Calibration of accelerated aging test as a vigor test to predict the seedling emergence of chickpea (Cicer arietinum L.) in field conditions

پژوهش‌های حبوبات ایران

Article 2, Volume 7, Issue 1, June 2016, Pages 9-24   

Document Type: Original Articles

DOI: 10.22067/ijpr.v7i1.33894

Authors

Parvin Bayat; Mokhtar Ghobadi^* ; Mohammad-Eghbal Ghobadi; Gholamreza Mohammadi

Razi University, Kermanshah

Abstract

Introduction
International Seed Testing Association (ISTA) defined seed vigour as "the sum of those properties of the seed which determine the level of activity and performance of the seed or seed lot during germination and seedling emergence". In any seed lot, losses of seed vigour results in the reduction of the ability of seeds to carry out all the physiological functions that allow them to perform. This process, called physiological ageing, starts before harvest and continues during harvest, processing and storage. Any given seed vigour test must be able to provide a more sensitive index of seed quality than the germination test and provide a consistent ranking of seed lots in terms of their potential performance. It must also be objective, rapid, simple and economically practical, reproducible and interpretable. Internationally, many vigour tests have been proposed such as standard germination test, cold test, electrical conductivity test, hiltner test, tetrazolium test, controlled deterioration test, accelerated aging test, etc. The accelerated aging test provides valuable information on storage and seedling field emergence po tentials. In the accelerated aging test, seeds are hydrated to a specific level when ex posed to relatively high temperature (40 to 45 oC) and humidity (around 100 % relative Humidity). Following this aging treatment, seeds are subjected to a germination test and higher vigor seed lots tolerate this aging condition better than lower vigor seed lots and produce a higher percentage of normal seedlings. In this study, chickpea seed lots were exposed to different temperatures and durations in order to calibration of accelerated aging test to predict the seedling emergence under field conditions.

Materials and Methods
This research was conducted as two laboratory and field tests at Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran, in 2012. In the laboratory test, accelerated aging treatments (temperature and duration) including 41, 43, and 45 oC and duration 0 (control), 48, 72, 96, 120, and 144 h were performed on 13 chickpea seed lots. Following this aging treatment, seeds were subjected to standard germination test and different traits (including final germination percentage, mean daily germination, daily germination speed, germination rate, germination index, seedling vigor index, and efficiency of seed storage usage) were evaluated. Theses 13 seed lots belonged to both kabloli (Hashem, Azad, ILC-482, Bivanij, Arman cultivars) and desi (Kaka and Pirooz cultivars) types. In the field tests, these 13 chickpea seed lots were planted as a randomized complete block design with four replications. In field test, different seedling traits (including final emergence percentage, mean time to emergence, mean daily emergence, daily emergence speed, emergence rate, and field emergency index) were evaluated. Finally, correlation of different traits related to seed vigor in the laboratory test was determined with percentage and rate of seedling emergence in the field.

Results and Discussion
Analysis of variance in the laboratory test showed that the effects of seed lots, accelerated aging treatments and their interaction were significant on all above mentioned traits except for efficiency of seed storage usage. Different levels of accelerated aging treatments had significant effects on germination characteristics, so that germination characteristics were decreased with increasing of temperature and duration in accelerated aging test. In the field test, chickpea seed lots were different significantly in most seedling traits. Means comparison of laboratory and field experiments showed that the new seed lots had higher germination and vigor characteristics than the old seed lots. Correlation analysis showed that, shoot dry weight in the accelerated aging test under 43 oC , 48 h and root length under 43 oC, 72 h had the highest correlation with seedling emergence percentage in the field test. Root length under 41 oC, 72 h and shoot dry weight under 43 oC, 72 h had the highest correlation with seedling emergence rate in the field test, too.

Conclusion
It seems that accelerated aging test is recommendable to predict the seedling emergence of chickpea in field conditions. The accelerated aging test under 41 oC, 72 h and 43 oC, 48 h had the highest correlation with both percentage and rate of seedling emergence in the field test.

