The Effect of Salinity Stress on the Growth, quantity and quality of Essential oil of Lavender (Lavandula angustifulia Miller)

علوم باغبانی

Article 6, Volume 30, Issue 2, April 2016, Pages 209-216   

Document Type: Research Article

DOI: 10.22067/jhorts4.v30i2.38451

Authors

Sara Khorasaninejad^{* 1}; Hassan Soltanloo¹; Javad Hadian²; Sadegh Atashi³

¹Gorgan University of Agricultural Sciences and Natural Resources

²Medicinal Plant Research Istitude of Shahid Beheshti University

³Gorgan

Abstract

Introduction: Plants are usually exposed to different environmental stresses which limit their growth and productivity as well as cause considerable loss of worldwide agricultural production. One of the most important factors affecting plant and production of secondary metabolites is the salt stress. Salinity of soil or water is one of major stress, obstacles to increase production in plant growing areas throughout the world and especially in arid and semi-arid regions it can severely limit plant production. Iran is among the world's arid and semi-arid land, and faces water resources shortage and saline lands. According to the concept of sustainable development and role of Agriculture, using saline water and soil resources seems mandatory. Recently, medicinal and aromatic plants have received much attention in several fields such as agroalimentary, perfumes, pharmaceutical industries and natural cosmetic products. Although, secondary metabolites in the medicinal and aromatic plants were fundamentally produced by genetic processing, but, their biosynthesis are strongly influenced by environmental factors. It means that biotic and abiotic environmental factors affect growth parameter, essential oil yield and constituents. Abiotic environmental stresses, especially salinity and drought have the most effect on medicinal plant. Medicinal plants cultivation is one of ways to exploit these resources. Essential oils help to easier adapt to the environmental stress conditions. Also, essential oils are not constantly in the quantitative and qualitative terms. They are changing continuously, due to the requirements of the environment, and to individual survival. The different results were dedicated from the effect of salinity stress on the quantitative and qualitative parameters. Lavender (Lavandula angustifulia Miller) is a perennial woody medicinal plant that cultivated for its an essential oil in leafs and flowers. Major parts of Lavender produces essential oil are flowers and leaves.
Materials and Methods: This experiment was carried out using a randomized complete block design with three replications to study the effect of salinity stress on growth parameters, essential oil constituents and yield of Lavender (Lavandula angustifulia) at the Horticultural Sciences Department, Plant Product faculty, Gorgan Agricultural Sciences and Natural Resources University. Lavender plants were obtained from seed plantation. The seeds in this investigation were obtained from the Institution of Forests and Range researches in Tehran. After three weeks stratification (4ºC) and germination, five plants were transplanted into similarized pots that were filled with perlite and cocopeat (2:1). Irrigation treatments with hydroponic solution were completed during germination until stage of 6-8 leaf. Then, five levels of salt stress, including 0, 25, 50, 75 and 100 mM NaCl levels were investigated during four months, applied in hydroponic. Length, shoot wet weight, root wet weight and root dry weight were measured at full flowering stage (after five months). The same time in order to evaluate percentage and composition essential oil, each plant were harvested and dried under room condition. After two weeks, Clevenger method was used to extract the essential oil from the plant foliage. The obtained essential oil were measured for calculating of essential oils percentage and then, analyzed by using GC/MS (Gas choromatography-mass spectrometry) for identification and quantification of the components. Statistical analysis of data was used with SAS software and charts preparing was done with Excel software. Mean comparison with LSD’s test in 5 percent probability was used.
Results and Discussion: Results indicated that salinity stress motivated a significant influence in all of the growth parameters and essential oil yield and percent in P < 0.05. Increasing salt of the soil led to reduce in stem length, shoot wet weight, root wet weight and root dry weight and increase length of root and leaf essential oil percent at first, and then led to reduce these two parameters.
The highest values of the essential oil percent were obtained from second level of salinity. Also, it was reported that salt stress had a significant effect on yield and percent of essential oil that was similar to our finding. This was reported that percent of essential oil in each plant and composition of essential oil changed. According to the results of this research, salinity treatment significantly increased essential oil percentage in Lavandula angustifulia.
Conclusion: The most important of leaf essential oil component is Borneol that was increased. So, the medium level of salinity stress caused to increase in percent and quality of lavender essential oil.

Keywords

Borneol; growth parameters; GC-MS; Hydroponic; Medicinal plant

References

1- Abbasghlizadeh M. 2011. Effect of drought stress on quantity and quality characters of medicinal plant Nepeta racemosa. MSc thesis from Islamic Azad University-Rudehen unit. 86 pages.

2- Afzali S.F.A.D., Shariatmadari H., Hajiabbasi, M.A. and Moatar F. 2007. Salinity and drought stresses effects on flower yield and flavonol-o-glycosides in Chamomile (Matricaria chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants, 3 (37): 382-390 (in Persian).

3- Dow A.I., Cline, T.A. and Horning E.V. 1981. Salt tolerance studies on irrigated mint. Bull. Agr. Center, Washington State University, Pullman, No: 906.

