اثر ترکیبی عصاره سیر (Allium sativum)و زردچوبه (Curcuma longa) بر کاهش اثرات سوء ناشی از آفلاتوکسین B1 در موش‌های صحرایی

پژوهشهای علوم دامی ایران

مقاله 8، دوره 16، شماره 4 - شماره پیاپی 60، دی 1403، صفحه 555-568   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.22067/ijasr.2024.83186.1157

نویسندگان

هادی سریر^{* 1}؛ سعیده ذاکریان¹؛ مسعود دیدارخواه²

¹گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

²آموزشکده کشاورزی سرایان، دانشگاه بیرجند، بیرجند، ایران

چکیده

این مطالعه به‌منظور ارزیابی اثر هم‌افزایی عصاره سیر و زردچوبه در برابر آسیب ناشی از آفلاتوکسین B₁ روی 64 سر موش صحرایی نر 10-12 هفته، با میانگین وزنی 300-250 گرم در قالب یک طرح کاملاً تصادفی به‌صورت آزمایش فاکتوریل (2 × 2 × 2) در هشت گروه و به‌مدت 28 روز انجام شد. تیمارهای آزمایشی شامل: 1- شاهد (2/0 میلی‏لیتر آب مقطر استریل به‌صورت خوراکی)، 2- آفلاتوکسین B₁ (100 میکروگرم به‌ازای هر کیلوگرم وزن بدن)، 3- عصاره سیر (50 میلی‏گرم به‌ازای هر کیلوگرم وزن بدن)، 4- عصاره زردچوبه (100 میلی‏گرم به‌ازای هر کیلوگرم وزن بدن)، 5- عصاره سیر همراه با عصاره زردچوبه، 6- آفلاتوکسین B₁ همراه با عصاره زردچوبه، 7- آفلاتوکسین B₁ همراه با عصاره سیر، 8- آفلاتوکسین B₁ همراه با عصاره سیر و زردچوبه بودند. در تمام گروه‌ها، آفلاتوکسین و عصاره‌‌ها به‌صورت خوراکی داده شد. نتایج نشان داد که استفاده از عصاره زردچوبه، سیر و ترکیب آن‏ها بدون آفلاتوکسین اثر معنی­داری بر فراسنجه‌های اندازه‏گیری شده نداشت. آفلاتوکسین B₁ موجب افزایش معنی­دار آنزیم‌های کبدی AST (Aspartate aminotransferase)، ALT (Alanine transaminase)، ALP (Alkaline phosphatase)، بیلی‌روبین تام، مالون‏دی‏آلدهید و کاهش معنی­دار ظرفیت آنتی‏اکسیدانی تام، T₃ (تری یدوتیرونین) و T₄ (تیروکسین) نسبت به گروه شاهد دارای آفلاتوکسین شد. نتایج این تحقیق نشان داد که عصاره سیر و زردچوبه اثر بالقوه­ای بر کاهش سطوح ALP، ALT و افزایش سطح T₄ در مقایسه با گروه‏های مسموم شده دریافت‌کننده عصاره سیر یا زردچوبه به‌تنهایی دارد.

کلیدواژه‌ها

آفلاتوکسین B1؛ زردچوبه؛ سیر؛ مالون‏دی‏آلدهید؛ هورمون‏های تیروئیدی

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