Genetic diversity and relationships between some agronomic traits of chickpea genotypes (Cicer arietinum L.) under non-stress and terminal drought stress conditions

پژوهش‌های حبوبات ایران

Article 5, Volume 8, Issue 1, June 2017, Pages 83-96   

Document Type: Original Articles

DOI: 10.22067/ijpr.v8i1.48717

Authors

Maryam Rezaeinia^* ; Mohammad Reza Bihamta; Seyed Ali Peyghambari; Alireza Abbasi; Fatemeh Gharajedaghi

University of Tehran

Abstract

Introduction
Legumes are one of the most important sources of protein in the diet of many people in developing countries and are the second largest source of human food. Chickpea (Cicer arietinum L.) ranks 2nd in cultivated area of legumes in the world. Low chickpea performance in Iran with respect to environmental conditions and inaccessibility to adequate water and appropriate genotype and little breeding works has been done on it. Since the main components of genetic and environmental factors that determine the yield and quality of plants, the main purpose of the selection of breeding indices, should focus more on the effects of genetic factors. Genetic diversity in chick pea collection can be used in breeding program for selection of genotype with desirable agronomic traits. Studying relationships between agronomic traits would assist breeders to identify the effective traits and use proper selection intensity in their breeding programs.

Material & Methods
In order to evaluate the genetic diversity and identify relationships between yield with other morphological traits, in chickpeas genotype, 28 Kabuli genotypes with two controls i.e. Jam and Kourosh, under non-stress and terminal drought stress condition, an experiment was carried out in a complete block design with three replications on research farm of University of Tehran in 2013.

Results & Discussion
For most of the traits, results showed that there were significant differences among genotypes which revealed genetic variation among them. The results of phenotypic correlation under non-stress condition showed that seed yield per plant had significant and positive correlation with seed and pod weight per plant, number of seeds per plant and 100 seed weight. Results under drought stress condition showed that seed yield per plant with seed and pod weight and number of seeds per plant had significant and positive correlation at 1% probability level. According to the results of phenotypic correlations, stepwise regression, path analysis in both conditions (non-stress and with terminal stress), it could be concluded that, the traits such as seed and pod weight per plant, 100-seed weight, and number of seeds per plant were most important and effective traits affecting yield and considering that among the genotypes. Since most variation was observed among genotypes for these traits, therefore selecting and breeding for them could be ideal for improving yield. Based on factor analysis under the non-stress condition, the three factors were selected that explained 69.3 percent of the total variations. The first, second, and third factors were explained 30.32, 26.78 and 12.28 percent of the variations, respectively. The second factor that included five traits (seed and pod weights per plant, number of seeds per plant, seed yield per plant and number of seed per pod) was introduced as the components of yield. Under the terminal drought stress condition, four factors selected that totally were explained 89.6 percent of the variations. The first, second, third and fourth factors were explained as 44.86, 23.43, 11.82 and 9.56 percent of the variations, respectively. In these conditions also, the second factor that included four traits (seed and pod weights per plant, number of seeds per plant and seed yield per plant) was introduced as components of yield. Therefore, these two factors used to identify genotypes with high yield and yield components, in non-stress condition the genotypes 22,474,239,370 and control genotype Jam (No. 998), and in stress condition the genotypes 508,236 with two control genotypes Jam (No. 998) and Kourosh (No, 999) were selected as high yield and component yield genotypes. According to the results of cluster analysis based on the square Euclidian distance and Ward method, under non-drought stress condition the genotypes classified in 3 clusters. The genotypes of second cluster had high yield comparing with the other clusters and total genotypes average. According to the results of cluster analysis under drought stress condition, the genotypes classified in 4 clusters that the genotypes of third cluster, in seed and pod weights per plant, number of seeds per plant, seed yield per plant and number of seed per pod traits had the highest average comparing with other clusters and total genotypes average. According to the result of cluster analysis, the genotypes in third cluster with two control genotypes (Jam and Kourosh) could be use for produce hybrids and getting yield hybrids.

Conclusion
According to the results, the studied chickpea germplasm, are valuable resources, in addition to possess new traits provide high diversity for breeders to improve of the new varieties.

