Investigation of bacterial contamination with Klebsilla and E. coli in the prepucal cavity of pubertal and adult age in caprine

Iranian Journal of Veterinary Science and Technology

دوره 15، شماره 3 - شماره پیاپی 32، آذر 2023، صفحه 27-32   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2023.82390.1249

نویسنده

Ansam Khalid Mohammed^*

Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.

چکیده

The herein research was carried out in order to identified the presence of bacteria inside prepuce cavity of male caprine in both mature and pubertal age with focusing on Klebsilla and E coli species. Eighty prepuce swabs (fifty form mature and thirty from pubertal age) before slaughtering and cultured on blood agar and nutrient agar, bacterial isolation were identified with biochemical teats and finally by PCR. The present study found a significant difference (p < 0.01) between the prepuce swabs from caprine mature age (64%) and pubertal age (40%). Six various microorganisms were detected in prepuce samples in mature age, while four types were isolated from pubertal age. Positive isolation swabs detected the presence of Staphylococcus aurues, Escherichia coli, Pseudomonas aeruginosa and Streptococcus faecalis in both ages. Proteus mirabilis and Klebsiella pneumonia was isolated from mature age only. Significant isolation (p < 0.01) was appear of Escherichia coli among all different bacterial types. This research deduce that there was that the presence of bacteria inside prepuce of male genital system in both mature and pubertal age and their where a balance between genital immunity and localization of these bacteria and any stress factor may be lead to infection with such microbes, more over the mature male had more bacterial types due to the male matting behavior, finally the E. coli normally found in prepuce cavity as a normal flora of both ages and the Klebsilla species also found in mature age as a non-specific bacterial types.

