Brief report Detection of Plum Pox Virus (PPV) from Stone Fruits in Khorasan Razavi Province, Iran

پژوهش های حفاظت گیاهان ایران

Article 16, Volume 27, Issue 1 - Serial Number 22, March 2013, Pages 132-135   

Document Type: Research Article

DOI: 10.22067/jpp.v27i1.22172

Authors

B. Jafarpour^* ; Mohammad Ali Sabokkhiz

Abstract

Plum pox virus (PPV), the causal agent of Sharka disease, belongs to the genus Potyvirus in the family Potyviridae and has been reported from some regions of Iran. Surveys were conducted to determine the incidence of the virus in Khorasan Razavi province in 2010 and 2011 and 553 samples of stone fruit trees were collected and tested by DAS-ELISA. In this method 12 samples were infected with PPV. RT-PCR with specific primers amplified 467bp band in 3 samples. DNA fragment of Kadkan region was sequenced after purification from gel. The determined sequence of IRN-KDN isolate were compared with previously reported 29 PPV isolates, using ClustalW2. In phylogenetic analyses based on ClustalW multiple alignment and MEGA 5.1, IRN KDN displayed the highest (98% and 96%) identity with two Japanese isolates and the lowest (95% and 90%) identity with isolate of Slovakia at nucleotide and amino acid sequence levels, respectively.

Keywords

Plum pox virus (PPV); DAS-ELISA; RT-PCR; Khorasan Razavi; Iran