Characterization and phylogenetic analysis of a mitochondrial targeting alpha/beta hydrolase protein in Strongyloides ratti

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 27 آبان 1404

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2025.93653.1549

نویسنده

Abbas Jolodar^*

Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

چکیده

Strongyloides ratti serves as an experimental model for diagnosing strongyloidiasis in humans. The alpha/beta hydrolase domain proteins are ubiquitously distributed across all organisms. A 780-nucleotide cDNA fragment was amplified by RT-PCR, and a GenBank search revealed that the amplified products were 98.54% identical to the only incomplete mRNA sequence available for S. ratti (XM_024652394.1). Using the amplified gene fragment as a probe, two sequences of 856 bp (FC815910.1) and 526 bp (BI742498.1), corresponding to the 3′ and 5′ ends, respectively, were identified through the expressed sequence tag (EST) database search. The assembled cDNA, spanning 919 bp, was designated SrABH. Its amino acid sequence exhibited 97.98% identity with the only available S. ratti sequence in GenBank (XP_024505975.1). The molecular weight (MW) and isoelectric point (pI) of SrABH were determined to be 33.484 kDa and 7.18, respectively. Notably, SrABH lacks a signal peptide, although a cleavage site for mitochondrial targeting was identified between amino acids 21-22. The highly conserved region consists of nine amino acids spanning positions 111 to 120. The 3D structural analysis revealed that SrABH consists of 9 alpha-helices (39.18%), 8 beta-sheets (18.21%), and loops (42.61%). Phylogenetic analysis showed that SrABH clusters with related nematode sequences, displaying a strong bootstrap support of 98. The lowest genetic distance (0.01%) was observed between SrABH and the only sequence isolated from S. ratti (XP_024505975.1). Given its molecular characteristics, this parasite’s data may be valuable in structure-based drug design strategies using S. ratti as a model system for the human pathogen S. stercoralis.

کلیدواژه‌ها

Esterase؛ alpha/beta hydrolase domain؛ Strongyloides ratti؛ RT-PCR؛ mitochondria