Effect of cobalt nano-particles on serum biochemical and histopathological changes in liver and kidney of lambs

Iranian Journal of Veterinary Science and Technology

مقاله 2، دوره 5، شماره 2، 2013، صفحه 1-8   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v5i2.16589

نویسندگان

Seyed Morteza Ghoreishi¹؛ Hossein Najafzadeh^{* 1}؛ Babak Mohammadian¹؛ Eisa Rahimi²؛ Mohammad Reza Afzalzadeh³؛ Mohammad Kazemi Varnamkhasti¹؛ Hadi Ganjeali Darani,¹

¹Shahid Chamran University of Ahvaz

²Payame Noor University, Tehran

³Faculty of Shahid Chamran University of Ahvaz

چکیده

Cobalt is an essential co-factor in red blood cell production and function and its deficiency may produce clinical signs in sheep. Thus, present study was designed to evaluate the effect of cobalt nano-particles on serum biochemical factors and histopathological changes in liver and kidnies of lambs. Study was carried out in 3 groups of lambs (4 lambs per group). One
group of lambs was kept as control group. Second and third group respectively received cobalt nano-particles and conventional cobalt chloride suspension daily for a period 25 day.
Blood sample and then serum was collected before and at the end of study. Activity of ALT,AST, ALP and level of BUN, creatinine and vitamin B12 were measured in serum of lambs.
Tissue sections of liver and kidney were stained with hematoxylin and eosin and examined by light microscopy. Activity of ALT, BUN and vitamin B12 was significantly increased by cobalt nano-particles and conventional cobalt chloride. Fatty change of hepatocytes occured by conventional cobalt and granulomatous hepatitis, focal necrosis of hepatocytes and degeneration of hepatocytes by the nano cobalt was identified in liver. There were not any significant lesions and alteration in the kidnies of treated groups. Thus, cobalt nano-particles have similar effect to conventional cobalt for using in sheep with cobalt deficiency.

کلیدواژه‌ها

Cobalt nano-particles؛ hepatotoxicity؛ renal toxicity؛ lamb

مراجع