کلیدواژه‌ها

Klebsiella؛ Escherichia coli؛ Prepucal cavity؛ Pubertal age؛ Adult age؛ Goat

سایر فایل های مرتبط با مقاله

IJVST-15-27.xml

مراجع

1.    Al-Delemi DHJ, Karam KM, Jafar AM. The normal bacterial flora in the prepucal cavity of Iraqi bulls, rams and bucks. Al-Anbar J Vet Sci. 2009; 1(2): 74-81. 
2.    Aziz DM, Al-Sultan MAH, Al-Jawally EAK. Uterine microflora in Awassi ewes. Iraqi J Vet Sci. 2002; 13: 201-205. 
3.    Al-Hilali HA, Al-Delemi DH. The uterine bacterial flora of normal reproductive tract, non-pregnant Iraqi cows. The Veterinarian. 2001; 11(1): 112-120. 
4.    Al-Delimi DHJ. The uterine bacterial flora of reproductive tract of the Iraqi she-camels. Al-Qadisiya J Vet Med Sci. 2002; 1(2): 55-59.
5.    Al-Delemi DHJ. The normal bacterial flora in the vaginal cavity of Iraqi cows, sheep, goats and camels during the luteal phase. Al-Qadisiya J Vet Med Sci. 2005; 4: 23-29. 
6.    Al-Badry KI, Al-Jobori AH, Ibrahim FF. Influence of tylosin, gentamicin and lincomycin combination on semen characteristics, bacterial and mycoplasma contaminants of Friesian bull semen. Iraqi J Microbiol. 2001; 13(3): 18-28.
7.    Troedsson MH, Loiset K, Alghamdi AM, Dahms B, Grabo BG. Interaction between equine semen and the endometrium: The inflammatory responses to semen. Ani RepSci. 2001; 68: 273-278. Doi: 10.1016/s0378-4320(01)00164-6.
8.    Causey RC. Making sense of equine uterine infections: the many faces of physical clearance. Vet J. 2006; 172: 405-421. Doi: 10.1016/j.tvjl.2005.08.005
9.    Ahmed MN, Ynzeel JH, Majeed AF. Bacterial study of vagina in Awassi ewes treated with Prostaglandin (PGF2 α) and oxytocin. Al-Anbar J Vet Sci. 2017; 10(1): 96-99. 
10.    Yaseen SM, Saleh AM, Al-Zubaidy RSA. Contamination of the local produced broilers carcasses with Escherichia coli O157: H7 and its effect in public health in Diyala province. The Iraqi Journal of Veterinary Medicine. 2018; 41(2): 113–117. 
11.    Al-Taii DHF, Yousif AA. Effects of E. coli O157:H7 Experimental Infections on Rabbits. The Iraqi Journal of Veterinary Medicine. 2019; 43(1): 34–42. Doi:10.30539/iraqijvm.v43i1.468
12.    Hanoun ATT, Al-Samrraae IAA. Isolation and Identification of Escherichia coli and Salmonella typhimurium from Sheep in Baghdad city. The Iraqi Journal of Veterinary Medicine. 2019; 43(1): 124–129.Doi:10.30539/iraqijvm.v43i1.482
13.    Bakheet NZ, Al-Qayim MAJ, Falih IB. Intestinal Anti-Inflammatory Improvement with Fenugreek Seeds as A prebiotic and Synbiotic with Lactobacillus acidophilus in Rats Experimentally Infected with Escherichia coli. The Iraqi Journal of Veterinary Medicine. 2020; 44(2): 1–14. Doi:10.30539/ijvm.v44i2.984
14.    Zaid NW, Abdalla AH, Ibrahim AH, Khadim AA. Effect of prostoglandine PGF2α on bacterial elimination during puerperium in Awassi ewes. Al-Anbar J Agricul Sci. 2007; 5(1): 211-217. 10.32649/ajas.2007.36264
15.    Zaid NW, Al-Zubaidy IA. The Effect of Natural Mating on the Bacterial Pollution in the Endogenous Ram. Al-Anbar J Agricul Sci. 2009; 2(1): 31-35. 
16.    Zaid NW, Al-Samarrae EAA, Laiby AK. Bacterial isolation before and after natural mating of endogenous bucks. Al-Qadisiya J Vet Med Sci. 2010; 9(1): 16-19. 
17.    Marinov P, Hummel P, Bohnel H. Observations on the microbial content in bull semen and vitality of bovine spermatozoa in presence of certain microorganisms. 7th International Congress on Animal Reproduction and Artificial Insemination - Munich II: 1569-1575. 1972.
18.    Pycock JF. How to maximize the chances of breeding successfully from the older mainden mare. Proceeding American Association of Equine Practitioners. 2006; 52: 245-249. 
19.    Zerbe H, Ossadnik C, Leibold W, Schuberth HJ. Influence of Escherichia coli and Arcanobacterium pyogenes isolated from bovine puerperal uteri on phenotypic and functional properties of neutrophils. Vet Microbiol Approach. 2001; 79 (4): 351-352. Doi: 10.1016/s0378-1135(00)00368-0.
20.    Zerbe H, Ossadnik C, Leibold W, Schuberth HJ. Influence of Escherichia coli and Arcanobacterium pyogenes isolated from bovine puerperal uteri on phenotypic and functional properties of neutrophils. Vet Microbiol Approach. 2001; 79 (4): 351-352. Doi: 10.1016/s0378-1135(00)00368-0.
21.    Rigby SL, Barhoumi R, Burghardt RC, Colleran P, Thompson JA, Varner DD, Blanchard TL, Brinsk SP, Taylor T, Wilkerson MK, Delp MD. Mares with delayed uterine clearance have an intrinsic defect in myometrial function. Biol Reprod. 2001; 65: 740-747. Doi: 10.1095/biolreprod65.3.740.
22.    Card C. Post-breeding inflammation and endometrial cytology in mares. Theriogenology. 2005; 64: 580-588. Doi: 10.1016/j.theriogenology.2005.05.041.
23.    Al-Zubaidi SFA, Abdul-Hussain ZB, Abdul-Hussain IF. Vaginal microflora in ewes after estrus synchronization with intravaginal sponges. The Iraqi Journal of Veterinary Medicine. 2018; 41(2): 67–71. Doi:10.30539/iraqijvm.v41i2.50
24.    Al-Delemi DHJ. Anatomical, physiological, bacteriological and pathological study of the reproductive system of Iraqi she-camels (Camelus dromedaries). Thesis of philosophy degree in theriogenology and genital diseases – veterinary medicine college – Baghdad university. 2007.
25.    Forbes BA, Sahm DF, Weissfeld AS. Bailey and Scotts' Diagnostic Microbiology. 12th ed. Elsevier. 2007. ISBN: 9780323681056
26.    Sneath PA, Mair NS, Sharp ME, Hott JG. Bergey's Manual of systematic Bacteriology. William and Wilkins, USA. 1986. https://www.springer.com/series/4157
27.    Schippa S, Lebba V, Barbaro M, Nardo GD, Totino V, Checchi MP, Longhi C, Maiella G, Cucchiara S, Conte MP. A distinctive microbial signature' in celiac pediatric patients. BMC Microbiology. 2010; 10: 175. Doi:10.1186/1471-2180-10-175.
28.    Abd Alwahed BA, Latteef NS, Al-Daraghi WAH. Molecular Detection Of Klebsiella Pneumoniae Isolated From Different Clinical sources. Sys Rev Pharm. 2021; 12(1): 931-934. 
29.    SAS. Statistical Analysis System, Usre's Guide. Statistical Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA. 2012.