Stable Overexpression of MZF1 Does Not Alter Proliferation, Viability, or Stemness in HEK293T Cells

Journal of Cell and Molecular Research

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 20 مرداد 1404

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2025.93826.1113

نویسندگان

Zahra Hosseininia¹؛ Hesam Dehghani^{* 2}

¹1. Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 2. Stem Cells and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

²1. Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 2. Stem Cells and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad,

چکیده

Myeloid Zinc Finger 1 (MZF1) is a member of the SCAN domain-zinc finger (SCAN-ZFP) transcription factor family. With a structure comprising five distinct domains, it regulates various transcriptional processes. Numerous studies suggest that MZF1 has bi-functional roles, acting either as an oncogene or a tumor suppressor. However, its overexpression has also been associated with negligible changes in cellular functions, an aspect that remains underexplored. Additionally, although the HEK293T cell line is widely used to investigate molecular mechanisms, the role of MZF1 in these cells has not been reported. In this study, we assessed the effects of MZF1 overexpression on some cellular functions in these cells. Using transposon-mediated gene transfer, we generated stably expressing cell lines and assessed their cellular functions, including cell proliferation, cell viability, and cell stemness. Our findings show that following stable expression of MZF1, the number of cells compared to controls did not reveal a significant difference during 6 time-points over 72 hours. Also, the MTT read absorbance and as a consequence, cell viability did not change in the MZF1 overexpressing cell line compared to control cells (0.49 against 0.48). By performing the colony formation assay, we did not find any significant changes in the number of colonies or the number of cells in each colony. Also, our soft agar assay did not reveal statistically significant changes in the size and number of colonies between the two groups of MZF1-overexpressing cells and control cells. Taken together, our data provide evidence that the stable overexpression of MZF1 does not alter cellular functions of proliferation, viability, and stemness in HEK293T cells.

کلیدواژه‌ها

MZF1؛ HEK293T cell line؛ cell proliferation؛ cell viability؛ cell stemness