Molecular Evaluation of Resistant to Aryloxyphenoxypropionate Herbicides in

پژوهشهای زراعی ایران

Article 2, Volume 10, Issue 2 - Serial Number 26, July 2012, Pages 270-276   

Document Type: Research Article

DOI: 10.22067/gsc.v10i2.16038

Authors

M Rastgoo^* ; M.H Rashed Mohasel; H. R Kavoosi; A Mirshamsi Kakhki; E Zand

Abstract

Molecular experiment was conducted to confirm resistant to aryloxyphenoxypropionate herbicides in winter wild oat (Avena ludoviciana Duriu.) populations of wheat fields in Khuzestan province, at Faculty of Agriculture of Ferdowsi University, during year of 2006. First, 44 wild oat populations from Ahvaz, Andimeshk, Shush, Shushtar, Ramhormoz, Susangerd, and Dezful cities with a susceptible population were examined in screening experiments. According to these experiments, 37 populations of winter wild oat biotypes selected as suspected resistance populations, and used for molecular experiment to determine resistance mechanism. dCAPS method was carried out in three steps consisting of PCR, enzyme digestion, and gel electrophoresis. Results showed that mutation at position 1781 of ACCase enzyme resulted in resistance in 10 winter wild oat populations from Andimeshk (5), Shush (1), Shushtar (2), and Susangerd (2) to clodinafop propargyl, diclofop methyl, and fenoxaprop p ethyl herbicides. While, in other suspected resistance populations other mechanism such as metabolism is probable. Also, it was concluded that this method is a good technique to detect herbicide resistance and mechanism of herbicide resistance in winter wild oat.

Keywords

ACCase inhibitors; Metabolism based resistance; Target site based resistance