Keywords

Cluster analysis; Factor analysis; morphological traits; Path-analysis

References

1. Acikgoz, N., and Acikgoz, N. 1994. Path analysis for evaluation of characters affecting seed yield in chickpeas at different sowing time. Crop Science Congress 2: 121-125.

2. Acquaah, G., Adams, M.W., and Kelly, J.D. 1991. Identification of effective indication of erect plant arquitecture in dry bean ideotype. Crop Science 31: 261-264.

3. Ali Pour Yamchi, H., Bihamta, M.R., Peighambari, S.A., Naghavi, M., and Shafiee Khorshidi, M. 2011. Evaluation of genetic diversity and classification of Kabuli chickpea genotypes in late season drought stress .Journal of Crop Breeding 3(7): 53-70. (In Persian with English Summary).

4. Ali, Q., Ahsan, M., Khan, N.H., Ali, F., Elahi, M., and Elahi, F. 2012. Genetic analysis for various quantitative traits of chickpea (Cicer arietinum L.). International Journal for Agro Veterinary and Medical Sciences 6(1): 51-57.

5. Bernier, J., Altin, G.N., Serraj, R., Kumar, A., and Spaner, D. 2007. Review: Breeding upland rice for drought resistance. International Rice Research Institute. 33P.

6. Broughton, W.J.G., Hernandez, M., Blair, S., Beebe, P., and Vanderleyden, J. 2003. Beans (Phaseolus spp) model food legume. Plant Soil 252: 55-128.

7. Chaghamirza, K., and Farshadfar, E. 2005. Study of relationships between yield and yield components in chickpea. Abstracts of the 9th Iranian Congress of Crop Science and Plant Breeding, College of Agriculture, Unicersity of Tehran, Karaj, Iran. (In Persian).

8. Cheghamirza, S. 2007. Evaluation of Genetic Diversity in Chickpea using Agronomic Traits. MSc. Thesis, Faculty of Agriculture, Islamic Azad University of Kermanshah, Kermanshah, Iran (In Persian).

9. Ciftci, V., Togay, N., Togay, Y., and Dogan, Y. 2004. Determining relationships among yield and some yield components using path coefficient analysis in chickpea (Cicer arietinum L.). Asian Journal of Plant Sciences 3(5): 632-635.

10. Ebrahimi, M., Bihamta, M.R., Hossein zadeh, A.H., Khiyalparast, F., and Golpashi, M. 2010. Evaluation of reaction yield and yield components of white bean genotypes under water stress. Iranian Journal of Field Crops Research 8: 347-358. (In Persian).

11. FAO. 2008. Food Outlook, Global Market Analysis. http://www.fao.Food outlook.com.

12. Farshadfar, A., Zamani, M.R., Motallebi, M., and Emam Jomae, A. 2001. Selection for drought resistance in chickpea lines. Iranian Journal of Agricultural Sciences 32 (4): 65-77. (In Persian).

13. Fazeli, F., and Cheghamirza, K. 2011. Genetic variation in Iranian chickpea (Cicer arietinum L. Kabuli type) based on agronomic traits and RAPD marker. Seed and Plant Improvement Journal 1-27(4): 555-579. (In Persian).

14. Ganjeali, A., Porsa, H., and Bagheri, A. 2011. Response of yield and morphophysiological characteristics of earliness chickpea genotypes (Cicer arietinum L.) under drought stress. Iranian Journal of Pulses Research 2(1): 65-80. (In Persian with English Summary).

15. ICARDA. 1997. Food Legume Improvement Program: Annual Report 1996. Aleppo. Syria: ICARDA.

16. Kanouni, H., and Malhotra, R.S. 2003. Genetic variation and relationships between traits in chickpea (Cicer arietinum L.) lines under dryland conditions. Iranian Journal of Field Crop Science 5(3):185-193. (In Persian with English Summary).

17. Kayan, N., and Adak, M.S. 2012. Associations of some characters with grain yield in Chickpea (Cicer arietinum L.). Pakistan Journal Botany 44(1): 267-272.

