Effects of Single and Combined Application of Organic, Biological and Chemical Fertilizers on Quantitative and Qualitative Yield of Coriander (Coriandrum sativum)

علوم باغبانی

Article 2, Volume 29, Issue 4, November 2015, Pages 486-500   

Document Type: Research Article

DOI: 10.22067/jhorts4.v29i4.51510

Authors

Mahsa Aghhavani Shajari^{* 1}; Parviz Rezvani Moghaddam²; Reza Ghorbani¹; Mahdi Nasiri Mahalati

¹Ferdowsi University of Mashhad

²Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad

Abstract

Introduction: Medicinal plants were one of the main natural resources of Iran from ancient times. Coriander (Coriandrum sativum L.) is from Apiaceae family that it has cultivated extensively in the world. Management and environmental factors such as nutritional management has a significant impact on the quantity and quality of plants. Application of organic fertilizers in conventional farming systems is not common and most of the nutritional need of plants supply through chemical fertilizers for short period. Excessive and unbalanced use of fertilizers in the long period, reduce crop yield and soil biological activity, accumulation of nitrates and heavy metals, and finally cause negative environmental effects and increase the cost of production. The use of bio-fertilizers and organic matter are taken into consideration to reduce the use of chemical fertilizers and increase the quality of most crops. Stability and soil fertility through the use of organic fertilizers are important due to having most of the elements required by plants and beneficial effects on physical, chemical, biological and soil fertility. Therefore, the aim of this research was to evaluate the effects of organic, biological and chemical fertilizers on quality and quantity characteristics of coriander.
Materials and Methods: In order to study the effects of single and combined applications of organic, biological and chemical fertilizers on quantitative and qualitative characteristics of Coriander (Coriandrum sativum), an experiment was conducted based on a randomized complete block design with three replications and 12 treatments at Research Station, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran, in - 2011. Treatments included: (1) mycorrhizae (Glomus mosseae) (2) biosulfur (Thiobacillus sp.), (3) chemical fertilizer (NPK), (4) cow manure, )5( vermin compost, )6( mycorrhizae + chemical fertilizer, )7( mycorrhizae + cow manure, )8( mycorrhizae + vermicompost, )9( biosulfur + chemical fertilizer, )10( biosulfur + cow manure, )11( biosulfur + vermin compost and )12( control. Glomus mosseae was used at -2 cm depth below the seed. Chemical fertilizer, vermicompost (17 t. ha-1) and cow manure (60 t.ha-1) were used at 30 cm depth . Coriander was planted through furrow in mid-March 2010. The distance between rows was 60 cm and irrigation was done once a week. The common cultivation practices used for this plant and no pesticides or herbicides were used during the growing season. Coriander harvested in early July 2011 when the plants became yellow. Then, plant indices were measured in laboratory which included plant height, the number of lateral branch, the number of umbels per plant, the number of seed per umbels and plant, hundred seed weight, biological and economical yield, harvest index, essential oil percentage and essential oil yield. Statistical analysis of the data was performed by SAS 9.1and means comparison were compared by Duncan’s multiple range (P < 0.05).
Results and Discussion: The results showed that the highest number of lateral branch per plant (6.6) was obtained in biosulfur + cow manure treatment. Also, the highest number of umbel per plant (19) and number of umbelet per umbel (5) were observed in mycorrhizae treatment. Mycorrhizae by improving the nutritional status of plants, soil microbial community strengthening and protecting plants against pathogens increases plant growth and development. The highest number of seeds per umbel (21.6), the number of seeds per plant (366), seed yield (1468 kg,ha-1) and harvest index (55%) were obtained in mycorrhizae + chemical fertilizer treatment. Combined application of biosulfur with chemical fertilizer had a noticeable effect on 100-seed weights and biological yield indices. Ali et al., (2009) concluded that application of intermediate levels of nitrogen fertilizer and mycorrhizae increased biological yield of coriander, they reported that the use of nitrogen fertilizer will speed up the establishment of mycorrhizae. Studies showed that the combined use of chemical fertilizer and manure improved crop yield by increasing the efficiency ofnutrient absorption for plants. The highest essential oil percentage and essential oil yield were observed in mycorrhizae and mycorrhizae with chemical fertilizer treatments, respectively (0.2% and 2602 g.ha-1). Also, the essential oil yield was 7 times more in mycorrhizae+chemical fertilizer than biosulphur treatment. Combined application of cow manure and chemical fertilizer increased quality of indices of Ajowan. It seems that combined application of organic and bio fertilizers and cow manure improve soil physical and chemical properties, increase the availability of nutrients and finallyincrease the yield and quality of plants.
Conclusions: Overall, results of this experiment showed that application of biological fertilizer especially mycorrhizae had a significant effect on improving quantitative and qualitative yield of Coriander. Furthermore, the combined application of mycorrhizae with organic and chemical fertilizer had better effects than their single application.

