.2002.00385.x.
19. Gordon, I. R. (1997). Controlled reproduction in farm animal's series, vol. 2. Controlled reproduction in sheep and goats. New York: CAB International.
20. Habibizad, J., Tohidi, M., Meamar, M., & Alipoor, M. (2019). Reproductive performance changes in Lak and Torki Ghashghaei ewes by using eCG in nomadic breeding condition. Iranian Journal of Veterinary Clinical Sciences, 13(2), 43-55. (In Persian).
21. Haji Ghorbani, H., Tabatabaei, S. H., & Karimi, N. (2018). Effects of injection of PGF2α and eCG after CIDR removal on reproductive performance in Atabay ewes in non-breeding season. Journal of Animal Environment, 10(4), 91-98. (In Persian).
22. Hejazi, P., Masoumi, R., Shahmoradi, M., Rostami, B., & Bagherinia Amiri, M. (2018). Evaluation of reproductive performance of Afshari ewes with a short estrus synchronization program in nonbreeding season. Research on Animal Production, 9(21), 80-85. (In Persian). DOI: 10.29252/rap.9.21.80.
23. Iglesias, R. R., Ciccioli, N. H., Irazoqui, H., & Giglioli, C. (1996). Ovulation rate in ewes after single oral glucogenic dosage during a ram-induced follicular phase. Animal Reproduction Science, 44(4), 211-221.
24. Johnson, S. K., Dailey, R. A., Inskeep, E. K., & Lewis, P. E. (1996). Effect of peripheral concentrations of progesterone on follicular growth and fertility in ewes. Domestic Animal Endocrinology, 13(1), 69-79.
25. Joseph, I. B. J. K., Currie, W. D., & Rawlings, N. C. (1992). Effects of time after ovariectomy, season and oestradiol on luteinizing hormone and follicle-stimulating hormone secretion in ovariectomized ewes. Reproduction, 94(2), 511-523.
26. Kuru, M., Sogukpinar, O., Makav, M., & Cetin, N. (2017). Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during non-breeding season. Medycyna Weterynaryjna, 73(8), 479-482. DOI: 21521/mw.5758.
27. Kuru, M., Kükürt, A., Oral, H., & Öğün. M. (2018). Clinical use of progesterone and its relation to oxidative stress in ruminants. pp. 303–327 in Sex Hormones in Neurodegenerative Processes and Diseases.
28. Kuru, M., Kuru, B. B., Sogukpinar, O., Sen, C. C., Oral, H., & Kirmizibayrak, T. (2020). Oestrus synchronisation with progesterone-containing sponge and equine chorionic gonadotropin in Pirlak ewes during the non-breeding season: can Toryum improve fertility parameters? Journal of Veterinary Research, 64(4), 573-576. DOI:2478/jvetres-2020-0074.
29. Kusina, N. T., Tarwirei, F., Hamudikuwanda, H., Agumba, G., & Mukwena, J. (2000). A comparison of the effects of progesterone sponges and ear implants, PGF2 alpha, and their combination on efficacy of estrus synchronization and fertility of Mashona goat does. Theriogenology, 53(8), 1567-1580. https://doi.org/10.1016/S0093-691X(00)00298-3.
30. Leyva, V., Buckrell, B. C., & Walton, J. S. (1998). Regulation of follicular activity and ovulation in ewes by exogenous progestagen. Theriogenology, 50(3), 395-416.
31. Martemucci, G., Toteda, F., Facciolongo, A. M., D'Alessandro, A., & Gambacorta, M. (1997). Ovarian response, serum oestradiol-17beta concentration, and embryo yield in anestrus ewes treated with PMSG or two porcine gonadotrophin pituitary extracts. Zootecnica E Nutrizione Animale, 23, 81-88.
32. Martinez, M. F., McLeod, B., Tattersfield, G., Smaill, B., Quirke, L. D., & Juengel, J. L. (2015). Successful induction of oestrus, ovulation and pregnancy in adult ewes and ewe lambs out of the breeding season using a GnRH+progesterone oestrus synchronisation protocol. Animal Reproduction Science, 155, 28-35. https://doi.org/10.1016/j.anireprosci.2015.01.010.
33. Martinez-Ros, P., Astiz, S., Garcia-Rosello, E., Rios-Abellan, A., & Gonzalez-Bulnes, A. (2018). Effects of short-term intravaginal progestagens on the onset and features of estrus, preovulatory LH surge and ovulation in sheep. Animal Reproduction Science, 197, 317-323. https://doi.org/10.1016/j.anireprosci.2018.08.046.
34. Martinez-Ros, P., Rios-Abellan, A., & Gonzalez-Bulnes, A. (2019). Influence of progesterone-treatment length and eCG administration on appearance of estrous behavior, ovulatory success and fertility in sheep. Animals, 9(1), 9. https://doi.org/10.3390/ani9010009.
35. Masoodi, R., Kohram, , Lotfi, M., & Ghaffari, M. (2014). Evaluation of reproductive parameters in different programs of CIDR insertion and eCG injection in Mahabadi does during nonbreeding season. Iranian Veterinary Journal, 10(1), 96-102. (In Persian).
36. Menchaca, A., & Rubianes, E. (2004). New treatments associated with timed artificial insemination in small ruminants. Reproduction, Fertility and Development, 16(4), 403-413. https://doi.org/10.1071/RD04037.