Keywords

Deterioration; Germination; Seedling emergence; Seed lot; Seed vigor

References

1. Agrawal, R. 2003. Seed Technology .Pub .Co .PVT. LTD. New Delhi .India.

2. Akbari Gh.A., Ghasemi Pirbalouti M., Najaf Abadi Farahani M., and Shahverdi, M. 2004. Effect of harvesting time on soybean seed germination and vigor. Journal of Agriculture 6: 9-18. (In Persian with English Summary)

3. Association of Official Seed Analysts (AOSA). 1993. Seed Vigor Testing Handbook. No 45, 157pp.

4. Bailly, C., Benamar, A., Corbineau, F., and Come, D. 2000. Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Science Research 10: 35-42.

5. Basra, S.M.A., Ahmad, N., Khan, M.M., Iqbal N., and Cheema, M.A. 2003. Assessment of cotton seed deterioration during accelerated aging. Seed Science and Technology 31: 531-540.

6. Benbest. 2007. Mechanism of aging. www.benbest.com/ lifeext/aging.html.

7. Bingham, I., Harris, J.A., and MacDonald, L. 1994. A comparative study of radicle and coleoptile extension in maize seedlings from aged and un-aged seed. Seed Science and Technology 22: 127-139.

8. Bishnoi, U.R., and Santos, M.M. 1996. Evaluation of seed of three mungbean cultivars for storability, quality and field performance. Seed Science and Technology 24: 237-243.

9. Chhetri, D.R., Rai, A.S., and Bhattacharjee, A. 1993. Chemical Manipulation of seed longevity of four crop species in an unfavorable storage environment. Seed Science and Technology 21: 31-44.

10. Damavandi, A., Latifi, N., and Dashtban, A.R. 2007. Evaluation of Seed vigour tests and its field efficiency in forage sorghum (Sorghum bicolor L.). Journal of Agricultural Sciences and Natural Resources 14(5): 17-24. (In Persian with English Summary).

11. De Figueiredeo, E., Albuquerque, M.C., and Carvalho, N.M. 2003. Effect of the type of environmental stress on the emergence of sunflowers (Helianthus annua L.), soybean (Glycine max L.) and maize (Zea mays L.) seed with different levels of vigor. Seed Science and Technology 31: 531-540.

12. Dehghanshoar, M., Hamidi, A., and Mobasser, S. 2005. Handbook of Vigour Test Methods. Agricultural Education Press. 193 pp. (In Persian).

13. Dell Aquila, A. 1994. Wheat seed ageing and embryo protein degradation. Seed Science Research 4: 239-298.

14. Durrant, M.J., Mash, S.J., and Jaggard, K.W. 1993. Effect of seed advancement and sowing date on establishment, bolting and yield of sugar beet. Journal of Agricultural Science 121: 333-341.

15. Ellis, R.H., and Roberts, E.H. 1981. The quantification of aging and survival in orthodox seeds. Seed Science and Technology 9: 377-409.

16. Ellis, R.H. 1992. Seed and seedling vigour in relation to crop growth and yield. Plant Growth Regulation 11: 249-255.

17. Gharineh, M.H., Bakhshandeh, A.M., and Ghassemi-Golezani, K. 2004. Effects of viability and vigour of seed on establishment and grain yield of wheat cultivars in field conditions. Seed and Plant Improvement Journal 20(3): 383-400. (In Persian with English Summary).

18. Ghassemi-Golezani, K., Dalil, B., Moghaddam, M., and Raey, Y. 2011. Effects of accelerated aging on soybean seed germination indexes at laboratory conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39(2): 160-163.

19. Goel, A., and Sheoran, I.S. 2003. Lipid peroxidation and peroxide- scavenging enzyme in cotton seeds under natural ageing. Biology Plant 46: 429-434.

20. Gusta, L.V., Johnson, E.V., Nesbitt, N.T., and Klikland, K.J. 2003. Effect of seeding date on canola seed quality and seed vigour. Canadian Journal of Plant Science 84: 463-471.