4- El-Keltawi N.E. and Croteau R. 1987. Salinity depression of growth and essential oil formation in spearmint and marjoram and its reverse foliar applied cytokinin. Phytochemistry, 26:1333-1334.

5- Hajisamadiasl B., Hassanpouraghdam, M.B. and Khalighi A. 2011. Effects of gibberlic acid (GA3) foliar application on growth characteristics andessential oil of Lavender (Lavandula officinalis Chaix.), Journal of Agriculture Sciences. 21(2): 223 – 232.

6- Jaimand K. and Rezaei M.B. 2001. Essential oil and essential oil device. Researches of Medicinal and Aromatic Plants, 9: 1-161.

7- Khorasaninejad S., Mousavi A., Soltanloo H., Hemmati, K. and Khalighi A. 2010. The Effect of salinity stress on growth parameters, essential oil yield and constituent of Peppermint (Mentha piperita L.). World Applied Sciences Journal, 11 (11): 1403-1407.

8- Mashreghi M., Azizi M., Oroojalian, F. and Shah Tahmasebi N. 2014. Study on the chemical constituents and antibacterial activity of Kelussia odoratissima and Teucrium polium essential oils against some food borne pathogens. Journal of Horticultural Science, 28 (4): 487-495 (in Persian).

9- Mozaffarian V. 1996. A Dictionary of Iranian Plant Names. Farhang moaser, Tehran. 740p.

10- Nazari H., Jahanjo N., Safarieh M., Taherian M., Khaleghian, A. and Vafaei A. 2011. The effect of Avena sativa alcoholic and aqueous ewtract on the wound healing and skin inflammation. Urmia Medical Journal, 22 (5): 467-473.

11- Niakan M., Khavarynejad, R.A. and Rezaee M.B. 2004. Effects of different rates of NPK fertilizer on the leaf fresh weight, dry weight, leaf area and oil content of Mentha piperita L. Iranian Journal of Medicinal and Aromatic Plants Research, 20 (2): 131-148.

12- Noruzi M. 2001. The Hydroponic. Mohaddes Press.

13- Olfa Baatour R., Kaddour W., Aidi Wannes, M. and Lachaal Marzouk B. 2009. Salt effects on the growth, mineral nutrition, essential oil yield and composition of marjoram (Origanum majorana). Acta Physiol Plant, 10: 0374-4.

14- Omidbeigi R. 2005. Produce and process of medicinal plants. Volume 3. Astan Ghods Razavi Press. 400p.

15- Ozturk A., Unlukara A., Ipek, A. and Gurbuz B. 2004. Effects of salt stress and water deficit on plant growth and essential oil content of Lemon Balm (Melissa officialis L.). Pakistan Journal of Botany, 36(4): 787-792.

16- Prasad A., Anwar M., Patra, D.D. and Singh D.V. 1996. Tolerance of mint plants to soil salinity. Journal of the Indian Society of Soil Science, 44(1):184-186.

17- Safarnejad A., Sadr, S.V.A. and Hamidi H. 2007. Effect of salinity stress on morphological characters of Nigella sativa. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 15(1): 75- 84 (In Persian).

18- Safarnejad A. and Hamidi H. 2008. Morphological characterization of Foeniculum vulgare Mill. in response to salt stress, 17(1): 38-49.

19- Salami M.R., Safarnejad, A. and Hamidi H. 2006. Effect of salinity stress on morphological characters of Cuminum cyminum and Valeriana officinalis. Pajouhesh and Sazandegi, 72: 77 -83 (In Persian).

20- Sarani H., Heidari M., Glavi, M. and Siahsar B.A. 2013. Effects of salinity and iron on growth, photosynthetic pigments and electrophoresis bands in two genus chamomile (Matricaria chamomilla L. and Anthemis nobilis L.). Iranian Journal of Medicinal and Aromatic Plants, 29(4): 732-746 (In Persian).

21- Statistics letter of agriculture. 2011. Volume 2. Ministry of Agricultural Jahad.

22-The plan of guideline of Medicinal plant Research. 2008. Research Institute of Forestes and Rangelands.

23- The Site of Medical Services of Iran, WWW.IranEMs.com

24- Szabolcs I. 1989. Salt-affected soils. CRC press. Boca Raton. Florida. p 274.

25- Udagawa Y., Ito T., Tognoni F., Namiki T., Nukaya, H. and Maruo T. 1995. Some response of dill (Anettum graveolens) and thyme (Thymus vulgaris L.) grown in hydroponics to the concentration of nutrient solution. Acta Horticulturae, 396:203-210.

26- Upson T. and Andrews S. 2004. The genus Lavandula, 1st edn. Timber press, Portland, Oregon.

27- Zafari F., Amiri, M.E. and Vatanpour Azghandi A. 2015. Physiological response of pear (Pyrus Communis cv. Dargazi) to salinity stress under In vitro conditions. Journal of Horticultural Science, 28(4): 594-599 (in Persian).