Keywords

Biosulfur; Biological yield; Essential oil; Essence Yield; Harvest index; Mycorrhiza

References

1- Adediran J.A., Taiwo L.B., Akande M.O., Sobulo R.A., and Idowu O.J. 2004. Application of organic and inorganic fertilizer for sustainable maize and cowpea yields in Nigeria. Journal of Plant Nutrition, 27: 1163-1181.

2- Ahmadian H., Ghanbari A., and Ghalavi M. 2006. Effect of animal manure on quantitative and qualitative yield and chemical composition of essential oil in cumin (Cuminum cyminum). Journal of Iranian Field Crop Research, 4(2): 207-216. (In Persian with English Summary)

3- Akbari Nia A., Ghalavand A., Tahmasebi Z., Sefidkon F., Sharifi Ashoorabadi A., and Rezaei M.b. 2002. Effect of different feeding systems on yield and essential oil content of seeds Ajowan. Medicinal and Aromatic Plants Research, 18: 89-109. (In Persian with English Summary)

4- Akbari Nia A., Daneshian J., and Mohammadbeigi F. 2006. Effect of nitrogen fertilizer and plant density on seed yield, essential oil and oil content of Coriandrum sativum L.. Iranian Journal of Medicinal and Aromatic Plants, 22(4): 410-419. (In Persian with English Summary)

5- Ali F.S., Zayed G., Saad O.A., and Abdul-Mohsen H. 2009. Optimisation of nitrogen fertilizer level for maximum colonization of mycorrhizae on roots of Coriander plants. African Crop Science Conference Proceeding, 9: 117-122.

6- Aliabadi Farahani H., Arbab A., and Abbaszadeh B. 2008. The effects of super phosphate triple, water deficit stress and Glomus hoi biological fertilizer on some quantity and quality characteristics of Coriandrum sativum L.. Iranian Journal of Medicinal and Aromatic Plants, 24(1): 18-30. (In Persian with English Summary)

7- Allievi L., Marchesisni A., Salardi C., Piano V., and Ferrari A. 1993. Plant quality and soil residual fertility six years after a compost treatment. Bioresource Technology, 43: 85-89.

8- Ardakani M., Sani B., Noormohammadi Gh., Khosravi H., and Farahbakhsh A. 2007. Efficiency comparison of inoculated biological fertilizers on production and yield of soybean. Abstract of 2nd National Congress of Ecological Agriculture of Iran. 17 and 18 October 2007, Gorgan. (In Persian)

9- Arganosa G.C., Sosuskli F.W., and Slikard A.E. 1998a. Seed yield and essential oil of biennial caraway grown in Western Canada. Journal of Herbs, Spices and Medicinal Plants, 1(9): 9-17.

10- Arganosa G.C., Sosuskli F.W., and Slikard A.E. 1998b. Seed yield and essential oil of northern-grown coriander (Coriandrum sativum L.). Journal of Herbs, Spices and Medicinal Plants, 6(2): 23-32.

11- Arpana J., and Bagyaraj D.J. 2007. Response of kalmegh to an Arbuscalar Mycorrhizal fungus and a plant growth promoting rhizomicroorganism at two levels of phosphorus fertilizer. American-Eurasian Journal Agriculture Science, 2(1): 33-38.

12- Aseri G.K., Jain N., Panwar J., Rao A.V., and Meghwal P.R. 2008. Biofertilizers improve plant growth, fruit yield, nutrition, metabolism and rhizosphere enzyme activities of Pomegranate (Punica granatum L.) in Indian Thar Desert. Scientia Horticulturae, 117: 130–135.

13- Atiyeh R.M., Arancon N., Edwards C.A., and Metzger J.D. 2002. Incorporation of earthworm processed organic wastes into greenhouse container media for production of marigolds. Bioresource Technology, 81(2): 103-108.

