Isolation and molecular diagnosis of Peste des petits ruminants (PPR) virus from contaminated areas in Iran

Iranian Journal of Veterinary Science and Technology

مقاله 4، دوره 9، شماره 1 - شماره پیاپی 16، آذر 2017، صفحه 17-21   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v9i1.56792

نویسنده

Roozbeh Fallahi^*

Razi Vaccine and Serum Research Institute

چکیده

Due to the numerous reports concerning the Peste des petits ruminants (PPR) in different regions of Iran, the isolation and genetic characterization of native isolates is very important. During 20132014, 168 samples were collected from whole blood, eye, nasal and oral swabs, and lymph nodes of sheep and goats with clinical signs in nine provinces with infected focal zones in Iran. Vero cell line and primary Lamb Kidney cells were inoculated with isolates and observed at least in 5 consecutive passages for cytopathic effects (CPE). The sheep samples from two provinces, created CPE in both kinds of cell cultures. Observation by electron microscopy and confirmation by RT-PCR was used to confirm PPR.

کلیدواژه‌ها

PPR؛ isolation؛ diagnosis؛ Iran

مراجع

Dhar P, Sreenivasa BP, Barrett T, Corteyn M, Sing RP, Bandyopadhyay SK, Recent epidemiology of peste de petits ruminants virus. Veterinary Microbiology. 2002; 88:153-159.

Kwiatek O, Minet C, Grillet C, Hurard C, Carlsson E, Karimov B, Albina E, Diallo A, Libeau G, Peste des petits ruminants (PPR) outbreak in Tajikistan. Journal of Comparative Pathology. 2007; 136;111–119.

Ozkul A, Akca Y, Feray Alkan F, Barrett T, Karaoglu T, Dagalp SB, Anderson J, Yesilbag K, Cokcaliskan C, Gencay A, Ibrahim Burgu I, Prevalence, distribution, and host range of Peste des petits ruminants virus, Turkey. Emerging Infectious Diseases. 2002; 8;708-712.

Zaher KS, Ahmed WM, Isolation and identification of field isolate of Peste des Petits Ruminants Virus in Egypt. Global Veterinaria. 2014; 12;667-672.

OIE, General disease information sheets. 2016.

6. Govindarajan R, Koteeswaran A, Venugopalan AT, Shyam G, Shaouna S, Shaila MS, Ramachandran S, Isolation of pestes des petits ruminants virus from an outbreak in Indian buffalo (Bubalus bubalis). Veterinary Record. 1997; 141:573-574.

Chauhan HC, Chandel BS, Kher HN Dadawala AI, Agrawal SM, Pesti des petits ruminants virus infection in animals. Veterinary World. 2009; 2:150-155.

Merck Veterinary Manual, Merck Sharp and Dohme Corp., A subsidiary of Merck & Co., Inc., Kenilworth, N.J, U.S.A. 2015.

Pal S Isore DP, Mukhopadhayay SK, Ganguly S, Das M, Seroprevalence and incidence of PPR among population of small ruminants in West Bengal, India. International Journal of Pharmaceutical Research and Bio-Science. 2014; 3:396-401.

Shaila MS, David S, Foryth MA, Diallo A, Goatley L, Kiching RP, Barret T, Geographical distribution and epidemiology of PPR viruses. Virus Research. 1996; 43:149-153.

Abdollahpour G, Raoofi A, Najafi J, Sasani F, Sakhaie E, Clinical and para clinical findings recent outbreaks of PPR in Iran. Journal of Veterinary Medicine. 2006; 53:14 -16.

Diallo A, LibeauG, Couacy-Hymann E, Barbron M, Recent developments in the diagnosis of rinderpest and peste des petits ruminants. Veterinary Microbiology. 1995; 44;307-317.

Zahur AB, Ullah A, Irshad H, Latif A, Ullah RW, Afzal M, Khan SA, Samo MH, Mahboob K, Isolation and characterization of lineage-IV Peste des Petits Ruminants (PPR) virus strains from Pakistan. International Journal of Innovation and Applied Studies. 2014; 8:185-194.

Anderson J, Barrett T, Scott GR, Manual on the Diagnosis of Rinderpest. Rome, Italy: Food and Agriculture Organization (FAO) of United Nations. 1996.

Nanda YP, Chatterjee A, Purohit AK, Diallo A, Innui K, Sharma RN, Libeau G, Thevasagayam JA, Bruning A, Kitching RP, Anderson J, Barrett T, Taylor WP, The isolation of peste des petits ruminants virus from Northern India. Veterinary Microbiology. 1996; 51:207-216.

Taylor WP, Al Busaidy S, Barrett T, The epidemiology of peste des petits ruminants in the Sultanate of Oman.Veterinary Microbiology. 1990; 22:341-352.

Forsyth M, Barrett T, Detection and differentiation of rinder pest and peste des petits ruminants viruses in diagnostic and experimental samples by polymerase chain reaction using P and F gene-specific primers. Virus Research. 1995; 39;151-163.

Couacy-Hymann E, Roger F, Hurard C, Guillou JP, Libeau G, Diallo A, Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. Journal of Virological Methods. 2002; 100:17–25.

Bahadar S, Anjum AA, Ahmad MD, Hanif A, Isolation and identification of peste des petits ruminants virus by cell culture and immunocapture enzyme linked immunosorbent assay. The Journal of Animal & Plant Sciences. 2009; 19:19-121.

Housawi FMT, Abu Elzein E M E, Mohamed G E , Gameel AA, Al-Afaleq AI, Hegazi A, Al-Bishr B, Emergence of Peste des Petits Ruminants in sheep and goats in eastern Saudi Arabia. Revue D’Elevage Et De Medecine Veterinaire Des Pays Tropicaux. 2004; 57;31-34.

Libeau G, Prehaud C, Lancelot R, Colas F, Guerre L, Bishop DHL, Diallo A, Development of a competitive ELISA for detecting antibodies to the Peste des petits ruminants virus using a recombinant nucleoprotein. Research in Veterinary Science. 1995; 58:50-55.

Balamurugan V, Sen A, Venkatesan G, Yadav V, Bhanot V, Riyesh T, Bhanuprakash V, Singh RK, Sequence and phylogenetic analyses of the structural genes of virulent isolates and vaccine strains of peste des petits ruminants virus from India. Transboundary and Emerging Diseases. 2010; 57:352–364.

Farooq U, Khan QM, Barret T, Molecular Based Diagnosis of Rinderpest and Peste Des Petits Ruminants Virus in Pakistan. International Journal of Agricultural Biology. 2008; 10:93-96.