Effects of mycorrhiza inoculation and different irrigation levels on yield, yield components and essential oil contents of fennel (Foeniculum vulgare Mill.) and ajwain (Trachyspermum ammi L.)

بوم شناسی کشاورزی

Article 2, Volume 7, Issue 1 - Serial Number 23, March 2015, Pages 20-37   

Document Type: Scientific - Research

DOI: 10.22067/jag.v7i1.48191

Authors

Alireza Koocheki^{* 1}; Javad Shabahang¹; Surur Khorramel¹; Farzad Najafi²

¹Department of Agronomy, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

²Medicinal Plants and Drugs Research Institue, Shahid Beheshti University, Tehran, Iran

Abstract

Introduction
Fertilizers are the key components which provide plant nutrients' needs in recent years (Omid Jangir & Sing, 1996; Kapoor et al., 2007). In many cases, using chemical fertilizers has different negative environmental effects such as soil, water and air pollution, which increase environmental hazardous and production costs (Jangir & Sing, 1996; Kapoor et al., 2007). Biological activities are markedly enhanced by microbial interactions in the rhizosphere of plants (Kapoor et al., 2007).
Many investigators have successfully used mycorrhiza to increase the availability of immobilized phosphate and thus minimize the use of mineral fertilizers. Arbuscular Mycorrhizal Fungi (AMF) can better enable a plant to withstand environmental stresses such as drought and salinity. AMF interacts with pathogens and other rhizosphere inhabitants which affect plant health and nutrition. More importantly, mycorrhizal fungi are capable of dissolving weakly soluble soil minerals, especially phosphate, by releasing acids or increasing CO2 partial pressure (Gupta et al., 2002; Gosling et al., 2006; Kapoor et al., 2007). Therefore, they have the ability to enhance host plant uptake of relatively immobile nutrients particularly P, S and Zn.
Limited water supply is also another major environmental constraint in the productivity of crop and medicinal plants. Moisture deficiency induces various physiological and metabolic responses such as stomatal closure, decline in growth rate and photosynthesis (Flexas and Medrano, 2002). The results of Baher et al. (2002) showed that greater soil water stress decreased plant height and total fresh and dry weight of Satureja hortensis.

Materials and Methods
In order to study the effects of mycorrhiza inoculation and different irrigation levels on the growth, quantitative and qualitative yield of fennel (Foeniculum vulgare Mill.) and ajwain (Trachyspermum ammi L.), a field experiment was conducted as factorial based on randomized complete block design with three replications at the Agricultural Research Station, the Ferdowsi University of Mashhad, Iran during two growing seasons of 2009-2010 and 2010-2011. Mycorrhiza inoculation (with and without inoculation) and irrigation levels (1000, 2000 and 3000 m3.ha-1) were allocated to the first and the second factors, respectively. Several criteria such as yield components (including branch numbers per plant, umbel number per branch, umbellet number per umbel, seed number per umbellet and 1000-seed weight), biological yield, seed yield, harvest index, essential oil content and essential oil yield of fennel and ajwain were measured.

Results and Discussion
Results indicated that the simple effects of mycorrhiza inoculation and irrigation levels on the biological and seed yields, harvest index (HI), yield components, essential oil content and essential oil yield of fennel and ajwain were significant (p≤0.01). The maximum biological yield of fennel (5.3 g.m-2) and ajwain (4.3 g.m-2) were observed in mycorrhiza inoculation. Mycorrhiza inoculation enhanced seed yield of fennel and ajwain up to 46% and 97% compared with control, respectively. The highest essential oil content of fennel (4.2%) and ajwain (3.0%) were obtained in mycorrhiza inoculation. The highest and the lowest seed yield of fennel and ajwain were observed in 3000 m3.ha-1 (1.6 and 0.9 g.m-2) and 1000 m3.ha-1 (1.4 and 0.7 g.m-2) irrigation levels, respectively. The maximum essential oil content of fennel and ajwain were obtained in 3000 m3.ha-1 (4.0% and 3.4%) and the minimum were for 1000 m3.ha-1 (3.2% and 2.9%). Interaction effects among mycorrhiza inoculation and different irrigation levels on the biological yield, HI and some yield components of fennel (such as branch number per plant, umbel numbel per branch, umbellet number per umbel and seed number per umbellet) and ajwain (such as umbellet number per umbel, seed number per umbellet and 1000 seed weight) scale fern number, seed number and 1000 seed weight of ajwain were significant (p≤0.05). Inoculation with mycorrhiza, enhanced root development and resulted in the availability of moisture and nutrients, particularly phosphorus. On the other hand, these fertilizers are the cause of production of many growth regulators for the plant. The higher irrigation levels increased photosynthesis and dry matter production due to vegetative growth and photosynthesis area of the plants.