14- Azeez J.O., Van Averbeke W., and Okorogbona A.O.M. 2010. Differential responses in yield of pumpkin (Cucurbita maxima L.) and nightshade (Solanum retroflexum Dun.) to the application of three animal manures. Bioresource Technology, 101: 2499–2505.

15- Azzaz N.A., Hassan E., and Hamed E.H. 2009. The chemical constituent and vegetative and yielding characteristics of fennel plants treated with organic and bio-fertilizer instead of mineral fertilizer. Australian Journal of Basic and Applied sciences, 3(2): 579-587.

16- Bachman G.R., and Metzger J.D. 2008. Growth of bedding plants in commercial potting substrate amended with vermicompost. Bioresource Technology, 99: 3155–3161.

17- Backhaus G.F., Häggblom P., and Nilsson L.O. 1986. The influence of vesicular-Arbuscular mycorrhizae on biomass production in willow. Canadian Journal Forestry Research, 16: 103–108.

18- Bagyaraj D.J., and Varma A. 1995. Interactions between Arbuscular mycorrhizal fungi and plants: Their importance in sustainable agriculture in arid and semiarid tropics. Advances in Microbial Ecology, 14: 119-122.

19- Bauer A., and Black A.L. 1994. Quantification of the effect of soil organic matter content on soil productivity. Soil Science Society of America, 58: 185-193.

20- Besharati kelaye H., and Salehrastin N. 2000. The effects of sulfur and inoculants of Thiobacillus bacteria on the amount of iron and zinc absorption by corn in a greenhouse. Journal of Soil and Water, 7(12): 63-72. (In Persian)

21- Bethlenfalvay G.J., Andrade G., and Azcon-Aquilar C. 1997. Plant and soil response to mycorrhizal fungi and rhizobacteria in nodulated or nitrate-fertilized peas (Pisum sativum L.). Biology Fertilizer Soils, 24: 164–168.

22- Cala V., Cases M.A., and Walter I. 2005. Biomass production and heavy metal content of Rosmarinus officinalis grown on organic waste-amended soil. Journal Arid Environment, 62: 401-412.

23- Copetta A., Lingua G., and Berta G. 2006. Effects of three AM fungi on growth, distribution of glandularhairs, and essential oil production in Ocimum basilicum L. var. Genovese. Mycorrhiza, 16: 485-494.

24- Darzi M.T., Galavand A., Rejali F., and Sefid kon F. 2007. Effect of biofertilizers application on yield and yield components in fennel (Foeniculum vulgare). Iranian Journal of Medicinal and Aromatic Plants, 22(4): 276-292.

25- Delate K. 2000. Heenah mahyah student form herb trail. Leopold center for sustainable agriculture. Annual Reports, Iowa State University. Ames, IA

26- Demir S. 2004. Influence of Arbuscular mycorrhiza on some physiological growth parameters of pepper. Turkish Journal Biology, 28: 85-90.

27- Dierchesen A. 1996. Promoting the Conservation and Use of Underutilized and Neglected Crop. Coriander, International Plant Genetic Resources Institute (IPGRI), Italy. 82 pp.

28- Ebadi M.T., Fallahi J., Azizi M., and Rezvani Moghaddam P. 2007. The effects of application of organic fertilizer on growth parameters and yield of two breeding cultivars of chamomile. The First National Conference of Management and Developing of Sustainable Agriculture in Iran, Ahvaz, 3-5 December 2007, p. 129-135. (In Persian)

29- Fallah A. Ghalavand M., and Khajehpour R. 2007. Effects of animal manure incorporation methods and its integration with chemical fertilizer on yield and yield components of maize (Zea mays L.) in Khorramabad, Lorestan. Journal of Sciences and Technology of Agriculture and Natural Resource, 40(11): 233-243. (In Persian with English Summary)

30- Fallahi J., Koocheki A., and Rezvani Moghaddam P. 2008. The effect of organic fertilizers on quantity index and the amount of essential oil and chamazulene in chamomile. Agriculture Research: Water, Soil and Plant in Agriculture, 8(1): 157-168. (In Persian with English Summary)

31- Fallahi J., Koochaki A., and Rezvani Moghaddam P. 2009. Investigating the effects of biological fertilizers on qualitative and quantitative of chamomile. Journal of Iranian Field Crop Research, 7(1): 127-135. (In Persian with English Summary)

32- Fatma A.G., Lobna A.M., and Osman N.M. 2008. Effect of compost and biofertilizers on growth, yield and essential Oil of sweet marjoram (Majorana hortensis) Plant. International Journal of Agriculture and Biology, 10(4): 381–387.

