Polymorphism in exon2 of BMP15 gene in Iranian sangsari sheep

پژوهشهای علوم دامی ایران

Article 9, Volume 6, Issue 4 - Serial Number 20, December 2014, Pages 381-387   

Document Type: Genetics & breeding

DOI: 10.22067/ijasr.v6i4.27504

Authors

zana pirkhezranian^{* 1}; Arezoo Mohammadhashemi¹; Mojtaba Tahmoorespur¹; Nasrollah Pirany²

¹Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

²Department of Animal Science, Faculty of Agriculture, Shahrekord University, Iran.

Abstract

Fertility rate is an economically important trait in sheep, which is influenced by genetic and environment. So far, three genes have been identified that affects this trait, one of them would be the BMP family, the most famous one is BMP15. Different mutations in the BMP15 gene, increases reproductive performance and growth rate in sheep. The aim of this study was to investigate the genetic and phylogenetic of BMP15 gene sequence in Iranian Sangsari sheep. For this purpose, the blood samples from 20 animal of Damghan station were collected. After DNA extracting, a segment of 222 bp of exon 2 of BMP15 gene was amplified using polymerase chain reaction. Then, all of the PCR products were sequenced. The results showed existence of four haplotypes and three significant mutations of the gene that which one of them was seen for first. In order to determine the genetic distance of Sansari sheep with other animals especially sheep breeds about 103 sequences were taken from Genebank, Then, phylogenetic trees were drawn. Genetic distances and nucleotide differences were calculated. The results showed that goat, cattle and buffalo have minimum genetic distance and monkey, human and mouse have maximum distance with Sangsari sheep and native Hindi and Kashmiri sheep have not any differences with Iranian Sangsari sheep.

Keywords

Sangsari Sheep; BMP15 gene; phylogenetic tree; Genetic distance

References

1- سلیمانی، ب.، ق.ا. رحیمی میانجی.، و ب. چهار آیین. 1390. بررسی اثر پلی مورفیسم در اگزون 2 ژن BMP15 روی دوقلو زایی و صفات وزنی در گوسفند سنجابی. مجله زیست شناسی ایران. جلد 24، شماره 4. 493-487.

2- نصیری، م. ر.، و م. مهدوی. 1390. تجزیه و تحلیل ژنتیکی و فیلوژنتیکی ناحیه سیتوکرم b در جبیر ایران. مجله بیوتکنولوژی کشاورزی. دوره 3، شماره 1. 104- 91.

3-ولی زاده، ر. 1390. پرورش گوسفند و بز. انتشارات دانشگاه فردوسی مشهد. صفحه 569.

4- Choi, D., S. S. Hwang., E. Y. Lee., C. E. Park., B. K. Yoon., J. H. Lee., and D. S. Bae. 2003. Recombinant FSH and pregnancy-associated plasma protein. Eur J Obstet Gynecol Reprod Biol. 109:171–176.

5- Davis, G. H. 2005. Major genes affecting ovulation rate in sheep. Genet. Sel. Evol. 37 (Suppl. 1) S11–S23.

6- Dube, J. L., P. Wang., J. Elvin., K. M. Lyons., A. J. Celeste., and M. M. Matzuk. 1998. The Bone Morphogenetic Protein15 Gene Is X-Linked and Expressed in Oocytes. Mol. Endocrinol. 12: 1809–1817.

7- Galloway, S.M., S.M. Gregan., T. Wilson., P. McNattyK., J.L. Jungel., O. Ritvos., and G.H. Daivis. 2002. BMP15 mutations and ovarian function. Mol.Cell Endocrinol. 191:15-18.

8- Galloway, S.M., K.P. McNatty., L.M. Cambridge., M.P.E. Laitinen., J.L. Juengel., T.S. Jokiranta., and et al. 2002. Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nat Genet. 25:279–283.

9- Ghaffari, M., A. Nejati-Javaremi., and G. Rahimi. 2009. Detection of polymorphism in BMPR-IB Gene associated with Twinning in Shal sheep using PCR-RFLP method. Int J Agric Biol. 11:97-99.

10- Hanrahan, J.P., S.M. Gregan., P. Mulsant., M. Mullen., G.H. Davis., R. Powell., and S.M. Galloway. 2004. Mutations in the genes for Oocyte-derivedgrowth factors GDF9 and BMP15 are associatedwith bothincreased ovulation rate and sterilityin Cambridge and Belclare sheep (Ovis Aries). Biol Reprod. 70: 900-909

11- Hussein, T.S., D.A. Froiland., F. Amato., J.G. Thompson., and R.B. Gilchrist. 2005. Oocytes prevent cumulus cell apoptosis by maintaining a morphogenic paracrine gradient of bone morphogenetic proteins. J Cell Sci 118:5257–5268.

12-Juengel, J.L., N.L. Hudson., D.A. Heath., P. Smith., K.L. Reader., S.B. Lawrence., and et al. 2002. Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep. Biol Reprod. 67:1777–1789.

13- Laitinen, M., Vuojolainen, K., Jaatinen, R., Ketola, I., Aaltonen, J., Lehtonen, E., Heikinheimo, M., and Ritvos, O. 1998. A novel growth differentiation factor-9 (GDF-9) related factor is co-expressed with GDF-9 in mouse oocytes during folliculogenesis. Mech. Dev. 78, 135–140.

14- McNatty, K.P., S.M. Galloway., T. Wilson., P. Smith., N.L. Hudson., A. O’Connell., and et al. 2005. Physiological effects of major genes affecting ovulation rate in sheep. Genet Sel Evol. 37: S25–S38.

15- Nejati-Javaremi, M., G. Rahimi., and A. Amiri. 2007. Detection of polymorphism in FecXL gene associated with Twinning in Lori-Bakhtiari sheep breed using PCR-SSCP. The 5th National Biotechnology Congress of Iran. Tehran, Iran. P. 520

16- Otsuka, F., K.J. McTavish., and S. Shimasaki. 2011. Integral role of GDF-9 and BMP-15 in ovarian function. Mol Reprod Dev. 78:9–21.

17- Otsuka, F., S. Yamamoto., G.F. Erickson., and S. Shimasaki. 2001. Bone morphogenetic protein-15 inhibits follicle-stimulating hormone (FSH) action by suppressing FSH receptor expression. J Biol Chem. 276:11387–11392.

18- Shabir, M., T.A.S. Ganai., S.S. Misra., and et al. 2013. Polymorphism study of growth differention factor 9B (GDF9) gene and its association with reproductive traits in sheep. Gene 515:432-438

19- Sugiura, K., Y.Q. Su., F.J. Diaz., S.A. Pangas., S. Sharma., K. Wigglesworth., M.J. O'Brien., M.M. Matzuk., S. Shimasaki., and J.J. Eppig. 2007. Oocyte-derived BMP15 and FGFs cooperate to promote glycolysis in cumulus cells. Development. 134:2593–2603.

20- Yoshino, O., H.E. McMahon., S. Sharma., and S. Shimasaki. 2006. A unique preovulatory expression pattern plays a key role in the physiological functions of BMP-15 in the mouse. Proc Natl Acad Sci USA. 103:10678–10683.

21- Zare, Y., A. Nejati-Javaremi., and G. Rahimi. 2007. Detection of polymorphisms in two point of gene associated with Twinning (BMP15) in Shal sheep. The 5th National Biotechnology Congress of Iran. Tehran, Iran. P. 483