Study of polymorphism in Mazandaran and Esfahan native chicken population using microsatellite markers

پژوهشهای علوم دامی ایران

Article 1, Volume 6, Issue 2 - Serial Number 18, June 2014   

Document Type: Genetics & breeding

DOI: 10.22067/ijasr.v6i2.24013

Authors

Saeid Esmaeilkhanian^{* 1}; Saeed Ansari Mahyari²; Ardeshir Nejati Javaremi; Hossein Banabazi³; Majid Sadeghian; Maryam Tatari

¹Isfahan Agricultural and Natural Resources Research Center

²Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran

³Department of Biotechnology, Animal Science Research Institute of IRAN (ASRI), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran

Abstract

In order to studying of genetic variation in Mazandaran and Esfahan native chickens, twenty microsatellite markers were evaluated. These microsatellite markers were MCW0014, MCW0081, MCW0183, MCW0067, MCW0104, MCW0123, MCW0330, MCW0165, MCW0069, MCW0020, MCW0222, LEI0094, MCW0295, MCW0034, MCW0216, ADL0268, ADL0112, ADL0278 and lEI0166. Blood samples of 90 and 150 native chickens of Mazandaran and Esfahan were randomly taken respectively. Genomic DNAs were isolated through optimized and modified salting-out procedure. The number of alleles varied from 1 to 6. In Mazandaran population one locus (MCW0216) and in Esfahan population three loci (MCW0216, MCW67 and MCW222) were monomorphic. The other loci were showed appropriate polymorphism. All the loci except MCW222 and MCW165 in Mazandaran population showed deviations from Hardy-Weinberg equilibrium (p

Keywords

locus; Polymorphism; effective allele; Heterozygosity