Cryo-infarction: a method of choice for the establishment of a rat model of myocardial infarction

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 08 آذر 1404   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2025.93330.1533

نویسندگان

Alireza Karimi¹؛ Maryam M Matin¹؛ Azadeh Haghighitalab¹؛ Hossein Kazemi Mehrjerdi²؛ Masoud Rajabioun³؛ Mehrdad Aghasizadeh⁴؛ Ahmad Reza Bahrami^{* 5}

¹Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Khorasan Razavi, Iran

²Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

³Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad.

⁴Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Khorasan Razavi, Iran.

⁵Ferdowsi University

چکیده

Ligation of the left anterior descending (LAD) coronary artery has been commonly employed to induce myocardial infarction (MI) in animals; however, this method is accompanied by a high mortality rate, especially in smaller laboratory animals due to induced bleeding and unpredictable areas of necrotic damage. Cryo-infarction is an alternate method that has been adopted to create a reproducible model of a myocardial injury while it increases the survival rate of the animal models. Ventilated male Wistar rats underwent open chest surgery followed by either coronary artery ligation or cryo-induced heart infarction. To validate the MI model, we conducted TTC and immunofluorescence staining, evaluated serum troponin levels, and analyzed Electrocardiography (ECG) data. additionally, echocardiography, hematoxylin and eosin staining, and Masson's trichrome staining were employed to assess the severity of MI. The Cryo method of inducing myocardial infarction offers several advantages including simplicity, reproducibility, and lower mortality rate. Since there are limited numbers of publications applying the method specially in rats, further comparative research is necessary to ascertain whether it accurately replicates the distinct characteristics of myocardial infarction induced by the LAD method. The application of the superior method for induction of myocardial infarction is of special importance to mimic the exact cell and molecular events encountered with myocardial infarction. This will benefit both basic and translational research in the field.

کلیدواژه‌ها

Heart؛ Myocardial infarction؛ Cryo-infarction؛ LAD؛ Rat model