18. Malik, S.R., Bakhsh, A., Asif, M.A., Iqbal, U., and Iqbal, S.M. 2010. Assessment of genetic variability and interrelationship among some agronomic traits in chickpea. International Journal of Agriculture and Biology 12(1): 81-85.

19. Mardi, M., Taleei, A.R., and Omidi, M. 2003. Study of genetic diversity and identification of yield components in desi chickpea. Iranian Journal of Agricultural Science 34(2): 345-351. (In Persian).

20. Meena, H.P., Kumar, J., Upadhyaya, H.D., Bharadwaj, C., Chauhan, S.K., Verma, A.K., and Rizvi, A.H. 2010. Chickpea mini core germplasm collection as rich sources of diversity for crop improvement. Journal of SAT Agricultural Research 8: 1-5.

21. Nielsen, D.C. 2001. Production function for chickpea, field pea, and lentil in the central great plains. Agronomy Journal 93: 563-569.

22. Ozdemir, S. 1996. Path coefficient analysis for yield and its components in chickpea. International Chickpea and Pigeonpea Newsletter 3: 9-21.

23. Rezaeyan Zadeh, E., Parsa, M., Ganjali, A., and Nezami, A. 2011. Responses of yield and yield components of chickpea cultivars (Cicer arietinum L.) to supplemental irrigation in different phenology stages. Journal of Water and Soil 25(5): 1080-1095. ( In Persian with English Summary).

24. Sabokdast, M., and Khyalparast, F. 2008. A study of relationship between grain yield and yield components in Common bean cultivars (Phaseolus vulgaris L.). Journal of Science and Technology of Agriculture and Natural of Resource 11(42) : 123-134. (in Persian).

25. Sharma, P.P., and Maloo S.R. 1988. Correlation and path coefficient analysis in Bengal gram (Cicer arietinum L.). Journal of Maharashtra Agriculture University 75(3-4): 95-98.

26. Siddique, K.H.M., Loss S.P., and Thomsons, B.D. 2003. Cool seasons grain legume in dry land Mediterranean environment of western Australia: Significance of early flowering. pp: 151-163. In: N.P. Saxena (Ed.). Management of Agriculture Drought “Agronomic and Genetic Options”. Science Publishers Inc, NH, USA.

27. Singh, K.B., and Saxena, M.C.1999. Studies on drought tolerance. Annual Report, ICARDA, Aleppo, Syria. pp: 108-113.

28. Singh, K.B., Bejiga, G., and Malhotra, R.S. 1990. Associations of some characters with seed yield in chickpea collection. Euphytica 49: 83-88.

29. Takeda, S., and Matsuoka, M. 2008. Genetic approaches to crop improvement: responding to environmental and population change. Nature 9: 444-457.

30. Toker, G., and Cagirgan, M.I. 2004. The use of phenotypic correlations and factor analysis in determining characters for grain yield selection in chickpea (Cicer arietinum L.). Hereditas 140: 226-228.

31. Uddin, M.J., Hamid, M.A., Rahman, A.R.M.S., and Newaz, M.A. 1990. Variability, correlation and path analysis in chickpea (Cicer arietinum L.) in Bangladesh. Bangladesh Journal, Plant Breeding and Genetics 3: 51-55.

32. Viakumar, C., Salmath, P.M., Goud, J.V., and Parameshw, R. 1991. Genetic variability and genotype environment interaction in Chickpea. Journal of Maharashtra Agriculture University 16(1): 37-39.

33. Yousefi, B., Kazemi Arbat, H., RahimZadeh Khoyi, F., and Moghadam, M. 1997. Study for some agronomic traits in chickpea cultivars under two irrigation regimes and path analysis of traits under study. Journal of Agricultural Sciences 147-162. (In Persian with English Summary).

34. Yucel, D.O., Anlarsal, A.E., and Yucel, C. 2006. Genetic variability, correlation andpath analysis of yield, and yield components in chickpea (Cicer arietinum L.). Turkish Journal of Agriculture and Forestry 30: 183-188.

35. Zali, H., Farshadfar, E., and Sabaghpour, S.H. 2011. Genetic variability and interrelationships among agronomic traits in chickpea (Cicer arietinum L.) genotypes. Crop Breeding Journal 1(2): 127-132.