37. Menchaca, A., dos Santos-Neto, P. C., Cuadro, F., Souza-Neves, M., & Crispo, M. (2018). From reproductive technologies to genome editing in small ruminants: An embryo’s journey. Animal Reproduction, 15(Supplement 1): 984-995. http://dx.doi.org/10.21451/1984-3143-AR2018-0022.
38. Neisi, Z., Mamouei, , Roshanfekr, H.,  Vakili Tabatabaei, S., & Sari, M. (2016). Comparison the pregnancy rates in different synchronization and ovulation protocols in Khuzestan buffalo. Journal of Animal Science Research, 26(1), 43-50. (In Persian).
39. Oliveira, M. E. F., Ayres, H., Oliveira, L. G. D., Barros, F. F. P. D. C., Oba, E., Bicudo, S. D., & Vicente, W. R. R. (2016). Effects of season and ovarian status on the outcome of long-term progesterone-based estrus synchronization protocols and ovulatory follicle development in Santa Ines ewes under subtropical conditions. Theriogenology, 85(3), 452-460. https://doi.org/10.1016/j.theriogenology.2015.09.024.
40. Öztürkler, Y., Çolak, A., Baykal, A., & Güven, B. (2003). Combined effect of a prostaglandin analogue and a progestagen treatment for 5 days on oestrus synchronization in Tushin ewes. Indian Veterinary Journal, 80, 917-920.
41. Ryan, J. P., Hunton, J. R., & Maxwell, W. M. (1991). Increased production of sheep embryos following superovulation of Merino ewes with a combination of pregnant mare serum gonadotropin and follicle stimulating hormone. Reproduction, Fertility and Development, 3(5), 551-560.
42. Sadeghi Panah, A., Masoudi, R., Naeejian, H. R., & Akbari-Sharif, A. (2015). Effect of eCG, PGF2α and GnRH hormones on ewes' reproductive performance in breeding season. Iranian Journal of Animal Science, 46(2), 189-194. (In Persian). https://doi.org/10.22059/ijas.2015.55650.
43. Sadeghi Panah, H. (2016). Induction and synchronization of estrus through hormone therapy. Publication of Agricultural Education, Agric. Handbook No. 95. (In Persian).
44. Sareminejad, P., Tabatabaei, S., Mamouei, M., Mirzadeh, K., & Boujarpour, M. (2014). The effects of short and long term medroxy progesterone acetate (MAP) sponge treatments on reproductive performance during the non-breeding season of arabian ewes. Iranian Journal of Applied Animal Science, 4(4), 747-751.
45. Swelum, A. A. A., Alowaimer, A. N., & Abouheif, M. A. (2015). Use of fluorogestone acetate sponges or controlled internal drug release for estrus synchronization in ewes: Effects of hormonal profiles and reproductive performance. Theriogenology, 84(4), 498-503. https://doi.org/10.1016/j.theriogenology.2015.03.018.
46. Takada, L., Bicudo, S. , Rodrigues, C. F. D. C., Coelho, L. D. A., Mendes, L. C. N., & Perri, S. H. V. (2012). Ovarian response of Suffolk ewes to estrous synchronization using short-term protocol. Revista Brasileira de Zootecnia, 41(2), 314-319. https://doi.org/10.1590/S1516-35982012000200012.
47. Tempest, W. M., & Minter, C. M. (1987). Synchronized breeding and lambing. pp. 221–237 in New Techniques in Sheep Production. Butterworth-Heinemann.
48. Tsonis, C. G., Cahill, L. P., Carson, R. S., & Findlay, J. K. (1984). Identification at the onset of luteolysis of follicles capable of ovulation in the ewe. Reproduction, 70(2), 609-614.
49. Ueno, D., Goto, A., Hazano, K., Haneda, S., & Matsui, M. (2021). Sustained high progesterone concentrations during estradiol-progesterone based estrus synchronization protocol in Japanese Black cows affects fertility by influencing preovulatory follicle size and its ovulation. Japanese Journal of Veterinary Research, 69(3), 151-161. DOI: 14943/jjvr.69.3.151.
50. Ustuner, B., Gunay, U., Nur, Z., & Ustuner, H. (2007). Effects of long and short-term progestagen treatments combined with PMSG on oestrus synchronization and fertility in Awassi ewes during the breeding season. Acta Veterinaria Brno, 76(3), 391-397. https://doi.org/10.2754/avb200776030391.
51. Ungerfeld, R., & Rubianes, E. (2002). Short term primings with different progestogen intravaginal devices (MAP, FGA and CIDR) for eCG-estrous induction in anestrus ewes. Small Ruminant Research, 46(1), 63-66. https://doi.org/10.1016/S0921-4488(02)00105-0.
52. Van Cleeff, J., Karsch, F. J., & Padmanabhan, V. (1998). Characterization of endocrine events during the periestrous period in sheep after estrous synchronization with controlled internal drug release (CIDR) device. Domestic Animal Endocrinology, 15(1), 23-34.
53. Viñoles, C., Forsberg, M., Banchero, G., & Rubianes, E. (2001). Effect of long-term and short-term progestagen treatment on follicular development and pregnancy rate in cyclic ewes. Theriogenology, 55(4), 993-1004. DOI: 1016/s0093-691x(01)00460-5 .
54. Viñoles, C., Paganoni, B., Milton, J. T. B., Driancourt, M. A., & Martin, G. B. (2011). Pregnancy rate and prolificacy after artificial insemination in ewes following synchronisation with prostaglandin, sponges, or sponges with bactericide. Animal Production Science, 51(6), 565-569. https://doi.org/10.1071/AN10200.