Acknowledgements
This research (044.2) was funded by Vice Chancellor of Research of the Ferdowsi University of Mashhad, which is hereby acknowledged

Keywords

Biofertilizer; Medicinal plant; Symbiosis

References

Alkire, B.H., Simon, J.E., and Pautievsky, D. 1993. Water management for Midwestern peppermint (Mentha piperita L.) growing in highly organic soils, India, USA. Acta Horticulturae 344: 544-556.

Antunes, P.M., Schneider, K., Hillis, D., and Klironomos, J.N. 2007. Can the arbuscular mycorrhizal fungus Glomus intraradices actively mobilize P from rock phosphates? Pedobiologia 51: 281-286.

Baher, Z.F., Mirza, M., Ghorbanli, M., and Rezaii, M.B. 2002. The influence of water stress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance Journal 17(4): 275-277.

Cardoso, I.M., and Kuyper, T.W. 2006. Mycorrhizas and tropical soil fertility. Agriculture, Ecosystems and Environment 116: 72-84.

Celik, I., Ortas, I., and Kilic, S. 2004. Effects of compost, mycorrhiza, manure and fertilizer on some physical properties of a Chromoxerert soil. Soil and Tillage Research 78: 59-67.

Charles, D.J., Joly, R.J., and Simon, J.E. 1999. Effects of osmotic stress on the essential oil content and composition of peppermint. Phytochemistry 29(9): 2837-2840.

Clark, R.J., and Menary, R.C. 1980. The effect of irrigation and nitrogen on the yield and composition of peppermint oil (Mentha piperita L.). Australian Journal of Agricultural Research 31(3): 489 -498.

Colom, M.R., and Vazzana, C. 2002. Water stress effects on three cultivars of Eragrostis curvula. Italy Journal of Agronomy 6: 127-132.

Copetta, A., Lingua, G., and Berta, G. 2006. Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese. Mycorrhiza 16: 485-494.

Darzi, M.T., Ghalavand, A., and Rejali, F. 2008. Evaluation the effects of mycorrhiza, vermi compost and phosphate fertilizer on flowering, biological yield and root symbiosis in fennel. Iranian Journal of Agronomy Sciences 10(1): 88-109.

El-Mougy, N.S., and Abdel-Kader, M. 2007. Antifungal effect of powdered spices and their extracts on growth and activity of some fungi in relation to damping-off disease control. Journal of Plant Protection Research 47: 267-278.

Estrada-Luna, A., and Davies, A. 2003. Arbuscular mycorrhizal fungi influence water, relations, gas exchange, abscisic acid and growth of micropropagated chile ancho pepper (Capscum annuum) plantlets during acclimatization and post-acclimatization. Journal of Plant Physiology, 160: 1073-1083.

Feng, G., Zhang, F.S., Li, X.L., Tian, C.Y., Tang, C., and Rengel, Z. 2002. Improved tolerance of maize plants to salt stress by arbuscular mycorrhiza is related to higher accumulation of soluble sugars in roots. Mycorrhiza, 12: 185-190.

Gharib, F.A., Moussa, L.A., and Massoud, O.N. 2008. Effect of compost and bio-fertilizers on growth, yield and essential oil of sweet marjoram (Majorana hortensis) plant. International Journal of Agriculture and Biology 10: 381-387.

