Investigating the effect of nano-lactoferrin on cytotoxicity and expression of autophagy genes Beclin 1, P53 and LC3I in breast cancer cell line MCF7 and normal cell line Vero

Journal of Cell and Molecular Research

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 25 شهریور 1404

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2025.89162.1096

نویسندگان

Fatemeh Moradian^{* 1}؛ Najibeh Akbari Nowzari¹؛ Ayoub Farhadi²

¹Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University

²Department of Animal Sciences, Faculty of Animal and Fishery Sciences, Sari Agricultural Sciences and Natural Resources University

چکیده

Autophagy can play an inhibitory role in the early stages of cancer, so studying autophagy genes can help with therapeutic strategies. This study investigated the effect of lactoferrin (Lf) and nano-encapsulated Lf (NLf) on the expression levels of autophagy-inducing genes Beclin 1, p53 and LC3I. Also, the effect of Lf and NLf on the growth rate of breast cancer cells MCF7 and normal cells Vero was studied. The results showed that the survival percentage of MCF7 cells in nano-lactoferrin (NLf) treatment was lower than Lf with the same concentrations. The percentage of survival in Vero cells treated with NLf and Lf was higher than that of cancer cells, and cell death was not significant in this normal cell line. The level of the Beclin 1 gene expression in concentrations of 200 and 300 µg of NLf and Lf increased significantly compared to the control in cancer cells. The expression levels of p53 and LC3I genes were significantly increased in all concentrations of NLf and Lf compared to the control in cancer cells. The expression levels of all three genes in different concentrations of NLf in normal cells showed a significant increase. The level of gene expression and cell death by NLf was slightly higher than that of Lf in cancer cells, which suggests that the intracellular penetration of NLf was greater than Lf. Lf causes cell survival and protects cells during stress by inducing autophagy in normal cells and removing damaged organelles, and causes cell death in cancer cells in the early stages.

کلیدواژه‌ها

Autophagy؛ Biological drug؛ Breast cancer؛ Gene expression؛ Nano-lactoferrin