Study of Blood Metabolites Changes of Purebred Ghezel and Crossbred Arkhar Merinos × Ghezel Ewes during Late Pregnancy

پژوهشهای علوم دامی ایران

Article 5, Volume 8, Issue 2 - Serial Number 26, June 2016, Pages 392-402   

Document Type: Physiology

DOI: 10.22067/ijasr.v8i2.45117

Authors

Leila Ahmadzadeh¹; Ali Hossein khani^{* 2}; Saman Saedi³; Hossein Daghigh Kia³; Malihe Dadashi³; Jaber Jafarzadeh⁴

¹University of Tabriz

²Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

³Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Ira

⁴University Of Tabriz

Abstract

Introduction As pregnancy progresses, nutrient requirements of fetus and thereby ewe increases and some changes in the levels of blood metabolites including glucose, cholesterol and total protein may occur. During gestation, maternal tissues contribute to supplying energy that required for fetus resulting in changes of ewe blood metabolites; however other factors such as breed, age, feeding type of ewes during gestation and season may influence them. The study of blood metabolic changes in different phases of reproductive cycle can be helpful in detecting abnormal situations of ewes and preventing of metabolic disorders such as pregnancy toxemia and fatty liver syndrome. There is little information about the effect of different genotypes on blood metabolites and the occurrence of metabolic disorders in late pregnancy. Therefore the aim of the present study was to determine changes in blood metabolites of purebred Ghezel and ArkharMerino× Ghezel crossbred ewes during late pregnancy and effect of crossbreeding of Iranian purebred sheep with Merino sheep on metabolite level changes in these two groups of sheep and study of susceptibility to metabolic disease in late pregnancy.
Materials and Methods In the present study, fifty five pregnant Ghezel ewes (36 singles and 15 twins) and 34 pregnant crossbred ewes (20 singles and 14 twins) were used. Estrus synchronization of all ewes was done using CIDR. CIDR were removed 14 days later and all ewes were injected PMSG intramuscularly and then mated with rams. All of the ewes were grazing in the pasture during pregnancy, but in the last two months of pregnancy, feeding of ewes was manually. Blood samples were collected by vacuum tubes during four hours after feeding from the jugular vein of ewes on 15 days prior to mating period and on days 90, 120 and 140 of the pregnancy. Blood samples were centrifuged with 4000 rpm for 12 minutes to extract blood serum and then sera were frozen in -20oC until further analysis of metabolites. Measurement of blood metabolites, including total protein, calcium, glucose, cholesterol, blood urea nitrogen and albumin was done by spectrophotometer set. SAS software (2003) was used for statistical analysis. Mixed procedure of SAS software was used for statistical analysis and Tukey- Kramer test was applied for comparison of means. T-test was used for comparison of blood metabolites between ewes with single and twin lambs.
Results and Discussion The results showed that the blood glucose, total protein, albumin and calcium on days 90, 120 and 140 of pregnancy were less than 15 days prior to mating but blood urea nitrogen and cholesterol levels were increased. These variations could originate from fetus metabolism since fetus requirements for tissue growth, muscles and bones increases during pregnancy, which should be supplied from maternal body reserves. The comparison of blood metabolites on the both crossbred and purebred pregnant ewes showed that in the late pregnancy, twin pregnant ewes had less glucose, total protein and calcium and more blood urea nitrogen and cholesterol compared with single pregnant ewes because there is a higher fetus requirement for twin fetuses during gestation. Our results also showed that glucose, total protein, albumin and blood urea nitrogen ‏levels of crossbred ewes were higher than purebred Ghezel ewes on 15 days prior to mating period. This may be due to different genetic potential of these breeds. During the gestation period, levels of mentioned metabolites except for blood urea nitrogen‏ were higher in both single and twin crossbred pregnant ewes than purebred Ghezel ewes significantly. It is seemed that these differences may be related to variation in the genetic potential of studying animals.
Conclusion The results of present study showed that there were no signs of pregnancy toxemia in the two experimental groups; however some differences among blood metabolites of purebred and hybrid ewes were found, which may originate from genetic potential of these two groups. These differences revealed that hybrid ewes are more resistant against blood metabolite changes during late pregnancy. Study of fluctuations in the blood metabolites during several stages of pregnancy can help us to determine abnormal metabolic cases and prediction of metabolic disorders such as pregnancy toxemia or ketosis, fatty liver syndrome and disease relating to fetus and ewe metabolism.

Keywords

Arkhar-Merino×Ghezel Ewes; Blood metabolites; Pregnancy Toxemia; Purebred Ghezel Ewes

References

1- Alimohammadi, R., and H. Aliarabi. 2013. Effect of different levels of Selenium additive on performance, blood metabolites and digestibility of nutrients in Mehrban male lambs. Iranian Journal of Animal Science Research, 5(1):48-55. (In Persian).

2- Al-Qudah, K. M. 2011.Oxidant and antioxidant profile of Hyperketonemia ewes affected by pregnancy toxaemia. Veterinary and Clinical Pathology, 40(1): 60–65.

3- Antunovic, Z., D. Sencic., M. Sperada., and B. Liker. 2002. Influence of the season and the reproductive status of ewes on blood parameters. Small Ruminants Research, 45(1): 39-44.

4- Azab, M. E., and H. A. Abdel-Maksoud. 1999. Changes in some hematological and biochemical parameters during prepartum and postpartum periods in female Baladi goats. Small Ruminant Research, 34(1):77-85.

5- Balıkc, A., E. Yıldız., and F. Gurdogan. 2005. Blood metabolite concentrations during pregnancy and postpartum in Akkaraman ewes. Small Ruminants Research. 67 (2): 247–251.

