In vitro Contamination Controlling of the Rhizome Explants of Alstroemeria Plant

علوم باغبانی

Article 14, Volume 25, Issue 1, September 2011   

Document Type: Research Article

DOI: 10.22067/jhorts4.v1390i1.9756

Authors

Amirghaffar Ghaffar Shahriari^* ; Abdolreza Bagheri; Ahmad Sharifi; Nasrin Moshtaghi

Abstract

Abstract
Alstroemeria is one of the most important cut flowers in the world which is multiplicated by rhizome explants in in vitro conditions . The major problem in using rhizome explants is fungal and bacterial contamination which inhibit the preparation of strill explants. Therefor in this experiment, the effect of disinfecting compounds like sodium hypochlorite, mercury chloride, nano silver particles, the antibiotics of streptomycin, penicillin, cefotaxime and carbendazim fungicide were examined on controlling of contamination. After disinfection, the explants were cultured on MS medium supplemented with 2 mg/L BA and 0.2 mg/L NAA. Contamination percentage was measured after three weeks. The results showed that the treatment of plants with 0.4% carbendazim fungicide and disinfection by 70% ethanol for one minute and %3 sodium hypochlorite for 20 minutes for caralis cultivar, and disinfection by 70% ethanol for one minute and %3 sodium hypochlorite for 20 minutes follow by culturing in medium with 200 mg/l either of streptomycin and penicillin in Bordeaux cultivar had the lowest contamination percentage. Contamination was not controlled by nano silver particles in studied plants.

Keywords: Alstroemeria, sodium hypoclorite, mercury chloride, antibiotics, carbendazim