33- Forooghi far H., and Poorkasmaei M.A. 2002. Science and Soil Management (Volume I) (Translation). Ferdowsi University of Mashhad Publisher, Mashhad.

34- Ghazi A., Nehad A., and Yahia-Othman. 2007. Application of mycorrhizae fungi to improve drought tolerance in two onion cultivars. African Crop Science Society, 8: 1-5.

35- Ghone I.M., and Abed-Razik A.H.1999. Effect of biofertilisation under different nitrogen levels on growth, yield and chemical contents of potato plants. Advanced Journal of Agriculture Research, 4(2): 757-769.

36- Ghost B.C., and Bhat R. 1998. Environmental hazards of nitrogen loading in wetland rice fields. Environmental Pollut, 102: 123–126.

37- Gujar S.M., Warade A.D., Anjali M., and Paltankar D.H. 2005. Effect of dates of sowing and nitrogen levels on growth, seed yield and quality of Coriander. Crop Research, 29(2): 288-291.

38- Imre E., Somssich I.E., and Hahlbrok K. 1998. Pathogen defense in plant-a paradigm of biological complexity. Trends in Plant Science, 3(3): 86–90.

39- Iranipoor R., Malakooti M.J., Abedi A., Sajjadi A., and Ghafoorian H. 2007. The main effects of phosphate, sulfur and Thiobacillus bacteria on crop yield and residual effects of it on crop yield of barley. Journal of Soil and Water Sciences, 21(2): 195-205. (In Persian)

40- Jahan M. 2004. Evaluation of ecological aspects of intercropping of chamomile and calendula with manure. M.Sc Thesis, agronomy, Ferdowsi University of Mashhad. (In Persian)

41- Jahan M., Nassiri Mahallati M., Salari M.D., and Ghorbani R. 2000. The effects of application time of manure and biological fertilizers on quality and quantity characteristics of medicinal pumpkin. Iranian Journal of Field Crops Research, 8(4): 726-7347. (In Persian)

42- Jeffries P., Gianinazzi S., Perotto G., Turnau K., and Bareae J. 2003. The contribution of Arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biology and Fertility of Soils, 37: 1-16.

43- Jha A.K. Netra P., and Sexena A.K. 2005. Screening of Vesicular Arbuscular mycorrhizas for onion. Indian Journal of Horticulture, 62(4): 411-412.

44- Johansen A., Jakobsen I., and Jensen E.S. 1994. Hyphal N transport by a vesicular Arbuscular mycorrhizal fungus associated with cucumber grown at three nitrogen levels. Plant and Soil, 160: 1–9.

45- Kalra A. 2003. Organic cultivation of medicinal and aromatic plants. A hope for sustainability and quality enhancement. Journal of Organic Production of Medicinal, Aromatic and Dye-Yielding Plants (MADPs). FAO.

46- Kanchikerimath M., and Singh D. 2001. Soil organic matter and biological properties after 26 years of maize-wheat-cowpea cropping as affected by manure and fertilization in a Cambisol India. Agriculture, Ecosystems and Environment, 86:155-162.

47- Kapoor R., Giri B., and Mukerji K.G. 2002. Mycorrhization of coriander (Coriandrum sativum) to enhance the concentration and quality of essential oil. Journal of the Science of Food and Agriculture, 82(4): 339- 342.

48- Kapoor R., Giri B., and Mukerji K. G. 2004. Improved growth and essential oil yield and quality in Foeniculum vulgare mill on mycorrhizal inoculation supplemented with P-fertilizer. Bioresource Technology, 93: 307–311.

49- Karimizarchi M., and Kalbasi M. 1998. Effect of aeration and mixing on process of composting and compost quality produced from municipal waste. The Sixth Congress of Soil Science, Ferdowsi University of Mashhad, pp: 7.

50- Karthikeyan B., Abdul Jaleel C., Lakshmanan G.M.A., and Deiveekasundaram M. 2008. Studies on rhizosphere microbial diversity of some commercially important medicinal plants. Colloids and Surfaces B: Biointerfaces, 62:143–145.