21. Hampton, J.G., and Tekrony, D.M. 1995. Handbook of Vigour Test Methods (3rd Ed.). International Seed Testing Association (ISTA). Zurich, Switzerland .117p.

22. Hampton, J.G. 2003. Methods of viability and vigour testing: a critical and appraisal. In: A.S. Basra, (Ed.). Seed Quality, Basic Mechanisms and Agricultural Implications. CBS Publishers and Distributers, New Delhi, India. p. 81-118.

23. Heydecker, W. 1977. Stress and seed germination: an agronomic view. In: A. Khan (Ed.). The Physiology and Biochemistry of Seed Dormancy and Germination. Elsevier/North Holland and Biomedical Press, Amsterdam. p. 237-282.

24. Hunter, E.A., Glasbey, C.A., and Naylor, R.E.L. 1984. The analysis of data from germination tests. Journal of Agricultural Science Cambridge 102: 207-213.

25. International Seed Testing Association (ISTA). 2012. International Rules for Seed Testing, 2012 edn. Bassersdorf, Switzerland: The International Seed testing Association (ISTA).

26. International Seed Testing Association (ISTA). 2003. Handbook for Seedling Evaluation (3rd. Ed.). International Seed Testing Association. Zurich, Switzerland. 223pp.

27. International Seed Testing Association (ISTA). 1996. International Rules for Seed Testing. Supplement to Seed Science and Technology, 24, Supplement.

28. Khalaj, H. 2006. Investigation of different hardness of drought stress during growth and development period on quality characteristics and vigourity of winter rapeseed cultivars. MSc. Thesis. University Tehran. 148 pp.

29. Kapoor, N., Arya, A., Siddiqui, M.A., Amir, A., and Kumar, H. 2010. Seed deterioration in chickpea (Cicer arietinum L.) under accelerated ageing. Asian Journal of Plant Sciences 9(3): 158-162.

30. Krishnan, P., Nagarajan, S., Dadlani, M., and Moharir, A.V. 2003. Characterization of wheat (Triticum aestivum L.) and soybean (Glycine max L.) seeds under accelerated ageing conditions by proton nuclear magnetic spectroscopy. Seed Science and Technology 31: 541-550.

31. Lehner, A., Mamadou, N., Poels, P., Come, D., Bailly, C., and Corbineau, F. 2008. Change in soluble carbohydrates, lipid peroxidation and antioxidant enzyme activities in the embryo during aging in wheat grains. Journal of Cereal Science 47(3): 555-565.

32. Maguire, J.D. 1962. Speed of germination, aid in selection and evaluation for seedling emergence and vigour. Crop Science 2: 176-177.

33. Makkawi, M., El Balla., M. Bishaw, Z., and Van Gastel, A.J.G. 1999. The relationship between seed vigor tests and field emergence in lentil. Seed Science and Technology 27: 657-668.

34. Matthews, S., and Khajeh Hosseini, M. 2006. Mean germination time as an indicator of emergence performance in soil of seed lots of maize (Zea mays L.). Seed Science and Technology 34: 339-347.

35. McDonald, C.M., Floyd, C.D., and Waniska, R.D. 2004. Effect of accelerated aging on maize, sorghun and sorghum. Journal of cereal Science 39: 351- 301.

36. McDonald, M.B. 1999. Seed deterioration: physiology, repair and assessment. Seed Science and Technology 27: 177-237.

37. Mohammadi, H., Soltani, A., Sadeghipour, H.R., and Zeinali, E. 2011. Effects of seed aging on subsequent seed reserve utilization and seedling growth in soybean. International Journal of Plant Production 5(1): 65-70.

38. Moshatati, A., and Gharineh, M.H. 2012. Effect of grain weight on germination and seed vigor of wheat. International Journal of Agriculture and Crop Sciences 4(8): 458-460.

