)Brief Report) First Report of Detection on Cucumber green mottle mosaic virus (CGMMV) by Serological and Molecular Methods in Razavi and Northern Khorasan Provinces

پژوهش های حفاظت گیاهان ایران

Article 14, Volume 28, Issue 2 - Serial Number 27, June 2014, Pages 263-266   

Document Type: Research Article

DOI: 10.22067/jpp.v28i2.40010

Authors

Z . Moradi^* ; B. Jafarpour; Mohammad Ali Sabokkhiz

Abstract

Virus infections are a major limiting factor in cucurbit production in Iran. Cucumber green mottle mosaic virus (CGMMV) infects many cucurbit species in the world and causing mottle and systemic mosaic symptoms on cucurbitaceous plant leaves. During 2008-2009, from different Cucurbitaceous species fields and cucumber greenhouses in Razavi and Northern Khorasan provinces, 220 leaf samples with CGMMV-like symptoms were collected and brought to the laboratory under standard condition. The identity of virus was established by the studying host rang symptoms and serological assay. By using DAS-ELISA technique 47 samples showed infection with CGMMV. Sap of infected samples inoculated on a number of indicator plants and presence of CGMMV was confirmed by observation of specific symptoms and DAS-ELISA results. To confirm the results of ELISA test, total RNA was extracted using RNXTM(-Plus) solution and synthesis of cDNA followed by Touch-down PCR using specific primer for amplification of coat protein gene. PCR product was electrophoresed in a 1.7% agarose gel in TBE buffer and the band of 420 bp related to CGMMV was detected .This is the first report of occurrence of CGMMV in different regions of Razavi and Northern Khorasan provinces.

Keywords

Cucumber green mottle mosaic virus (CGMMV); DAS-ELISA; RT-PCR; Razavi and Northern Khorasan provinces