Study the effect of salinity levels and seed priming on germination and seedling properties of two medicinal plant species from Asteraceae family

بوم شناسی کشاورزی

Article 5, Volume 2, Issue 2 - Serial Number 4, June 2010, Pages 245-255   

Document Type: Scientific - Research

DOI: 10.22067/jag.v2i2.7630

Authors

Mohammad Kafi; Ehsan Eyshi Rezaei^* ; Matin Haghighi Khah; Sadegh Ghorbani

Department of Agronomy, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Soil and water sources salinity are important constrains which threat the sustainable agriculture production in Iran. In order to evaluate the effect of different antioxidants and salinity levels on germination and seedling properties of two medicinal species (Cnicus benedictus L.), and (Cichorium intybus L.), an experiment was conducted using a factorial based on completely randomized design with four replications at Special Crops Laboratory of Ferdowsi University of Mashhad. The studied factor for each plant included: seed priming at 4 levels including control (distilled water), ascorbic acid (40 mM), gibberlic acid (75 mg.lit-1) and salicylic acid (1.5 mM), and five salinity levels according to electrical conductivity by adding NaCl to distilled water (control, 5,10,15 and 20 ds.m-1). According to results, pretreatment with salicylic acid improved all of the germination and seedling properties in Cnicus benedictus L. but gibberlic acid could to improved germination and seedling properties in Cichorium intybus L. species. There were strong correlation between germination rate and radical and caulicle length especially on Cichorium intybus species. Generally, seed priming with gibberlic acid and salicylic acid could improve germination and seedling properties of these two species. Both species showed a reliable tolerance to NaCl salinity at germination stage, and germination was 60% compared with control at 20 ds.m-1 treatment.

Keywords

Antioxidant; Germination rate; Medicinal plants; Pretreatment

References

1- Anvari, M., Mehdikhani, H., Shahriari, A.R., and Nouri, G.R. 2009. Effect of salinity stress on 7 species of range plants in germination stage. Iranian Journal of Range and Desert Reseach 16(2): 262-273. (In Persian with English Summary).

2- Amor, N.B., Hamed, K.B., Debez, A., Grignon, C., and Abdelly, C. 2005. Physiological and antioxidant responses of the perennial halophyte Crithmum maritimum to salinity. Plant Science 168: 889–899.

3- Ashraf, M., Athar, H.R., Harris, P.J.C., and Kwon, T.R. 2008. Some prospective strategies for improving crop salt tolerance. Advanced in Agronomy 97: 45-92.

4- Baes, P.O., and Arechiga, M.R. 2007. Seed germination of Trichocereus terscheckii (Cactaceae): light, temperature and gibberellic acid effects. Journal of Arid Environment 69: 169-176.

5- Canakci, S., and Munzuroglu, O. 2007. Effects of acetylsalicylic acid on germination, growth and chlorophyll amounts of cucumber (Cucumis sativa L.) seeds. Pakistan Journal of Biological Science 17: 2930-2934.

6- Cho, U., and Park, J. 2000. Mercury–induced oxidative stress in tomato seedlings. International Journal of Plant Science 156: 1–9.

7- Dat, J.F., Lopez-Delgado, H., Foyer, C.H., and Scott, I.M. 1998. Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings. Plant Physiology 116:1351-1357.

8- Doulatabadian, A., Sanavy, A.M., and Etemadi, F. 2008. Effect of Pretreatment of Salicylic acid on Wheat (Triticum aestivum L.) Seed Germination under Salt Stress. Iranian Biology Journal 4: 692-702. (In Persian with English Summary).

9- Duros, L.M., and Magne, C. 2008. Effect of salinity and chemical factors on seed germination in the halophyte Crithmum maritimum. Plant and Soil 313:83-87.

10- Foley, M.E., and Fennimore, S.A. 1998. Genetic basis for seed dormancy. Seed Science Research 8: 173-179.

11- Foyer, C.H., Alschei, R.C., and Hess, J.L. 1993. Ascorbic Acid: a Antioxidants in Higher Plants. pp. 31–58. CRC Press, Boca Raton.

12- Gautam, S., and Singh, P.K. 2009. Salicylic acid-induced salinity tolerance in corn grown under NaCl stress. Acta Physiologiae Plantarum 31: 1185-1190.

13- Hagighi, R.S., and Milani, M.S. 2009. Osmotic and specific ion effects on the seed germination of Isabgol and Psyllium. Journal of Iranian Field Crop Research 7(1): 97-104. (In Persian with English Summary).

14- Janda, T., Szalai, G., Tari, I. and Paldi, E. 1999. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Planta 208: 175-180.

15- Jamil, M., Lee, D.B., Jung, K.Y., Ashraf, M., Lee S.C., and Rhal, E.S. 2006. Effect of salt (NaCl) stress on germination and early seedling growth of four vegetables species. Journal of Central European Agriculture 7:273–282.

16- Khan, M.A., and Ungar, I.A. 2001. Seed germination of Triglochin maritime as influenced by salinity and dormancy relieving compounds. Journal of Biological Plant 44: 301-307.

17- Khan, A.M., Ahmed, M.Z., and Hameed, A. 2006. Effect of sea salt and L-ascorbic acid on the seed germination of halophytes. Journal of Arid Environment 67: 535-540.

18- Kim, S.G., and Park, C.M. 2008. Gibberellic acid-mediated salt signaling in seed germination. Plant Signal Behav 3: 877-879.

19- Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science 2: 176-177.

20- Nakano, Y., and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxides in spinach chloroplasts. Plant Cell Physiology 22: 867–880.

21- Nadjafi, F., Bannayan, M., Tabrizi, L., and Rastgoo, M. 2006. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. Journal of Arid Environment 64: 542-547.

22- Norbek, R., Nielsen, K., and Kondo, T. 2002. Anthocyanins from flowers of Cichorium intybus. Phytochemistry 60: 357-359.

23- Omid Beyge, R. 2005. Production and Proccecing of Medicenal Plants. Beh Nashe Publisher. Pp. 70. (In persion)

24- Rajabian, T., Saboora, A., Hassani, B., and Hosseini, H.F. 2007. Effects of GA3 and chilling on seed germination of Ferula assa-foetida, as a medicinal plant. Iranian Journal of Medicinal and Aromatic Plants 23(3): 391-404. (In Persian with English Summary).

25- Safarnejad, A., Sadr, S.V.A., and Hamidi, H. 2007. Effect of salinity stress on morphological characters of Nigella sativa. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 15:75-84. (In Persian with English Summary).

26- Schopfer, P., Plachy, C., and Frahry, G. 2001. Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiology 125: 1591-1602.

27- Shah, S.H. 2007. Effects of salt stress on mustard as affected by gibberellic acid application. Genetic Application on Plant Physiology 33: 97-106.

28- Shiri, A.R.M., Safarnejad, A., and Hamidi, H. 2009. Morphological and biochemical characterization of Ferula assafoetida in response to salt stress. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 17: 38-49. (In Persian with English Summary).

29- Szepesi, Á., Csiszar, J., Bajkan, S., Gemes, K., and Horvath, F. 2005. Role of salicylic acid pre-treatment on the acclimation of tomato plants to salt- and osmotic stress. Acta Biologica Szegediensis 49: 123-125.

30- Yalpani N., Enyedi A.J., Leon, J., and Raskin, I. 1994. Ultraviolet light and ozone stimulate accumulation of salicylic acid and pathogenesis-related proteins and virus resistance in tobacco. Planta 193: 373-376.