51- Khandan A. 2004. Effects of organic and chemical fertilizers on soil physical and chemical properties of Plantago pslliom. M.Sc Thesis of pedology, Ferdowsi University of Mashhad. (In Persian with English Summary)

52- Khorassani R. 2000. Phosphorus uptake efficiency in corn, sugar beet and groundnut. Journal of Water and Soil, 24(1): 180-188. (In presian)

53- Koocheki A., Tabrizi L., and Ghorbani R. 2008. Effect of biofertilizers on agronomic and quality criteria of Hyssop (Hyssopus officinalis). Iranian Journal of Field Crops Research, 6: 126-136. (In Persian with English Summary)

54- Kovarova M., Frantik T., Koblihova H., Bartunkova K. Nyvltova Z., and Vosatka M. 2011. Effect of clone selection, nitrogen supply, leaf damage and mycorrhizal fungi on stilbene and emodin production in knotweed. BMC Plant Biology, 11:98-111.

55- Kumar T.S., Swaminathan V., and Kumar S. 2009. Influence of nitrogen, phosphorus and biofertilizer on growth, yield and essential oil constituents in Raton crop of davana (Artemisia pallens). Electronic Journal of Environmental, Agricultural and Food Chemistry, 8(2): 86-95.

56- Lebaschi M.H., Matin A., and Amin, GH. 2001. The effect of organic and chemical fertilizers on the yield and composition of Hypericum. Iranian Journal of Medical and Aromatic Plants, 10: 53-63. (In Persian)

57- Mahfouz S.A., and Sharaf-Eldin M.A. 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill.). International Agrophysics, 21: 361-366.

58- Majidian M., Ghalavand A., Karimaian N.F., and Kamgar Haghighi, A.A. 2008. Effects of water stress, nitrogen fertilizer, manure and combination of nitrogen fertilizer and manure on yield, yield components and water use efficiency of SC 704 Corn. Science and Technology of Agriculture and Natural Resources, 12(44): 417-432. (In Persian)

59- Malakuti M.J., and Tehrani M.M. 1999. The Role of Micronutrients in Increasing Yield and Improving of Quality of Agriculture Production. Tarbiat Modares University Publishing. pp 190.

60- Mallanagouda B. 1995. Effects of N. P.K and FYM on growth parameters of onion, garlic and coriander. Journal of Medic and Aromatic Plant Science, 4: 916-918.

61- Mohamadi Ariya M., Lakziyan A., and Hagh Nia G.H. 2010. The effects of innoculum of Thiobacillus and Asperjillus on growth of Zea mays. Journal of Iranian Field Crop Research, 8(1): 82-89. (In Persian)

62- Moradi R. Rezvani Moghaddam P. Nasiri Mahallati M., and Lakzian A. 2009. The effect of application of organic and biological fertilizers on yield, yield components, and essential oil of Foeniculum vulgare (Fennel). Journal of Iranian Field Crop Research, 7(2): 625-635. ( In Persian with English Summary)

63- Mostafavian S.R., Pirdashti H., Ramzanpour M.R., Andarkhor A.A., and Shahsavari A. 2008. Effect of mycorrhizae, Thiobacillus and sulfur nutrition on the chemical composition of soybean [Glycine max (L.)] Merr. seed. Pakistan Journal of Biological Sciences, 11(6): 826-835.

64- Neffati M., Sriti J., Hamdaoui G., Kchouk M.E., and Marzouk B. 2011. Salinity impact on fruit yield, essential oil composition and antioxidant activities. Food Chemistry, 124: 221- 225.

65- Omidbaigi R. 2005. Production and Processing of Medicinal Plants. Vol: 1. Tehran, Astane Qodse Razavi Publication. 283 pp.

66- Paksoy M., and Aydin C. 2004. Some physical properties of edible squash (Cucurbita pepo L.) seeds. Journal of Food Engineering, 65: 225-231.

67- Paradi I., Bratek Z., and Lang F. 2003. Influence of Arbuscular mycorrhiza and phosphorus supply on polymine content, growth and photosynthesis of Plantago anceolata. Biologia Plantarum, 46:563-569.

68- Parmar D.K., and Sharma T.R. 1998. Integrated nutrient supply system for DPPG8, vegetable pea (Pisum sativum var aravense) in dry temperature zone of Himachal Pradesh. Indian Journal Agriculture Science, 68: 247-253.

69- Rahimi A.R., Mashayekhi K., Hemmati Kh., and Dordipour E. 2009. Effect of salicylic acid and mineral nutrition on fruit yield and yield components of coriander (Coriandrum sativum L.). Journal of Plant Production, 16(4): 149-156. (In Persian with English Summary)

70- Ramadan M.F., and Morsel J.T. 2003. Analysis of glycolipids from black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.) and niger (Guizotia abyssinica Cass.) oilseeds. Food Chemistry, 80:197–204.

