Effects of Some Alpha-Amylase Inhibitors on the Salivary Alpha-Amylase Activity of the Stripped Bug, Graphosoma lineatum (Heteroptera: Scutelleridae)

پژوهش های حفاظت گیاهان ایران

Article 8, Volume 24, Issue 2 - Serial Number 8, June 2010   

Document Type: Research Article

DOI: 10.22067/jpp.v24i2.4134

Authors

Mohsen Yazdanian^* ; reza Farshbaf Pour Abad; Mohammadreza Rashidi; Mostafa Valizadeh; Nadereh Rashtchi Zadeh; Amirmansour Vatankhah; Aliasghar Hamidi

Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

Abstract
Digestive enzyme inhibitors are proteinacious or nonproteinacious compounds which reduce an enzyme activity through attaching to its active site and/or its substrate. Nowadays, plant enzyme inhibitors are of great importance because 1) these have considerable effects on insect digestive enzymes and as a result on their development; and 2) the transgenic plants expressing them are safe. In this study, the effects of NaCl, ethylenediamine tetraacetate disodium dehydrate (EDTA), Tris, sodium dodecyl sulfate (SDS) and an alpha-amylase inhibitor derived from wheat kernels (WAAI) on alpha-amylase activity of adults of the stripped bug Graphosoma lineatum (L.) during 60 minutes incubation was studied. Distilled water was considered as the control. The results showed that the effects of the inhibitor type on adult alpha-amylase activity was significant (P