Gosling, P., Hodge, A., Goodlass, G., and Bending, G.D. 2006. Arbuscular mycorrhiza fungi and organic farming. Agriculture, Ecosystems and Environment 113: 17-35.

Gupta, M.L., Prasad, A., Ram, M., and Kumar, S. 2002. Effect of the vesicular–arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum on the essential oil yield related characters and nutrient acquisition in the crops of different cultivars of menthol mint (Mentha arvensis) under field conditions. Bioresource Technology 81(1): 77-79.

Harrier, L.A., and Watson, C.A. 2004. The potential role of arbuscular mycorrhizal (AM) fungi in the bioprotection of plants against soil-borne pathogens in organic and/or other sustainable farming systems. Pest Management Science 60: 149-157.

Hasani, A., and Omid Beygi, R. 2002. The effects of water stress on some morphophysiological, physiological and metabolismic characteristics of ocimum. Agricultural Knowledge 3(12): 47-59. (In Persian with English Summary)

Hecl, J., and Sustrikova, A. 2006. Determination of heavy metals in chamomile flower drug- an assurance of quality control. International Symposium on chamomile Research, Development and Production. Presov, Slovakia 69 pp.

Holtzer, T.O., Archer, T.L., and Norman, J.M. 1988. Host plant suitability in relation to water stress. In: Heinrichs, E.A. (ed.), Plant Stress-Interactions, p. 111-37. Willey-lnterscience.

Jakobsen, I. 1987. Effects of VA mycorrhiza on yield and harvest index of field-grown pea. Plant and Soil 98(3): 407-415.

Jangir, R.P., and Sing, R. 1996. Effect of irrigation and nitrogen on seed yield of cumin (Cuminum cyminum). Indian Journal of Agronomy 41:140-143.

Jones, H.G., and Corlett, J.E. 1992. Current tropics in drought physiology. Journal of Agricultural Science, Cambridge 119: 291-296.

Kapoor, R., Chaudhary, V., and Bhatnagar, A.K. 2007. Effects of arbuscular mycorrhiza and phosphorus application on artemisinin concentration in Artemisia annua L. Mycorrhiza 17: 581-587.

Kapoor, R., Giri, B., and Mukerji, K.G. 2004. Improved growth and essential oil yield and quality in (Foeniculum vulgare Mill) on mycorrhizal inoculation supplemented with P-fertilizer. Bioresource Technology 93: 307-311.

Kazaee, H.R., Sabet Teimuri, M., and Nadjafi, F. 2007. The effects of irrigation regimes and seed planting amount on yield, yield components and quality of Plantago ovata L. Iranian Journal of Field Crops Research 8(5): 758-766. (In Persian with English Summary)

Khorramdel, S., Koocheki, A., Nassiri Mahallati, M., and Ghorbani, R. 2010. Inoculation effects with biofertilizers on the yield and yield components in black cumin (Nigella sativa L.). Iranian Journal of Field Crops Research 8(5): 758-766. (In Persian with English Summary)

Khorramdel, S., Koocheki, A., Nassiri Mahallati, M., and Ghorbani, R. 2008. Application effects of nitrogen and phosphorus biofertilizers on the growth indices of black cumin (Nigella sativa L.). Iranian Journal of Field Crops Research 8(5): 758-766. (In Persian with English Summary)

Kothamasi, D., Kuhand, R.C., and Babu, C.R. 2001. Arbuscular mycorrhizae in plant survival strategies. Tropical Ecology 42: 1-13.

Kravchenko, L.V., Leonova, E.I., and Tikhonovich, I.A. 1994. Effect of root exudates of non-legume plants on the response of auxin production by associated diazotrophs. Microbial Releases 2: 267-271.

Lopes-Lutz, D., Alviano, D.S., Alvino, C.S., and Kolodziejczyk, P.P. 2008. Screening of chemical composition, antimicrobial and antioxidant activites of Artemisia essential oils. Phytochemistry 69: 1732-1738.