71- Rivera-Cruz M.C., Narcıa A.T., Ballona G.C., Kohler J., Caravaca F., and Rold A. 2008. Poultry manure and banana wastes are effective biofertilizer carriers for promoting plant growth and soil sustainability in banana crops. Soil Biology and Biochemistry, 40: 3092–3095.

72- Rosa M.C., Muchovaj J., and Alvarez V.H. 1989. Temporal relation of 17 phosphorus fraction in an oxisol amended Phosphate rock and Thiobacillus thiooxidans. Soil Science Society American Journal, 53: 1096-110.

73- Sabahi, H., Ghalavand, A., Modares sanavi, A.M., and Asgharzadeh, A. 2008. Comparing the effects of integrated and conventional fertilization systems on canola (Brassica napus) yield and chemical properties of soil, Journal of Soil and Water, 22(2): 1-15. (In Persian with English Summary)

74- Saleh E.A., Nokhal T.H., El-Borollosy M.A., Fendrik I., Sharaf M.S., and El-sawy M. 1998. Effectiveness of dual inoculation with diazotrophs and vesicular Arbuscular mycorrhizae on growth and medicinal compounds of Datura stramonium. Arab Universities Journal of agricultural Sciences, 6(2): 343-355.

75- Salimpour S., Khavazi K., Nadian H., Besharati H., and Miransari M. 2010. Enhancing phosphorous availability to canola (Brassica napus L.) using P solubilizing and sulfur oxidizing bacteria. Australian Journal of Crop Science, 4(5): 330-334.

76- Schofield P.E., Gregg P., and Syers J.K. 1981. Biosuper as a phosphate fertilizer (a glasshouse evaluation). New Zealand Journal of Experimental Agriculture, 9: 63-97.

77- Sefid kon, F. 1998. Study of shoots and fruit essential oil of coriander. Iranian Journal of Medical and Aromatic Plants, 13: 32-38. (In Persian)

78- Shaalan M.N. 2005. Effect of compost and different sources of biofertilizers, on borage plants (Borago officinalis). Egypt Journal of Agriculture Research, 83(1): 271.

79- Sharifi ashoorabadi A., Ghalavand A., Noormohammadi GH., Matin A., Amin Gh. Babakhanloo P., Lebaschi M.H., and Sefidkon F. 2001. Effect of organic and chemical fertilizers on the performance of fennel. Iranian Journal of Medical and Aromatic Plants, 7: 3-26. (In Persian)

80- Termorshuizen A.J., and Ket P.C. 1993. The effects of fertilization with ammonium and nitrate on mycorrhizal seedlings of Pinus sylvestris. Agriculture Ecosystems and Environment, 28(1): 497-501.

81- Uhart S.A., and Andrade F.H. 1995a. Nitrogen deficiency in maize. I. Effect on crop growth, development to dry matter-partitioning, and kernel set. Crop Science, 35:1376-1383.

82- Uhart S.A., and Andrade F.H. 1995b. Nitrogen deficiency in maize. II. Effect on crop carbon- nitrogen interaction effect on kernel number and grain yield. Crop Science, 35: 1384-1389.

83- Vazquez M.M., Barea J.M., and Azcon R. 2002. Influence of Arbuscular mycorrhizae and a genetically modified strain of Sinorhizobium on growth, nitrate reductase activity and protein content in shoots and roots of Medicago sativa as affected by nitrogen concentrations. Soil Biology and Biochemistry, 34: 899-905.

84- Vildova A., and Stolcova M. 2006. Cultivation technology of chamomile (Matricaria recutita L.) in organic agriculture. 1nd international Symposium on Chamomile Research, Development and Production. Slovak Republic, Prosov, pp 50.

85- Wu S.C., Cao Z.H., Li Z.G. Cheung K.C., and Wong M.H. 2005. Effects of biofertilizers containing N-fixer, P and K solubilizer and AM fungi on maize growth: a greenhouse trail. Geoderma, 125:155-166.

86- Yalcintas G. 1995. The effect of sowing dates and levels of nitrogen fertilizer on yield and some agricultural characteristics of Coriander. Master Thesis, Ondokuz Mayis University, Institute of Natural and Applied Sciences. Department of Agronomy, Turkey.