Eremurus spectabilis Root Extract: Evaluating Different Extraction Methods and Antimicrobial and Antioxidant Characteristics

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 30 دی 1403   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2025.88938.1396

نویسندگان

Elham Merrikhi Ardebili¹؛ Abdollah Jamshidi^{* 1}؛ Razieh Niazmand²

¹Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

²Department of Food Chemistry, Research Institute of Food Science and Technology, Mashhad, Iran

چکیده

Abstract
The current study aims to explore the potential antimicrobial and antioxidant activities of Eremurus spectabilis (E. spectabilis) in 3 extract techniques. Three methods were selected to extract E. spectabilis, aqueous extract, alcoholic extract, and hydroalcoholic extract. The extraction yield was obtained from 10 grams of E. spectabilis powder. The carbohydrate test was performed using the phenol sulfuric acid method. The Kjeldahl method was used in two replicates based on the AOAC 2550 standard to determine the protein content. The concentration of phenolic compounds was measured by the Folin-Ciocalteu assay. Based on the results, E. spectabilis had 70.33 g/100g of carbohydrates and 7.1 g/100g proteins. The extraction percentages for the aqueous, alcoholic, and hydroalcoholic extracts of E. spectabilis were 50%, 10%, and 25%, respectively. The results showed that the aqueous extraction method was the most efficient. The total phenol amount for E. spectabilis aqueous extract was 150.04 mg/g. The antioxidant properties of E. spectabilis aqueous extract were equal to 50.71. All concentrations of aqueous extract did not have antimicrobial properties against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Conclusion- These findings underscore the need for further exploration, using different concentrations, and evaluating other pathogens.

کلیدواژه‌ها

Antimicrobial؛ Antioxidant؛ Eremurus spectabilis, Hydroalcoholic extraction؛ Alcoholic extraction