TTILE: BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF Pseudomonas putida, P. fluorescens and P. aeruginosa ISOLATES FROM Oreochromis niloticus (TILAPIA ) AND Clarias gariepinus (AFRICAN CATFISH)

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 10 خرداد 1404   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2025.90677.1437

نویسندگان

Mannir Dahiru Usman^{* 1}؛ Clarance Suh YAH²؛ Victoria Folakemi Akinjogunla³؛ Tuaheed Abubakar Muazu⁴؛ Abdulrazak Lawal⁵؛ Yusuf Abubakar⁵؛ Khalid Shuaibu HASSAN⁶؛ Jamiu Olatoye Salako⁴؛ Christian Anuoluwapo OLASENI⁷

¹Department of Veterinary Medicine, Bayero University Kano Nigeria

²2. Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa

³3. Department of Fisheries and Aquaculture, Bayero University Kano, Nigeria

⁴4. Department of Veterinary Anatomy, Bayero University Kano, Nigeria

⁵5. Department of Veterinary Public health and Preventive Medicine, Bayero University Kano, Nigeria

⁶6. Centre for Dryland Agriculture, Bayero University Kano

⁷3. Department of Fisheries and Aquaculture, Bayero University Kano, Nigeria

چکیده

ABSTRACT

Bacterial pathogens cause high economic losses in the fish farming industry and seriously threaten public health. This study described the phenotypic and the genomic characteristics of pathogenic Pseudomonas species in two finfishes (Oreochromis niloticus and Clarias gariepinus) from the Galadima fish market in Kano metropolis Nigeria. Twenty fish samples, including 10 Tilapia and 10 African catfish were randomly obtained and their liver, spleen, intestine and gills were collected. Pseudomonas spp. was screened via culturing and isolation techniques and biochemical tests. The molecular characterization of the isolates was carried out based on 16S rRNA gene sequence analysis. A total of 6 % Pseudomonas spp. isolates were confirmed as P. putida (5 %), P. aeruginosa (1.25 %), and P. fluorescens (1.25 %). This study confirms the presence of potential pathogenic Pseudomonas species in commercially important finfish species from the Galadima fish market in Kano, Nigeria. These findings highlight the need for regular monitoring and molecular surveillance of bacterial pathogens in aquaculture to mitigate economic losses and reduce potential public health risks associated with fish consumption.

کلیدواژه‌ها

Tilapia؛ African catfish؛ Sequencing؛ Bacteria