39. Mosavi Nik, S.M., Gholami tilebeni, H., Kord firouz jae, Gh., Sadeghi, M., and Sedighi, E. 2011. Free fatty acid and electrical conductivity changes in cotton seed (Gossypium hirsutum L.) Under seed deteriorating conditions. International Journal of Agriculture Science 1(2): 62-66.

40. Murthy, U.M.N., Kumar, P.P., and Sun, W.Q. 2003. Mechanisms of seed ageing under different storage conditions for Vigna radiate Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. Journal of Experimental Botany 54: 1057-1067.

41. Pandey, P.K., Goyal, R.D., Parakash, V.R., Katiyar, P., and Singh. 1990. Association between laboratory vigour tests and field emergence in cucurbits. Seed Research 18: 43-40.

42. Rajjou, L., and Debeaujon, I. 2008. Seed longevity: survival and maintenance of high germination ability of dry seeds. Comptes Rendus Biologies 331: 796-805.

43. Rama, C., kumara, P., singh, O., and Sadana, R.k. 1999. Relationship between seed vigoar tests and field emergence in chickpea. Seed Science and Technology 17: 169-173.

44. Rebetzke, G.S., and Richards, R.A. 1999. Genetic improvement of early vigour in wheat. Australian Journal Arabic Research 50: 291-301.

45. Roberts, E.H., and Osei-Bonsu, K. 1988. Seed and seedling vigour. R.J. Summer Field. (Ed.) word crops: cool season food legumes London, p. 897-970.

46. Rozrokh, M., Ghasemi Golozani, K., and Javanshir, A. 2002. Relation between seed vigour and field performance in chickpea (Cicer arietinum L.). Journal of Agriculture Research Seed and Plant Improvement Institute 18(2): 156-162. (In Persian with English Summary).

47. Saha, R.R., and Sultana, W. 2008. Influence of seed ageing on growth and yield of soybean. Bangladesh Journal Botany 37: 6-21.

48. Salvucci, M.E., and Crafts Brandner, S.J. 2004. Inhibition of photosynthesis by heat stress: the activation state of Rubisco as a limiting factor in photosynthesis. Physiologia Plantarum 120: 179-186.

49. Scott, S.J., Jones, R.A., and Williams, W.A. 1984. Review of data analysis methods for seed germination. Crop Science 24: 1192-1199.

50. Soltani, E., Ghaderi, A., and Memar, H. 2008. The effect of priming on germination components and seeding growth of cotton seeds under drought. Journal Agricultural Science Natural Research 14(5): 9-16. (In Persian with English Summary).

51. Soltani, A., Kamkar, B., Galeshi, S., and Akram Ghaderi, F. 2007. Effect of seed storage on resource depletion and heteroterophic growth of wheat seedling. Iranian Journal of Agricultural Science 15: 229-259. (In Persian with English Summary).

52. Soltani, A., Gholipoor, M., and Zeinali, E. 2006. Seed reserve utilization and seedling of wheat as affected by drought and salinity. Environmental Experimental Botany 55: 195-200.

53. Steiner, J.J. 1990. Seedling rate of development index: indicator of vigor and seedling growth response. Crop Science 30: 1264-1271.

54. Sung, J.M. 1996. Lipid peroxidation and peroxide-scavenging in soybean seeds during aging. Physioligia Plantarum 97: 85-89.

55. Sung, J.M., and Jeng, T.L. 1994. Lipid peroxidation and peroxide-scavenging enzymes associated with accelerated aging of peanut seed. Physioligia Plantarum 91: 51-55.

56. TeKrony, D.M., and Egli, D.B. 1991. Relationship of seed vigor to crop yield: A Review. Crop Science: 31: 816-822.

57. Tilebeni, G.H., and Golpayegani, A. 2011. Effect of seed ageing on physiological and biochemical changes in rice seed (Oryza sativa L.). International Journal of AgriScience 1(3): 138-143.

58. Wen, S.H.T., and Kung-Cheheng, M.E. 1990. Relationship between seed health, seed vigour and the performance of sorghum in the field. Seed Science and Technology 18: 713-719.