Mahfouz, S.A., and Sharaf-Eldin, M.A. 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill.). International Agrophysics 21: 361-366.

Morone-Fortunato, I., and Avato, P. 2008. Plant development and synthesis of essential oils in micropropagated and mycorrhiza inoculated plants of Origanum vulgare L. ssp. Hirtum (Link) Ietswaart. Plant Cell, Tissue and Organ Culture 93: 139-149.

Munns, R. 2002. Comparative physiology of salt and water stress. Plant, Cell and Environment 25: 239-250.

Oehl, F., Sieverding, E., Mader, P., Dubois, D., Ineichen, K., Boller, T., and Wiemken, A. 2004. Impact of long-term conventional and organic farming on the diversity of arbuscular mycorrhizal fungi. Oecologia 138: 574-583.

Omid Beygi, R. 1995. Approaches for Production and Processing in Medicinal Plants. Vol. 1. Tarrahan-e-Nashr, Publication, Tehran, Iran 424 pp. (In Persian)

Omid Beygi, R. 1997. Approaches for Production and Processing in Medicinal Plants. Vol. 3. Fekr-e- Rooz, Publication, Tehran, Iran 283 pp. (In Persian)

Ozturk, A., Unlukara, A., Ipek, A., and Gurbuz, B. 2004. Effects of salt stress and water deficit on plant growth and essential oil content of lemon balm (Melissa officinalis L.). Pakistan Journal of Botany 36(4): 787-792.

Passioura, J.B., Condon, A.G., and Richards, R.A. 1993. Water deficit, the development of leaf area and crop productivity. In: "Water Deficit, from Cell to Community "(eds. J.A.C. Smith and H. Griffiths). Biosciences, Publication. UK. p. 253-264.

Pinior, A., Grunewaldt-Stocker, G., Von Alten, H., and Strasser, R.J. 2005. Mycorrhizal impact on drought stress tolerance of rose plants probed by chlorophyll a fluorescence, praline content and visual scoring. Mycorrhiza 15: 596-605.

Razmjoo, K., Heydarizadeh, P., and Sabzalian, M.R. 2008. Effect of salinity and drought stresses on growth parameters and essential oil content of Matricaria chamomila. International Journal of Agriculture and Biology 10: 451-454.

Richter, J., Stutzer, M., and Schellenberg, I. 2005. Effects of mycorrhization on the essential oil content and composition of aroma components of marjoram (Marjorana hortensis), thyme (Thymus vulgaris L.) and ajwain (Trachyspermum carvi L.). 36th International Symposium on Essential Oils, 4-7 September, Budapes, Hungary.

Sardans, J., Roda, F., and Penuelas, J. 2005. Effect of water and a nutrient pulse supply on Rosemarinus officinalis growth, nutrient content and flowering in the field. Environmental and Experimental Botany 53: 1-11.

Shabih, F., Farooqi, A.H.A., Ansari, S.R., and Sharma, S. 1999. Effect of water stress on growth and essential oil metabolism in Cymbopogon martinii cultivars. Journal of Essential Oil Research 11: 491-496.

Singh, M., Sharma, S., and Ramesh, S. 2002. Herbage, oil yield and oil quality of patchouli [Pogostemon cablin (Blanco) Benth.] influenced by irrigation, organic mulch and nitrogen application in semi-arid tropical climate. Industrial Crops and Products 16(2): 101-107.

Surendra, S.R., Tomar, K.P., Gupta, K.P., Mohd, A., and Nigam, K.B. 1994. Effect of irrigation and fertility levels on growth and yield of coriander (Coriandrum sativum). Indian Journal of Agronomy 39: 442-447.

Uhlmann, E., Görke, C., Petersen, A., and Oberwinkler, F. 2006. Arbuscular mycorrhizae from arid parts of Namibia. Journal of Arid Environments 64: 221-237.

Vance, C.P., Uhde-Stone, C., and Allan, D.L. 2003. Phosphorus acquisition and use: critical adaptation by plants for securing a non- renewable resource. New Physiology 157: 423-447.