Evaluation of Egg Drop Syndrome Virus Fiber Protein as a Vaccine Candidate: In Silico Analysis, Expression, Purification and Its Stability

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 09 مهر 1404

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2025.93948.1564

نویسندگان

Javad Najafian¹؛ Abasalt Hosseinzadeh Colagar^{* 2}؛ Ali Mostafaie³

¹Department of Molecular and Cell Biology, Faculty of Science, University of Mazandaran, Babolsar

²Department of Molecular and Cell Biology, Faculty of Science, University of Mazandaran, Babolsar, PC: 47416-95447, Mazandaran, Iran.

³Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

چکیده

The egg drop syndrome virus (EDSV), an avian adenovirus, triggers a sharp decline in both egg production and quality in infected chickens, resulting in significant economic impacts for the poultry sector. Based on previous studies, EDSV fiber protein could be a candidate for subunit vaccine against EDSV. Present research focuses on the expression, purification, and thermal stability evaluation of recombinant fiber protein as a vaccine against EDSV. In an in silico approach, we investigated the fiber protein structure and expressed it in Escherichia coli (E. coli) as a vaccine candidate. We also evaluated the thermal stability of the expressed protein. The protein was expressed predominantly as soluble trimeric proteins in E. coli and expression level after nickel-affinity purification was 15 mg/L. Structural analysis using immunological and bioinformatics tools Confirmed retention of the native trimeric conformation in the recombinant protein. Based on the thermal stability evaluation on this recombinant protein, the protein showed good thermal stability, which can be a good candidate for a vaccine against EDSV.

کلیدواژه‌ها

Egg drops syndrome virus؛ Fiber protein؛ In silico؛ Vaccines