6- Bani Ismail, Z. A., A. M. Al-Majali., F. Amireh., and O. F. Al-Rawashdeh. 2008. Metabolic profiles in goat do in late pregnancy with and without subclinical pregnancy toxemia. Veterinary and Clinical Pathology, 37(4):434-437.

7- Beskow, A. P, C. G. Fernandes., and G. Leipnitz. 2008. Influence of ketone bodies on oxidative stress parameters in brain of developing rats in vitro. Metabolic Brain Disease. 23(4):411-425.

8- Blum, J. W., C. F. Romberg., K. G. Johnson., and D. S. Kronfeld. 1972. Calcium (ionized and total), magnesium, phosphorus and glucose in plasma from parturient cows. American Journal of Veterinary Research. 33(1): 51-56.

9- El-Sherif, M. M. A., and F. Assad. 2001. Changes in some blood constituents of Barki ewes during pregnancy and lactation under semiarid conditions. Small Ruminant Research. 40(3): 269–277.

10- Firat, A., and A. O¨zpinar. 1996. The study of changes in some blood parameters (glucose, urea, bilirubin, AST) during and after pregnancy in association with nutritional conditions and litter size in ewes. Turkey Journal of Veterinary and Animal Science, 20(2):387–393.

11- Gojnic, M., S. Petkovic., M. Papic., T. Mostic., K. Jeremic., Z. Vilendecic., and S. Djordjevic. 2004. Plasma albumin level as an indicator of severity of preeclampsia. Clinical and Experimental Obstetrics and Gynecology, 31(3): 209–210.

12- Hamadeh, M. E., H. Bostedt., and K. Failing. 1996. Concentration of metabolic parameters in the blood of heavily pregnant and nonpregnant ewes. Berliner und Münchener Tierärztliche Wochenschrift Journal, 109(3):81–86.

13- Jainudee, M. R., and E. S. E. Hafez. 1994.Gestation, prenatal physiology and parturition. In: Hafez, E.S.E. (Ed.), Reproduction in Farm Animals. Lea and Febiger, Philadelphia, Pp: 247–283.

14- Kaneko, J. J., J. W. Harvey., and M.L. Bruss. 2008. Clinical biochemistry of domestic animals. 5th edn. Academic Press, USA, chapter’s 3, 4 and appendices no.VIII.

15- Moghaddam, G., and A. Hassanpour. 2008. Comparison of blood serum glucose, beta hydroxybutyric acid, blood urea nitrogen and calcium concentrations in pregnant and lambed ewes. Journal of Animal and Veterinary Advances, 7(3):308-311.

16- Nazifi, S., M. Saeb., and S. M. Ghavami. 2002. Serum lipid profile in Iranian fat-tailed sheep in late pregnancy, at parturition and during the post-parturition period. Journal of Veterinary Medicine Series, 49(1):9–12.

17- Piccione, G., G. Caola., C. Giannetto., F. Grasso., S. Calanni Runzo., A. Zumbo., and P. Pennisi. 2009. Selected biochemical serum parameters in ewes during pregnancy, post-parturition, lactation and dry period.Animal Science Papers and Reports, 27(4): 321-330.

18- Ramin, A. G., S. Asri., and R. M Ajdani. 2005. Correlations among serum glucose, betahydroxybutyrate and urea concentration in non–pregnant ewes. Small Ruminant Research Journal, 57(2):265-269.

19- Rook, J. S. 2000. Pregnancy toxemia of ewes does and beef cow. Veterinary Clinics of North America: Food Animal Practice, 16(2):293–317.

20- Robinson, J. J., J. A. Rooke., and T. G. McEvoy. 2002. Nutrition for Conception and Pregnancy In: Sheep nutrition by M. Freer and H. Dove, CAB international, chapter 9: 198-211.

21- Schlumbohm, C., and J. Harmeyer. 2004. Hyperketonemia impairs glucose metabolism in pregnant and non-pregnant ewes. Journal of Dairy Science, 87(2):350-358.

22- Schlumbohm, C., H. P. Sporleder., H. Gurtler., and J. Harmeyer. 1997. The influence of insulin on metabolism of glucose, free fatty acids and glycerolin normo- and hypocalcaemic ewes during different reproductive states. Deutsch Tierarztl Wochenschr, 104(3):359–365.

23- Silanikove, N. 2000. Effects of heat stress on the welfare of extensively managed domestic ruminants. A review. Livestock Production Science, 67(1): 1-18.

24- Swanson, K. S., K. N. Kuzmuk., L. B. Schook., and G. C. Fahey. 2004. Diet affects nutrient digestibility, haematology, and serum chemistry of senior and weanling dogs. Journal of Animal Science, 82(2):1713-1724.

25- Tainturier, D., A. G. Braun Rico., and J. P. Thouvenot. 1984. Variation in blood composition in dairy cows during pregnancy and after calving. Research in Veterinary Science,37(2): 129-131.

26- Wang, X., and C. X. Hai. 2011. ROS acts as a double-edged sword in the pathogenesis of type 2 diabetes mellitus: is Nrf2 a potential target for the treatment? Mini-Reviews in Medicinal Chemistry, 11(12):1082–1092.

27- Yarim, G. F., and G. Ciftci. 2009. Serum protein pattern in ewe with pregnancy toxemia. Veterinary Research Communications, 33(5):431-438.

28- Yokus, B., D. U. Cakir., Z. Kanay., T. Gulten., and E. Uysal. 2006. Effects of seasonal and physiological variations on the serum chemistry, vitamins and thyroid hormone concentrations in sheep. Journal of Veterinary Medicine, 53(6):271-276.